Selective Co‐Assembly of Water‐Soluble Conjugated Polyelectrolyte with Discernable Fluorescence Modulation for Antibiotic Detection
Abstract Residual antibiotics threaten ecosystems and public health by fostering antibiotic resistance and water contamination. To address this, PQx‐Ph, a water‐soluble conjugated polyelectrolyte, is developed as a selective fluorescent probe for antibiotic detection. The sulfonate‐functionalized ar...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley-VCH
2025-07-01
|
| Series: | Advanced Sensor Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/adsr.70013 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1839632956149202944 |
|---|---|
| author | Hyena Ji Yesica Fernanda Florez‐Villabona Youngsun Kim Yerim Kim Yongju Kim Seungyun Baik Young Hun Seo Sehoon Kim |
| author_facet | Hyena Ji Yesica Fernanda Florez‐Villabona Youngsun Kim Yerim Kim Yongju Kim Seungyun Baik Young Hun Seo Sehoon Kim |
| author_sort | Hyena Ji |
| collection | DOAJ |
| description | Abstract Residual antibiotics threaten ecosystems and public health by fostering antibiotic resistance and water contamination. To address this, PQx‐Ph, a water‐soluble conjugated polyelectrolyte, is developed as a selective fluorescent probe for antibiotic detection. The sulfonate‐functionalized architecture of PQx‐Ph facilitates the molecular co‐assembly with antibiotics, resulting in fluorescence responses through antibiotic‐selective distinct mechanisms, i.e., aggregation‐induced emission (AIE) and twisted intramolecular charge transfer (TICT). These antibiotic‐selective mechanisms enable 1) fluorescence quenching upon co‐assembly with kanamycin (KAN) due to electrostatic binding and 2) fluorescence enhancement upon co‐assembly with erythromycin (ERY) through hydrophobic interactions and TICT stabilization. PQx‐Ph exhibits remarkable selectivity toward KAN, demonstrating strong binding with minimal interference from various competing substances. PQx‐Ph exhibits matrix‐dependent sensing performance, achieving nanomolar‐level KAN detection (LOD = 0.021 µm) in mild environments containing low levels of natural organic matter (NOM), and maintaining reliable micromolar‐level sensitivity (LOD = 0.37–1.44 µm) in environmentally complex matrices such as synthetic urine, tap water, and NOM‐rich water samples. Given its excellent water solubility, environmental stability, and structural adaptability, PQx‐Ph emerges as a promising candidate for real‐time monitoring of antibiotic contamination. Future integration into portable sensing platforms will broaden its applications in environmental and public health monitoring. |
| format | Article |
| id | doaj-art-7169656b0d5147d5afc6a15a5edab399 |
| institution | Matheson Library |
| issn | 2751-1219 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Advanced Sensor Research |
| spelling | doaj-art-7169656b0d5147d5afc6a15a5edab3992025-07-11T06:16:52ZengWiley-VCHAdvanced Sensor Research2751-12192025-07-0147n/an/a10.1002/adsr.70013Selective Co‐Assembly of Water‐Soluble Conjugated Polyelectrolyte with Discernable Fluorescence Modulation for Antibiotic DetectionHyena Ji0Yesica Fernanda Florez‐Villabona1Youngsun Kim2Yerim Kim3Yongju Kim4Seungyun Baik5Young Hun Seo6Sehoon Kim7Energy & Environment Cluster, Planning and Coordination Division Korea Institute of Science and Technology Europe Campus E7.1 66123 Saarbrücken GermanyEnergy & Environment Cluster, Planning and Coordination Division Korea Institute of Science and Technology Europe Campus E7.1 66123 Saarbrücken GermanyChemical and Biological Integrative Research Center Korea Institute of Science and Technology (KIST) Seoul 02792 Republic of KoreaChemical and Biological Integrative Research Center Korea Institute of Science and Technology (KIST) Seoul 02792 Republic of KoreaKU‐KIST Graduate School of Converging Science and Technology Korea University Seoul 02841 Republic of KoreaEnergy & Environment Cluster, Planning and Coordination Division Korea Institute of Science and Technology Europe Campus E7.1 66123 Saarbrücken GermanyEnergy & Environment Cluster, Planning and Coordination Division Korea Institute of Science and Technology Europe Campus E7.1 66123 Saarbrücken GermanyChemical and Biological Integrative Research Center Korea Institute of Science and Technology (KIST) Seoul 02792 