Antagonistic effect of Pseudomonas aeruginosa on Candida auris
IntroductionCandida (Candidozyma) auris and Pseudomonas aeruginosa are frequently found in hospital environments and on medical equipment, where they commonly colonize and infect hospitalized patients, contributing to healthcare-associated infections (HAIs). Although they share similar ecological ni...
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Frontiers Media S.A.
2025-07-01
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| author | Ana Beatriz N. Macedo Daniele de Figuerêdo Silva Anthony G. J. Medeiros Gustavo José Freitas Murilo Moreira dos Santos Kelly Ishida Nalu Teixeira de Aguiar Peres Nalu Teixeira de Aguiar Peres Daniel Assis Santos Daniel Assis Santos Luana Rossato Gustavo H. Goldman Gustavo H. Goldman Rafael Wesley Bastos Rafael Wesley Bastos |
| author_facet | Ana Beatriz N. Macedo Daniele de Figuerêdo Silva Anthony G. J. Medeiros Gustavo José Freitas Murilo Moreira dos Santos Kelly Ishida Nalu Teixeira de Aguiar Peres Nalu Teixeira de Aguiar Peres Daniel Assis Santos Daniel Assis Santos Luana Rossato Gustavo H. Goldman Gustavo H. Goldman Rafael Wesley Bastos Rafael Wesley Bastos |
| author_sort | Ana Beatriz N. Macedo |
| collection | DOAJ |
| description | IntroductionCandida (Candidozyma) auris and Pseudomonas aeruginosa are frequently found in hospital environments and on medical equipment, where they commonly colonize and infect hospitalized patients, contributing to healthcare-associated infections (HAIs). Although they share similar ecological niches and may interact, the mechanisms underlying their interspecies communication remain largely unknown.MethodsThis study investigated the in vitro interaction between planktonic cells of C. auris and P. aeruginosa through co-culture experiments in various growth media, with or without iron supplementation. Fluorescence microscopy was employed to assess yeast viability, and the effect of lyophilized, cell-free P. aeruginosa supernatants on C. auris was also evaluated.ResultsP. aeruginosa significantly inhibited the growth of C. auris, regardless of the initial microbial concentrations. Growth suppression began after 8 hours of co-culture and persisted for up to 72 hours. Fluorescence microscopy suggested that this antagonistic effect was predominantly fungistatic, as most C. auris cells remained viable in the presence of the bacterium. The inhibitory effect was consistent across different culture media, and iron supplementation partially restored C. auris growth. Similarly, concentrated cell-free supernatants from P. aeruginosa inhibited C. auris, further supporting the role of secreted molecules. In this case as well, iron addition partially reversed the inhibitory effect.Discussion and conclusionThese findings suggest that P. aeruginosa produces and secretes molecules with fungistatic activity against C. auris, and that this effect is at least partially modulated by iron availability. This discovery provides a foundation for future research into the identity and mechanisms of action of these secreted compounds, as well as the broader clinical implications of microbial interactions during co-colonization or co-infection. |
| format | Article |
| id | doaj-art-6bbd3ed2958c4aa7ae19b7fe43a0b219 |
| institution | Matheson Library |
| issn | 2673-6128 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Fungal Biology |
| spelling | doaj-art-6bbd3ed2958c4aa7ae19b7fe43a0b2192025-07-10T08:57:29ZengFrontiers Media S.A.Frontiers in Fungal Biology2673-61282025-07-01610.3389/ffunb.2025.16132441613244Antagonistic effect of Pseudomonas aeruginosa on Candida aurisAna Beatriz N. Macedo0Daniele de Figuerêdo Silva1Anthony G. J. Medeiros2Gustavo José Freitas3Murilo Moreira dos Santos4Kelly Ishida5Nalu Teixeira de Aguiar Peres6Nalu Teixeira de Aguiar Peres7Daniel Assis Santos8Daniel Assis Santos9Luana Rossato10Gustavo H. Goldman11Gustavo H. Goldman12Rafael Wesley Bastos13Rafael Wesley Bastos14Departamento de Microbiologia e Parasitologia, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, BrazilDepartamento de Microbiologia e Parasitologia, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, BrazilDepartamento de Microbiologia e Parasitologia, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, BrazilDepartamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, BrazilDepartamento