The Quantitative Detection of Urogenital Mycoplasmas in Men with Urolithiasis

Urease-positive urogenital mycoplasmas are considered to be responsible for the formation of urinary stones. They are usually a part of the normal flora in the human urogenital tract, causing asymptomatic infections. However, many symptomatic infections with these bacteria have been reported. <i&...

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Autori principali: Dominika Smolec, Małgorzata Aptekorz, Łukasz Filipczyk, Zygmunt Gofron, Jacek Zostawa, Robert Smolec, Tomasz J. Wąsik, Alicja Ekiel
Natura: Articolo
Lingua:inglese
Pubblicazione: MDPI AG 2025-07-01
Serie:Pathogens
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Accesso online:https://www.mdpi.com/2076-0817/14/7/670
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Riassunto:Urease-positive urogenital mycoplasmas are considered to be responsible for the formation of urinary stones. They are usually a part of the normal flora in the human urogenital tract, causing asymptomatic infections. However, many symptomatic infections with these bacteria have been reported. <i>M. genitalium</i> is recognized as a cause of male urethritis and other common genitourinary diseases. The role of other urogenital mycoplasmas is still unclear. The aim of this study was to estimate the quantitative prevalence of <i>Ureaplasma</i> spp., <i>M. genitalium</i> and <i>M. hominis</i> in men with urolithiasis using quantitative real-time PCR (qPCR). The study group comprised 100 men with urolithiasis. A total of 60 men were included in the control group. Urogenital mycoplasma DNA in urine samples was detected significantly more often among men with urolithiasis than in healthy subjects—43.0% vs. 26.6%, <i>p</i> = 0.0382, respectively. The majority of positive results (38/43) concerned <i>U. parvum</i> species, the frequency of which was higher in the study group (38.0% (38/100)) than in the control group (23.3% (14/60)), <i>p</i> = 0.0552. The median concentration of <i>U. urealyticum</i> DNA was higher in the study group compared with the control, <i>p</i> = 0.5714. However, further studies are needed to confirm the usefulness of quantitative studies in determining the role of urogenital mycoplasmas in pathology.
ISSN:2076-0817