Unveiling anti-inflammatory peptides from Lion’s Mane mushroom (Hericium erinaceus): Preparation, bioactivity assessment, and peptides identification
Lion’s Mane mushroom (Hericium erinaceus) is acknowledged worldwide for its substantial contribution of medicinal compounds and nutrients, including protein. The efficient extraction and hydrolysis of proteins are essential for revealing their bioactive properties. This study demonstrates that the o...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-12-01
|
| Series: | Applied Food Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S277250222500472X |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1839630459460386816 |
|---|---|
| author | Buddhika Silva Korawan Sringarm Saranyapin Potikanond Pipat Tangjaidee Pensiri Buacheen Pornchai Rachtanapun Natthawuddhi Donlao Jaspreet Singh Lovedeep Kaur Utthapon Issara Passakorn Kingwascharapong Suphat Phongthai |
| author_facet | Buddhika Silva Korawan Sringarm Saranyapin Potikanond Pipat Tangjaidee Pensiri Buacheen Pornchai Rachtanapun Natthawuddhi Donlao Jaspreet Singh Lovedeep Kaur Utthapon Issara Passakorn Kingwascharapong Suphat Phongthai |
| author_sort | Buddhika Silva |
| collection | DOAJ |
| description | Lion’s Mane mushroom (Hericium erinaceus) is acknowledged worldwide for its substantial contribution of medicinal compounds and nutrients, including protein. The efficient extraction and hydrolysis of proteins are essential for revealing their bioactive properties. This study demonstrates that the optimal pulsed electric field (PEF)-assisted extraction achieved a 42.44 % increase in protein extraction efficiency relative to traditional alkaline extraction (p < 0.05). H. erinaceus protein contained 44.59 % essential amino acids and exhibited 71.33 % in vitro digestibility. Pepsin-trypsin hydrolysis produced the most significant anti-inflammatory activity, resulting in a 36.2 % reduction in nitric oxide and a 31.8 % decrease in interleukin-6 levels (p < 0.05). Subsequent fractionations employing membrane ultrafiltration and size exclusion chromatography effectively purified the peptides, resulting in enhanced anti-inflammatory activity (p < 0.05). This research discovered nine important peptide sequences containing 50–100 % hydrophobic amino acids in Lion's Mane mushroom proteins, which could aid in the synthesis of natural anti-inflammatory peptides. |
| format | Article |
| id | doaj-art-6afcf59a33664cdaad88d5f23e01b6a0 |
| institution | Matheson Library |
| issn | 2772-5022 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Applied Food Research |
| spelling | doaj-art-6afcf59a33664cdaad88d5f23e01b6a02025-07-14T04:15:28ZengElsevierApplied Food Research2772-50222025-12-0152101167Unveiling anti-inflammatory peptides from Lion’s Mane mushroom (Hericium erinaceus): Preparation, bioactivity assessment, and peptides identificationBuddhika Silva0Korawan Sringarm1Saranyapin Potikanond2Pipat Tangjaidee3Pensiri Buacheen4Pornchai Rachtanapun5Natthawuddhi Donlao6Jaspreet Singh7Lovedeep Kaur8Utthapon Issara9Passakorn Kingwascharapong10Suphat Phongthai11Program in Food Science and Technology (International Program), School of Agro-Industry, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, ThailandCenter of Excellence in Agro Bio-Circular-Green Industry (Agro BCG), Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, ThailandDepartment of Pharmacology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandProgram in Food Science and Technology (International Program), School of Agro-Industry, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, Thailand; Center of Excellence in Agro Bio-Circular-Green Industry (Agro BCG), Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, ThailandDepartment of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandProgram in Food Science and Technology (International Program), School of Agro-Industry, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, Thailand; Center of Excellence in Agro Bio-Circular-Green Industry (Agro BCG), Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, ThailandUnit of Innovative Food Packaging and Biomaterials School of Agro-Industry, Mae Fah Luang University, Chiang Rai 57100, ThailandSchool of Food and Advanced Technology, Massey University, Palmerston North 4442, New ZealandSchool of Food and Advanced Technology, Massey University, Palmerston North 4442, New ZealandDivision of Food Science and Technology Management, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Pathum Thani 12110, ThailandDepartment of Fishery Products, Faculty of Fisheries, Kasetsart University, Bangkok 10900, ThailandProgram in Food Science and Technology (International Program), School of Agro-Industry, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, Thailand; Center of Excellence in Agro Bio-Circular-Green Industry (Agro BCG), Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, Thailand; Corresponding author at: Program in Food Science and Technology (International