INDICATION OF THE BIOCHEMICAL TARGETS OF RARE EARTH ELEMENTS IN BIVALVE MOLLUSCS UNDER MULTIPLE EXPOSURE
Rare earth elements (REE) are considered the pollutants of priority, and biological effects are studied poorly. Aim. Our study was devoted to the elucidation of suspected biochemical effects of two representative REEs, gadolinium (Gd) and yttrium (Y), individually and in a mixture with Ca-channel...
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2025-04-01
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| Series: | Biotechnologia Acta |
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| Online Access: | https://biotechnology.kiev.ua/images/BTA/2025/2_2025/Khoma2_2025%20.pdf |
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| author | V. Khoma K. Yunko V. Martyniuk M.Zarian V. Havryshkiv |
| author_facet | V. Khoma K. Yunko V. Martyniuk M.Zarian V. Havryshkiv |
| author_sort | V. Khoma |
| collection | DOAJ |
| description | Rare earth elements (REE) are considered the pollutants of priority, and biological effects are studied poorly.
Aim. Our study was devoted to the elucidation of suspected biochemical effects of two representative REEs, gadolinium (Gd) and yttrium (Y), individually and in a mixture with Ca-channel blocker nifedipine (Nfd), using the freshwater bivalve Unio tumidus as a model organism.
Materials and Methods. The specimens of swollen river mussel Unio tumidus were treated with GdCl3, 30 nM, YCl3, 30 nM, or their mixture with Nfd (10 µM) for 14 days. The set of metal-binding, reductive state, biotransformation activities, and toxicity in the digestive gland of mussels was selected.
Results. All exposures caused the elevation of the ratio of reduced and oxidized forms of nicotinamide adenine dinucleotide (NADH/NAD+ ratio) and increase of the levels of reduced and oxidized glutathione (GSH and GSSG), the total level of cysteine-rich protein metallothionein (MTSH) in the Gd- and Y- groups and non-metalated MTSH in all exposures. The effects on the transformation of Phase I and Phase II enzymes were exposure-dependent. Dynamin-related GTP-ase activity increased in all exposures. The lysosomal membrane integrity and apoptotic activity decreased only in the Gd-group.
Conclusion. REEs in low concentrations cause adaptive metabolic responses in the mussels. The changes in the Zn distribution within the cells and Zn-dependent functions were indicated, confirming the impact of REEs on the functionality of the essential metal. In the combined exposure of REEs with a Ca-channel blocker, a cumulative effect was detected. |
| format | Article |
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| institution | Matheson Library |
| issn | 2410-7751 2410-776X |
| language | English |
| publishDate | 2025-04-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
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| series | Biotechnologia Acta |
| spelling | doaj-art-6cb2fbd0fe164059b10cb77bb9113f492025-06-24T15:12:39ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryBiotechnologia Acta2410-77512410-776X2025-04-01182454710.15407/biotech18.02.045INDICATION OF THE BIOCHEMICAL TARGETS OF RARE EARTH ELEMENTS IN BIVALVE MOLLUSCS UNDER MULTIPLE EXPOSUREV. Khoma0 K. Yunko 1 V. Martyniuk2M.Zarian 3V. Havryshkiv4Ternopil Scientific Research Forensic Center of the Ministry of Internal Affairs of UkraineTernopil Volodymyr Hnatiuk National Pedagogical University, Ukraine Ternopil Ivan Puluj National Technical University, UkraineNature Research Centre, Vilnius, Lietuvos RespublikaTernopil Volodymyr Hnatiuk National Pedagogical University, UkraineRare earth elements (REE) are considered the pollutants of priority, and biological effects are studied poorly. Aim. Our study was devoted to the elucidation of suspected biochemical effects of two representative REEs, gadolinium (Gd) and yttrium (Y), individually and in a mixture with Ca-channel blocker nifedipine (Nfd), using the freshwater bivalve Unio tumidus as a model organism. Materials and Methods. The specimens of swollen river mussel Unio tumidus were treated with GdCl3, 30 nM, YCl3, 30 nM, or their mixture with Nfd (10 µM) for 14 days. The set of metal-binding, reductive state, biotransformation activities, and toxicity in the digestive gland of mussels was selected. Results. All exposures caused the elevation of the ratio of reduced and oxidized forms of nicotinamide adenine dinucleotide (NADH/NAD+ ratio) and increase of the levels of reduced and oxidized glutathione (GSH and GSSG), the total level of cysteine-rich protein metallothionein (MTSH) in the Gd- and Y- groups and non-metalated MTSH in all exposures. The effects on the transformation of Phase I and Phase II enzymes were exposure-dependent. Dynamin-related GTP-ase activity increased in all exposures. The lysosomal membrane integrity and apoptotic activity decreased only in the Gd-group. Conclusion. REEs in low concentrations cause adaptive metabolic responses in the mussels. The changes in the Zn distribution within the cells and Zn-dependent functions were indicated, confirming the impact of REEs on the functionality of the essential metal. In the combined exposure of REEs with a Ca-channel blocker, a cumulative effect was detected.https://biotechnology.kiev.ua/images/BTA/2025/2_2025/Khoma2_2025%20.pdfgadoliniumyttriummultiple exposuremussels |
| spellingShingle | V. Khoma K. Yunko V. Martyniuk M.Zarian V. Havryshkiv INDICATION OF THE BIOCHEMICAL TARGETS OF RARE EARTH ELEMENTS IN BIVALVE MOLLUSCS UNDER MULTIPLE EXPOSURE Biotechnologia Acta gadolinium yttrium multiple exposure mussels |
| title | INDICATION OF THE BIOCHEMICAL TARGETS OF RARE EARTH ELEMENTS IN BIVALVE MOLLUSCS UNDER MULTIPLE EXPOSURE |
| title_full | INDICATION OF THE BIOCHEMICAL TARGETS OF RARE EARTH ELEMENTS IN BIVALVE MOLLUSCS UNDER MULTIPLE EXPOSURE |
| title_fullStr | INDICATION OF THE BIOCHEMICAL TARGETS OF RARE EARTH ELEMENTS IN BIVALVE MOLLUSCS UNDER MULTIPLE EXPOSURE |
| title_full_unstemmed | INDICATION OF THE BIOCHEMICAL TARGETS OF RARE EARTH ELEMENTS IN BIVALVE MOLLUSCS UNDER MULTIPLE EXPOSURE |
| title_short | INDICATION OF THE BIOCHEMICAL TARGETS OF RARE EARTH ELEMENTS IN BIVALVE MOLLUSCS UNDER MULTIPLE EXPOSURE |
| title_sort | indication of the biochemical targets of rare earth elements in bivalve molluscs under multiple exposure |
| topic | gadolinium yttrium multiple exposure mussels |
| url | https://biotechnology.kiev.ua/images/BTA/2025/2_2025/Khoma2_2025%20.pdf |
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