<i>Undaria pinnatifida</i> Fucoidan Enhances Gut Microbiome, Butyrate Production, and Exerts Anti-Inflammatory Effects in an In Vitro Short-Term SHIME<sup>®</sup> Coupled to a Caco-2/THP-1 Co-Culture Model
Fucoidans have demonstrated a wide range of bioactivities including immune modulation and benefits in gut health. To gain a deeper understanding on the effects of fucoidan from <i>Undaria pinnatifida</i> (UPF) on the colonic microbiome, the short-term Simulator of the Human Intestinal Mi...
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MDPI AG
2025-06-01
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| Online Access: | https://www.mdpi.com/1660-3397/23/6/242 |
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| author | Barbara C. Wimmer Corinna Dwan Jelle De Medts Cindy Duysburgh Chloë Rotsaert Massimo Marzorati |
| author_facet | Barbara C. Wimmer Corinna Dwan Jelle De Medts Cindy Duysburgh Chloë Rotsaert Massimo Marzorati |
| author_sort | Barbara C. Wimmer |
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| description | Fucoidans have demonstrated a wide range of bioactivities including immune modulation and benefits in gut health. To gain a deeper understanding on the effects of fucoidan from <i>Undaria pinnatifida</i> (UPF) on the colonic microbiome, the short-term Simulator of the Human Intestinal Microbial Ecosystem<sup>®</sup>, a validated in vitro gut model, was applied. Following a three-week intervention period on adult faecal samples from three healthy donors, microbial community activity of the colonic microbiota was assessed by quantifying short-chain fatty acids while composition was analysed utilising 16S-targeted Illumina sequencing. Metagenomic data were used to describe changes in community structure. To assess the secretion of cytokines, co-culture experiments using Caco-2 and THP1-Blue™ cells were performed. UPF supplementation over a three-week period had a profound butyrogenic effect while also enriching colonic microbial diversity, consistently stimulating saccharolytic genera, and reducing genera linked with potentially negative health effects in both regions of the colon. Mild immune modulatory effects of UPF were also observed. Colonic fermentation of UPF showed anti-inflammatory properties by inducing the secretion of the anti-inflammatory cytokines IL-6 and IL-10 in two out of three donors in the proximal and distal colon. In conclusion, UPF supplementation may provide significant gut health benefits. |
| format | Article |
| id | doaj-art-6e5ce8c2525747588dcad8b4c6f2751a |
| institution | Matheson Library |
| issn | 1660-3397 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
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| series | Marine Drugs |
| spelling | doaj-art-6e5ce8c2525747588dcad8b4c6f2751a2025-06-25T14:08:07ZengMDPI AGMarine Drugs1660-33972025-06-0123624210.3390/md23060242<i>Undaria pinnatifida</i> Fucoidan Enhances Gut Microbiome, Butyrate Production, and Exerts Anti-Inflammatory Effects in an In Vitro Short-Term SHIME<sup>®</sup> Coupled to a Caco-2/THP-1 Co-Culture ModelBarbara C. Wimmer0Corinna Dwan1Jelle De Medts2Cindy Duysburgh3Chloë Rotsaert4Massimo Marzorati5Marinova Pty Ltd., 249 Kennedy Drive, Cambridge, TAS 7170, AustraliaMarinova Pty Ltd., 249 Kennedy Drive, Cambridge, TAS 7170, AustraliaProDigest BV, Technologiepark 82, 9052 Ghent, BelgiumProDigest BV, Technologiepark 82, 9052 Ghent, BelgiumProDigest BV, Technologiepark 82, 9052 Ghent, BelgiumProDigest BV, Technologiepark 82, 9052 Ghent, BelgiumFucoidans have demonstrated a wide range of bioactivities including immune modulation and benefits in gut health. To gain a deeper understanding on the effects of fucoidan from <i>Undaria pinnatifida</i> (UPF) on the colonic microbiome, the short-term Simulator of the Human Intestinal Microbial Ecosystem<sup>®</sup>, a validated in vitro gut model, was applied. Following a three-week intervention period on adult faecal samples from three healthy donors, microbial community activity of the colonic microbiota was assessed by quantifying short-chain fatty acids while composition was analysed utilising 16S-targeted Illumina sequencing. Metagenomic data were used to describe changes in community structure. To assess the secretion of cytokines, co-culture experiments using Caco-2 and THP1-Blue™ cells were performed. UPF supplementation over a three-week period had a profound butyrogenic effect while also enriching colonic microbial diversity, consistently stimulating saccharolytic genera, and reducing genera linked with potentially negative health effects in both regions of the colon. Mild immune modulatory effects of UPF were also observed. Colonic fermentation of UPF showed anti-inflammatory properties by inducing the secretion of the anti-inflammatory cytokines IL-6 and IL-10 in two out of three donors in the proximal and distal colon. In conclusion, UPF supplementation may provide significant gut health benefits.https://www.mdpi.com/1660-3397/23/6/242fucoidans<i>Undaria pinnatifida</i>butyrategastrointestinal microbiomeSHIMEgut health |
| spellingShingle | Barbara C. Wimmer Corinna Dwan Jelle De Medts Cindy Duysburgh Chloë Rotsaert Massimo Marzorati <i>Undaria pinnatifida</i> Fucoidan Enhances Gut Microbiome, Butyrate Production, and Exerts Anti-Inflammatory Effects in an In Vitro Short-Term SHIME<sup>®</sup> Coupled to a Caco-2/THP-1 Co-Culture Model Marine Drugs fucoidans <i>Undaria pinnatifida</i> butyrate gastrointestinal microbiome SHIME gut health |
| title | <i>Undaria pinnatifida</i> Fucoidan Enhances Gut Microbiome, Butyrate Production, and Exerts Anti-Inflammatory Effects in an In Vitro Short-Term SHIME<sup>®</sup> Coupled to a Caco-2/THP-1 Co-Culture Model |
| title_full | <i>Undaria pinnatifida</i> Fucoidan Enhances Gut Microbiome, Butyrate Production, and Exerts Anti-Inflammatory Effects in an In Vitro Short-Term SHIME<sup>®</sup> Coupled to a Caco-2/THP-1 Co-Culture Model |
| title_fullStr | <i>Undaria pinnatifida</i> Fucoidan Enhances Gut Microbiome, Butyrate Production, and Exerts Anti-Inflammatory Effects in an In Vitro Short-Term SHIME<sup>®</sup> Coupled to a Caco-2/THP-1 Co-Culture Model |
| title_full_unstemmed | <i>Undaria pinnatifida</i> Fucoidan Enhances Gut Microbiome, Butyrate Production, and Exerts Anti-Inflammatory Effects in an In Vitro Short-Term SHIME<sup>®</sup> Coupled to a Caco-2/THP-1 Co-Culture Model |
| title_short | <i>Undaria pinnatifida</i> Fucoidan Enhances Gut Microbiome, Butyrate Production, and Exerts Anti-Inflammatory Effects in an In Vitro Short-Term SHIME<sup>®</sup> Coupled to a Caco-2/THP-1 Co-Culture Model |
| title_sort | i undaria pinnatifida i fucoidan enhances gut microbiome butyrate production and exerts anti inflammatory effects in an in vitro short term shime sup r sup coupled to a caco 2 thp 1 co culture model |
| topic | fucoidans <i>Undaria pinnatifida</i> butyrate gastrointestinal microbiome SHIME gut health |
| url | https://www.mdpi.com/1660-3397/23/6/242 |
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