Republic of KoreaAbstract Residual antibiotics threaten ecosystems and public health by fostering antibiotic resistance and water contamination. To address this, PQx‐Ph, a water‐soluble conjugated polyelectrolyte, is developed as a selective fluorescent probe for antibiotic detection. The sulfonate‐functionalized architecture of PQx‐Ph facilitates the molecular co‐assembly with antibiotics, resulting in fluorescence responses through antibiotic‐selective distinct mechanisms, i.e., aggregation‐induced emission (AIE) and twisted intramolecular charge transfer (TICT). These antibiotic‐selective mechanisms enable 1) fluorescence quenching upon co‐assembly with kanamycin (KAN) due to electrostatic binding and 2) fluorescence enhancement upon co‐assembly with erythromycin (ERY) through hydrophobic interactions and TICT stabilization. PQx‐Ph exhibits remarkable selectivity toward KAN, demonstrating strong binding with minimal interference from various competing substances. PQx‐Ph exhibits matrix‐dependent sensing performance, achieving nanomolar‐level KAN detection (LOD = 0.021 µm) in mild environments containing low levels of natural organic matter (NOM), and maintaining reliable micromolar‐level sensitivity (LOD = 0.37–1.44 µm) in environmentally complex matrices such as synthetic urine, tap water, and NOM‐rich water samples. Given its excellent water solubility, environmental stability, and structural adaptability, PQx‐Ph emerges as a promising candidate for real‐time monitoring of antibiotic contamination. Future integration into portable sensing platforms will broaden its applications in environmental and public health monitoring.https://doi.org/10.1002/adsr.70013aggregation‐induced emissionantibioticsco‐assemblyconjugated polyelectrolytefluorescence detection |
| spellingShingle | Hyena Ji Yesica Fernanda Florez‐Villabona Youngsun Kim Yerim Kim Yongju Kim Seungyun Baik Young Hun Seo Sehoon Kim Selective Co‐Assembly of Water‐Soluble Conjugated Polyelectrolyte with Discernable Fluorescence Modulation for Antibiotic Detection Advanced Sensor Research aggregation‐induced emission antibiotics co‐assembly conjugated polyelectrolyte fluorescence detection |
| title | Selective Co‐Assembly of Water‐Soluble Conjugated Polyelectrolyte with Discernable Fluorescence Modulation for Antibiotic Detection |
| title_full | Selective Co‐Assembly of Water‐Soluble Conjugated Polyelectrolyte with Discernable Fluorescence Modulation for Antibiotic Detection |
| title_fullStr | Selective Co‐Assembly of Water‐Soluble Conjugated Polyelectrolyte with Discernable Fluorescence Modulation for Antibiotic Detection |
| title_full_unstemmed | Selective Co‐Assembly of Water‐Soluble Conjugated Polyelectrolyte with Discernable Fluorescence Modulation for Antibiotic Detection |
| title_short | Selective Co‐Assembly of Water‐Soluble Conjugated Polyelectrolyte with Discernable Fluorescence Modulation for Antibiotic Detection |
| title_sort | selective co assembly of water soluble conjugated polyelectrolyte with discernable fluorescence modulation for antibiotic detection |
| topic | aggregation‐induced emission antibiotics co‐assembly conjugated polyelectrolyte fluorescence detection |
| url | https://doi.org/10.1002/adsr.70013 |
| work_keys_str_mv | AT hyenaji selectivecoassemblyofwatersolubleconjugatedpolyelectrolytewithdiscernablefluorescencemodulationforantibioticdetection AT yesicafernandaflorezvillabona selectivecoassemblyofwatersolubleconjugatedpolyelectrolytewithdiscernablefluorescencemodulationforantibioticdetection AT youngsunkim selectivecoassemblyofwatersolubleconjugatedpolyelectrolytewithdiscernablefluorescencemodulationforantibioticdetection AT yerimkim selectivecoassemblyofwatersolubleconjugatedpolyelectrolytewithdiscernablefluorescencemodulationforantibioticdetection AT yongjukim selectivecoassemblyofwatersolubleconjugatedpolyelectrolytewithdiscernablefluorescencemodulationforantibioticdetection AT seungyunbaik selectivecoassemblyofwatersolubleconjugatedpolyelectrolytewithdiscernablefluorescencemodulationforantibioticdetection AT younghunseo selectivecoassemblyofwatersolubleconjugatedpolyelectrolytewithdiscernablefluorescencemodulationforantibioticdetection AT sehoonkim selectivecoassemblyofwatersolubleconjugatedpolyelectrolytewithdiscernablefluorescencemodulationforantibioticdetection |