Enteric-coated microencapsulation improves the therapeutic effect of cinnamon oil on artificially infected chicken with Escherichia coli
Avian pathogenic Escherichia coli (APEC) is an important bacterial infectious disease can pose a threat to poultry and human health, with infected chicks typically exhibiting diarrhea and enteritis. Cinnamon essential oil (CEO) exhibits significant antibacterial, anti-inflammatory and intestinal reg...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-10-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579125008508 |
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| author | Huanling Xing Fang Zhou Jianhao Lin Junfeng Ban Junyi Huang Xinghai Li Hanjun He Yanqun Li Mei Bai Xiangxiu Liang Hong Wu |
| author_facet | Huanling Xing Fang Zhou Jianhao Lin Junfeng Ban Junyi Huang Xinghai Li Hanjun He Yanqun Li Mei Bai Xiangxiu Liang Hong Wu |
| author_sort | Huanling Xing |
| collection | DOAJ |
| description | Avian pathogenic Escherichia coli (APEC) is an important bacterial infectious disease can pose a threat to poultry and human health, with infected chicks typically exhibiting diarrhea and enteritis. Cinnamon essential oil (CEO) exhibits significant antibacterial, anti-inflammatory and intestinal regulatory properties but limited bioavailability. This study aims to develop an enteric CEO microcapsule and investigate its regulatory effects on anti-inflammatory, and intestinal microenvironment in E. coli-infected chickens, and to reveal the reasons for its superior therapeutic effect on CEO. Different concentrations of enteric CEO microcapsules and CEO were used to treat artificially infected chicken E. coli models, respectively. Cecal content was collected for 16S rRNA gene sequence analysis and metabonomics. Butyric acid content was detected by gas chromatography-flame ionization detection (GC-FID). Inflammatory factors and lipopolysaccharides (LPS) of serum were measured by corresponding ELISA kits. And inflammatory factors in ileum, jejunum and duodenum tissues were detected via quantitative real-time PCR (qRT‒PCR). Pathologic changes in hepatocytes and duodenum were detected by H&E. Dissolution of enteric CEO microcapsules was determined by the paddle method. We observed that the low-dose CEO microcapsule (APEC+MCL) with at least 75 % lower CEO content increased the bioavailability of CEO. This effect was associated with an improvement in the internal environment of the intestinal tract, as in increasing beneficial bacteria and reducing harmful bacteria. Meanwhile, APEC+MCL significantly increased butyric acid content and restored the gut metabolites, improved the level of intestinal inflammatory factors and reduced the inflammatory response, particularly in the duodenum and jejunum segments, with a significant increase in therapeutic effect. Furthermore, APEC+MCL also improved the levels of TNF-α, IL-1β, IL-6, and IL-10 in serum, as well as the liver's protective effect to reduce mortality and increase feed conversion rate (FCR) and average daily gain (ADG). These findings suggest enteric microcapsule system for CEO significantly enhances CEO's therapeutic potential, offering a promising strategy for developing more efficient and cost-effective natural antibacterial treatments for colibacillosis. |
| format | Article |
| id | doaj-art-6c8dff9c40a84c98a2b280e93fecc8fe |
| institution | Matheson Library |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-6c8dff9c40a84c98a2b280e93fecc8fe2025-08-03T04:42:10ZengElsevierPoultry Science0032-57912025-10-0110410105609Enteric-coated microencapsulation improves the therapeutic effect of cinnamon oil on artificially infected chicken with Escherichia coliHuanling Xing0Fang Zhou1Jianhao Lin2Junfeng Ban3Junyi Huang4Xinghai Li5Hanjun He6Yanqun Li7Mei Bai8Xiangxiu Liang9Hong Wu10Center for Medicinal Plants Research, College of Life Sciences, South China Agricultural University, Guangzhou 510642, PR China; Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agricultural University, Guangzhou 510642, PR China; Poultry Science, Mississippi State University, Mississippi State, MS 39762, USASericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, PR ChinaCenter for Medicinal Plants Research, College of Life Sciences, South China Agricultural University, Guangzhou 510642, PR China; Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agricultural University, Guangzhou 510642, PR ChinaGuangdong Provincial Key Laboratory of Advanced Drug Delivery Systems, Guangdong Pharmaceutical University, Guangzhou, PR ChinaCenter for Medicinal Plants Research, College of Life Sciences, South China Agricultural University, Guangzhou 510642, PR China; Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agricultural University, Guangzhou 510642, PR ChinaCenter for Medicinal Plants Research, College of Life Sciences, South China Agricultural University, Guangzhou 510642, PR China; Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agricultural University, Guangzhou 510642, PR ChinaCenter for Medicinal Plants Research, College of Life Sciences, South China Agricultural University, Guangzhou 