Production of single-cell protein by Candida Utilis and Komagataella Pastoris from enzymatically hydrolyzed defatted soybean meal
Defatted soybean meal (D-SBM), a byproduct of soybean oil production, offers high protein content but is limited in its effectiveness as feed to non-ruminant animals due to its indigestible carbohydrate fractions. This study investigates the performance of enzymatic hydrolysis of D-SBM to convert it...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-08-01
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| Series: | Journal of Agriculture and Food Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666154325004399 |
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| Summary: | Defatted soybean meal (D-SBM), a byproduct of soybean oil production, offers high protein content but is limited in its effectiveness as feed to non-ruminant animals due to its indigestible carbohydrate fractions. This study investigates the performance of enzymatic hydrolysis of D-SBM to convert its carbohydrates into monomeric sugars, followed by their utilization as a carbon source to produce single-cell protein (SCP) using Candida utilis and Komagataella pastoris. Optimized enzymatic hydrolysis with 22 mg protein·g−1 D-SBM of total enzyme loading achieved a total monomeric sugar yield of 33.0 ± 1.6 %. Cell propagation experiments demonstrated that K. pastoris and C. utilis utilized enzymatic hydrolysis-derived hexoses, namely glucose, as carbon sources. Both yeasts reached maximum growth rates between 2 and 8 h of cultivation, reaching maximum specific growth rates of 0.268 h−1 and 0.177 h−1, respectively. Single cell production was efficient, with yields of approximately 1.3 g protein·g−1 of monomeric sugar consumed, resulting in SCP contents of approximately 41 % from both yeasts. Amino acid analysis revealed a significant improvement in lysine and methionine levels of 67 % and 52 %, respectively, in crude protein from K. pastoris relative to D-SBM.This study highlights the potential of D-SBM as a sustainable feedstock for SCP production, providing an innovative approach to repurpose agricultural feedstocks into nutritionally higher quality protein. This transformation can potentially contribute to the development of cost-effective and environmentally friendly alternatives to conventional animal protein sources. |
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| ISSN: | 2666-1543 |