Phenotype Characterization of Beta-Lactamase Producing Enterobacteriaceae in the Intensive Care Unit (ICU) of Cipto Mangunkusumo Hospital in 2011
Aim: the goal of this study is to understand the phenotype characteristic of beta-lactamase enzymes producing Enterobacteriaceae, such as ESBL, AmpC, and carbapenemase. Methods: three different methods are performed to confirm those phenotypic characteristics, namely double disk diffusion method to...
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| Format: | Article |
| Language: | English |
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Interna Publishing
2016-05-01
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| Series: | Acta Medica Indonesiana |
| Online Access: | https://actamedindones.org/index.php/ijim/article/view/115 |
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| _version_ | 1839618198257795072 |
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| author | Yulia R Saharman Delly C Lestari |
| author_facet | Yulia R Saharman Delly C Lestari |
| author_sort | Yulia R Saharman |
| collection | DOAJ |
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Aim: the goal of this study is to understand the phenotype characteristic of beta-lactamase enzymes producing Enterobacteriaceae, such as ESBL, AmpC, and carbapenemase. Methods: three different methods are performed to confirm those phenotypic characteristics, namely double disk diffusion method to confirm ESBL, AmpC disk test (cefoxitin-based) to confirm AmpC, and modified Hodge test to confirm carbapenemase. Results: using double disk diffusion method, we found 58.42% isolates are ESBL-producing, whereas the outcomes of AmpC disk test shows 1.98% are AmpC-producing. By conducting modified Hodge test (MHT), 27.59% isolates are confirmed as carbapenemase-producing bacteria. Conclusion: this study confirmed the prevalence of beta-lactamase producing Klebsiella pneumoniae is very high. Nevertheless, AmpC and carbapenemase should not be ignored despite their low prevalence.
Key words: Enterobacteriaceae, antibiotic resistance, ESBL, AmpC, carbapenemase.
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| format | Article |
| id | doaj-art-6d93b307e6b5445d9d6ebe1a0269d23c |
| institution | Matheson Library |
| issn | 0125-9326 2338-2732 |
| language | English |
| publishDate | 2016-05-01 |
| publisher | Interna Publishing |
| record_format | Article |
| series | Acta Medica Indonesiana |
| spelling | doaj-art-6d93b307e6b5445d9d6ebe1a0269d23c2025-07-24T07:02:57ZengInterna PublishingActa Medica Indonesiana0125-93262338-27322016-05-01451Phenotype Characterization of Beta-Lactamase Producing Enterobacteriaceae in the Intensive Care Unit (ICU) of Cipto Mangunkusumo Hospital in 2011Yulia R Saharman0Delly C Lestari1Department of Microbiology, Faculty of Medicine, University of Indonesia – Cipto Mangunkusumo HospitalDepartment of Microbiology, Faculty of Medicine, University of Indonesia – Cipto Mangunkusumo Hospital Aim: the goal of this study is to understand the phenotype characteristic of beta-lactamase enzymes producing Enterobacteriaceae, such as ESBL, AmpC, and carbapenemase. Methods: three different methods are performed to confirm those phenotypic characteristics, namely double disk diffusion method to confirm ESBL, AmpC disk test (cefoxitin-based) to confirm AmpC, and modified Hodge test to confirm carbapenemase. Results: using double disk diffusion method, we found 58.42% isolates are ESBL-producing, whereas the outcomes of AmpC disk test shows 1.98% are AmpC-producing. By conducting modified Hodge test (MHT), 27.59% isolates are confirmed as carbapenemase-producing bacteria. Conclusion: this study confirmed the prevalence of beta-lactamase producing Klebsiella pneumoniae is very high. Nevertheless, AmpC and carbapenemase should not be ignored despite their low prevalence. Key words: Enterobacteriaceae, antibiotic resistance, ESBL, AmpC, carbapenemase. https://actamedindones.org/index.php/ijim/article/view/115 |
| spellingShingle | Yulia R Saharman Delly C Lestari Phenotype Characterization of Beta-Lactamase Producing Enterobacteriaceae in the Intensive Care Unit (ICU) of Cipto Mangunkusumo Hospital in 2011 Acta Medica Indonesiana |
| title | Phenotype Characterization of Beta-Lactamase Producing Enterobacteriaceae in the Intensive Care Unit (ICU) of Cipto Mangunkusumo Hospital in 2011 |
| title_full | Phenotype Characterization of Beta-Lactamase Producing Enterobacteriaceae in the Intensive Care Unit (ICU) of Cipto Mangunkusumo Hospital in 2011 |
| title_fullStr | Phenotype Characterization of Beta-Lactamase Producing Enterobacteriaceae in the Intensive Care Unit (ICU) of Cipto Mangunkusumo Hospital in 2011 |
| title_full_unstemmed | Phenotype Characterization of Beta-Lactamase Producing Enterobacteriaceae in the Intensive Care Unit (ICU) of Cipto Mangunkusumo Hospital in 2011 |
| title_short | Phenotype Characterization of Beta-Lactamase Producing Enterobacteriaceae in the Intensive Care Unit (ICU) of Cipto Mangunkusumo Hospital in 2011 |
| title_sort | phenotype characterization of beta lactamase producing enterobacteriaceae in the intensive care unit icu of cipto mangunkusumo hospital in 2011 |
| url | https://actamedindones.org/index.php/ijim/article/view/115 |
| work_keys_str_mv | AT yuliarsaharman phenotypecharacterizationofbetalactamaseproducingenterobacteriaceaeintheintensivecareuniticuofciptomangunkusumohospitalin2011 AT dellyclestari phenotypecharacterizationofbetalactamaseproducingenterobacteriaceaeintheintensivecareuniticuofciptomangunkusumohospitalin2011 |