510642, PR China; Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agricultural University, Guangzhou 510642, PR ChinaCenter for Medicinal Plants Research, College of Life Sciences, South China Agricultural University, Guangzhou 510642, PR China; Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agricultural University, Guangzhou 510642, PR ChinaCenter for Medicinal Plants Research, College of Life Sciences, South China Agricultural University, Guangzhou 510642, PR China; Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agricultural University, Guangzhou 510642, PR ChinaCenter for Medicinal Plants Research, College of Life Sciences, South China Agricultural University, Guangzhou 510642, PR China; Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agricultural University, Guangzhou 510642, PR ChinaCenter for Medicinal Plants Research, College of Life Sciences, South China Agricultural University, Guangzhou 510642, PR China; Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agricultural University, Guangzhou 510642, PR China; Corresponding author.Avian pathogenic Escherichia coli (APEC) is an important bacterial infectious disease can pose a threat to poultry and human health, with infected chicks typically exhibiting diarrhea and enteritis. Cinnamon essential oil (CEO) exhibits significant antibacterial, anti-inflammatory and intestinal regulatory properties but limited bioavailability. This study aims to develop an enteric CEO microcapsule and investigate its regulatory effects on anti-inflammatory, and intestinal microenvironment in E. coli-infected chickens, and to reveal the reasons for its superior therapeutic effect on CEO. Different concentrations of enteric CEO microcapsules and CEO were used to treat artificially infected chicken E. coli models, respectively. Cecal content was collected for 16S rRNA gene sequence analysis and metabonomics. Butyric acid content was detected by gas chromatography-flame ionization detection (GC-FID). Inflammatory factors and lipopolysaccharides (LPS) of serum were measured by corresponding ELISA kits. And inflammatory factors in ileum, jejunum and duodenum tissues were detected via quantitative real-time PCR (qRT‒PCR). Pathologic changes in hepatocytes and duodenum were detected by H&E. Dissolution of enteric CEO microcapsules was determined by the paddle method. We observed that the low-dose CEO microcapsule (APEC+MCL) with at least 75 % lower CEO content increased the bioavailability of CEO. This effect was associated with an improvement in the internal environment of the intestinal tract, as in increasing beneficial bacteria and reducing harmful bacteria. Meanwhile, APEC+MCL significantly increased butyric acid content and restored the gut metabolites, improved the level of intestinal inflammatory factors and reduced the inflammatory response, particularly in the duodenum and jejunum segments, with a significant increase in therapeutic effect. Furthermore, APEC+MCL also improved the levels of TNF-α, IL-1β, IL-6, and IL-10 in serum, as well as the liver's protective effect to reduce mortality and increase feed conversion rate (FCR) and average daily gain (ADG). These findings suggest enteric microcapsule system for CEO significantly enhances CEO's therapeutic potential, offering a promising strategy for developing more efficient and cost-effective natural antibacterial treatments for colibacillosis.http://www.sciencedirect.com/science/article/pii/S0032579125008508Chicken colibacillosisEnteric CEO microcapsuleInflammatoryIntestinal microfloraGut metabolites |
| spellingShingle | Huanling Xing Fang Zhou Jianhao Lin Junfeng Ban Junyi Huang Xinghai Li Hanjun He Yanqun Li Mei Bai Xiangxiu Liang Hong Wu Enteric-coated microencapsulation improves the therapeutic effect of cinnamon oil on artificially infected chicken with Escherichia coli Poultry Science Chicken colibacillosis Enteric CEO microcapsule Inflammatory Intestinal microflora Gut metabolites |
| title | Enteric-coated microencapsulation improves the therapeutic effect of cinnamon oil on artificially infected chicken with Escherichia coli |
| title_full | Enteric-coated microencapsulation improves the therapeutic effect of cinnamon oil on artificially infected chicken with Escherichia coli |
| title_fullStr | Enteric-coated microencapsulation improves the therapeutic effect of cinnamon oil on artificially infected chicken with Escherichia coli |
| title_full_unstemmed | Enteric-coated microencapsulation improves the therapeutic effect of cinnamon oil on artificially infected chicken with Escherichia coli |
| title_short | Enteric-coated microencapsulation improves the therapeutic effect of cinnamon oil on artificially infected chicken with Escherichia coli |
| title_sort | enteric coated microencapsulation improves the therapeutic effect of cinnamon oil on artificially infected chicken with escherichia coli |
| topic | Chicken colibacillosis Enteric CEO microcapsule Inflammatory Intestinal microflora Gut metabolites |
| url | http://www.sciencedirect.com/science/article/pii/S0032579125008508 |
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