Program), School of Agro-Industry, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, Thailand.Lion’s Mane mushroom (Hericium erinaceus) is acknowledged worldwide for its substantial contribution of medicinal compounds and nutrients, including protein. The efficient extraction and hydrolysis of proteins are essential for revealing their bioactive properties. This study demonstrates that the optimal pulsed electric field (PEF)-assisted extraction achieved a 42.44 % increase in protein extraction efficiency relative to traditional alkaline extraction (p < 0.05). H. erinaceus protein contained 44.59 % essential amino acids and exhibited 71.33 % in vitro digestibility. Pepsin-trypsin hydrolysis produced the most significant anti-inflammatory activity, resulting in a 36.2 % reduction in nitric oxide and a 31.8 % decrease in interleukin-6 levels (p < 0.05). Subsequent fractionations employing membrane ultrafiltration and size exclusion chromatography effectively purified the peptides, resulting in enhanced anti-inflammatory activity (p < 0.05). This research discovered nine important peptide sequences containing 50–100 % hydrophobic amino acids in Lion's Mane mushroom proteins, which could aid in the synthesis of natural anti-inflammatory peptides.http://www.sciencedirect.com/science/article/pii/S277250222500472XAnti-inflammatory peptideAlternative proteinsLion’s Mane mushroom (Hericium erinaceus)Protein hydrolysatesIn vitro digestion |
| spellingShingle | Buddhika Silva Korawan Sringarm Saranyapin Potikanond Pipat Tangjaidee Pensiri Buacheen Pornchai Rachtanapun Natthawuddhi Donlao Jaspreet Singh Lovedeep Kaur Utthapon Issara Passakorn Kingwascharapong Suphat Phongthai Unveiling anti-inflammatory peptides from Lion’s Mane mushroom (Hericium erinaceus): Preparation, bioactivity assessment, and peptides identification Applied Food Research Anti-inflammatory peptide Alternative proteins Lion’s Mane mushroom (Hericium erinaceus) Protein hydrolysates In vitro digestion |
| title | Unveiling anti-inflammatory peptides from Lion’s Mane mushroom (Hericium erinaceus): Preparation, bioactivity assessment, and peptides identification |
| title_full | Unveiling anti-inflammatory peptides from Lion’s Mane mushroom (Hericium erinaceus): Preparation, bioactivity assessment, and peptides identification |
| title_fullStr | Unveiling anti-inflammatory peptides from Lion’s Mane mushroom (Hericium erinaceus): Preparation, bioactivity assessment, and peptides identification |
| title_full_unstemmed | Unveiling anti-inflammatory peptides from Lion’s Mane mushroom (Hericium erinaceus): Preparation, bioactivity assessment, and peptides identification |
| title_short | Unveiling anti-inflammatory peptides from Lion’s Mane mushroom (Hericium erinaceus): Preparation, bioactivity assessment, and peptides identification |
| title_sort | unveiling anti inflammatory peptides from lion s mane mushroom hericium erinaceus preparation bioactivity assessment and peptides identification |
| topic | Anti-inflammatory peptide Alternative proteins Lion’s Mane mushroom (Hericium erinaceus) Protein hydrolysates In vitro digestion |
| url | http://www.sciencedirect.com/science/article/pii/S277250222500472X |
| work_keys_str_mv | AT buddhikasilva unveilingantiinflammatorypeptidesfromlionsmanemushroomhericiumerinaceuspreparationbioactivityassessmentandpeptidesidentification AT korawansringarm unveilingantiinflammatorypeptidesfromlionsmanemushroomhericiumerinaceuspreparationbioactivityassessmentandpeptidesidentification AT saranyapinpotikanond unveilingantiinflammatorypeptidesfromlionsmanemushroomhericiumerinaceuspreparationbioactivityassessmentandpeptidesidentification AT pipattangjaidee unveilingantiinflammatorypeptidesfromlionsmanemushroomhericiumerinaceuspreparationbioactivityassessmentandpeptidesidentification AT pensiribuacheen unveilingantiinflammatorypeptidesfromlionsmanemushroomhericiumerinaceuspreparationbioactivityassessmentandpeptidesidentification AT pornchairachtanapun unveilingantiinflammatorypeptidesfromlionsmanemushroomhericiumerinaceuspreparationbioactivityassessmentandpeptidesidentification AT natthawuddhidonlao unveilingantiinflammatorypeptidesfromlionsmanemushroomhericiumerinaceuspreparationbioactivityassessmentandpeptidesidentification AT jaspreetsingh unveilingantiinflammatorypeptidesfromlionsmanemushroomhericiumerinaceuspreparationbioactivityassessmentandpeptidesidentification AT lovedeepkaur unveilingantiinflammatorypeptidesfromlionsmanemushroomhericiumerinaceuspreparationbioactivityassessmentandpeptidesidentification AT utthaponissara unveilingantiinflammatorypeptidesfromlionsmanemushroomhericiumerinaceuspreparationbioactivityassessmentandpeptidesidentification AT passakornkingwascharapong unveilingantiinflammatorypeptidesfromlionsmanemushroomhericiumerinaceuspreparationbioactivityassessmentandpeptidesidentification AT suphatphongthai unveilingantiinflammatorypeptidesfromlionsmanemushroomhericiumerinaceuspreparationbioactivityassessmentandpeptidesidentification |