de Microbiologia, Universidade de São Paulo, São Paulo, BrazilDepartamento de Microbiologia, Universidade de São Paulo, São Paulo, BrazilDepartamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, BrazilInstituto Nacional de Ciência e Tecnologia, Ribeirão Preto, São Paulo, BrazilDepartamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, BrazilInstituto Nacional de Ciência e Tecnologia, Ribeirão Preto, São Paulo, BrazilLaboratório de Pesquisa em Ciências da Saúde, Universidade Federal da Grande Dourados, Dourados, Mato Grosso do Sul, BrazilInstituto Nacional de Ciência e Tecnologia, Ribeirão Preto, São Paulo, BrazilFaculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, BrazilDepartamento de Microbiologia e Parasitologia, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, BrazilInstituto Nacional de Ciência e Tecnologia, Ribeirão Preto, São Paulo, BrazilIntroductionCandida (Candidozyma) auris and Pseudomonas aeruginosa are frequently found in hospital environments and on medical equipment, where they commonly colonize and infect hospitalized patients, contributing to healthcare-associated infections (HAIs). Although they share similar ecological niches and may interact, the mechanisms underlying their interspecies communication remain largely unknown.MethodsThis study investigated the in vitro interaction between planktonic cells of C. auris and P. aeruginosa through co-culture experiments in various growth media, with or without iron supplementation. Fluorescence microscopy was employed to assess yeast viability, and the effect of lyophilized, cell-free P. aeruginosa supernatants on C. auris was also evaluated.ResultsP. aeruginosa significantly inhibited the growth of C. auris, regardless of the initial microbial concentrations. Growth suppression began after 8 hours of co-culture and persisted for up to 72 hours. Fluorescence microscopy suggested that this antagonistic effect was predominantly fungistatic, as most C. auris cells remained viable in the presence of the bacterium. The inhibitory effect was consistent across different culture media, and iron supplementation partially restored C. auris growth. Similarly, concentrated cell-free supernatants from P. aeruginosa inhibited C. auris, further supporting the role of secreted molecules. In this case as well, iron addition partially reversed the inhibitory effect.Discussion and conclusionThese findings suggest that P. aeruginosa produces and secretes molecules with fungistatic activity against C. auris, and that this effect is at least partially modulated by iron availability. This discovery provides a foundation for future research into the identity and mechanisms of action of these secreted compounds, as well as the broader clinical implications of microbial interactions during co-colonization or co-infection.https://www.frontiersin.org/articles/10.3389/ffunb.2025.1613244/fullmicrobial interactionhealthcare-associated infectionsfungistatic effectironcoculture |
| spellingShingle | Ana Beatriz N. Macedo Daniele de Figuerêdo Silva Anthony G. J. Medeiros Gustavo José Freitas Murilo Moreira dos Santos Kelly Ishida Nalu Teixeira de Aguiar Peres Nalu Teixeira de Aguiar Peres Daniel Assis Santos Daniel Assis Santos Luana Rossato Gustavo H. Goldman Gustavo H. Goldman Rafael Wesley Bastos Rafael Wesley Bastos Antagonistic effect of Pseudomonas aeruginosa on Candida auris Frontiers in Fungal Biology microbial interaction healthcare-associated infections fungistatic effect iron coculture |
| title | Antagonistic effect of Pseudomonas aeruginosa on Candida auris |
| title_full | Antagonistic effect of Pseudomonas aeruginosa on Candida auris |
| title_fullStr | Antagonistic effect of Pseudomonas aeruginosa on Candida auris |
| title_full_unstemmed | Antagonistic effect of Pseudomonas aeruginosa on Candida auris |
| title_short | Antagonistic effect of Pseudomonas aeruginosa on Candida auris |
| title_sort | antagonistic effect of pseudomonas aeruginosa on candida auris |
| topic | microbial interaction healthcare-associated infections fungistatic effect iron coculture |
| url | https://www.frontiersin.org/articles/10.3389/ffunb.2025.1613244/full |
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