Effect of ergothioneine on tissue metabolism in middle-aged and aged mice
Objective To investigate the effect of ergothioneine(EGT) on tissue metabolism in middle-aged and aged mice. Methods Nine-month-old middle-aged and aged C57BL/6J mice were selected to be gavaged with EGT aqueous solution at the dose and frequency of 35 mg EGT per kg body weight every two days. The c...
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2025-06-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | https://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2025-45-6-709.pdf |
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| author | CHEN Haoran, WANG Fang, MA Yanni, YU Jia, WANG Lin |
| author_facet | CHEN Haoran, WANG Fang, MA Yanni, YU Jia, WANG Lin |
| author_sort | CHEN Haoran, WANG Fang, MA Yanni, YU Jia, WANG Lin |
| collection | DOAJ |
| description | Objective To investigate the effect of ergothioneine(EGT) on tissue metabolism in middle-aged and aged mice. Methods Nine-month-old middle-aged and aged C57BL/6J mice were selected to be gavaged with EGT aqueous solution at the dose and frequency of 35 mg EGT per kg body weight every two days. The control group was gavaged with the same amount of water. Both groups were fed with EGT-free diet. After 7 weeks, the liver, kidney, and small intestine of mice were collected, and untargeted metabolomics analysis was performed using ultra-performance liquid chromatography-mass spectrometry(UHPLC-MS). The differential metabolites were selected for KEGG metabolic pathway enrichment analysis. Results Compared with the control group, the abundances of metabolites related to redox balance in liver, kidney and small intestine of middle-aged and aged mice treated with EGT did not change significantly. However, taurine and hypotaurine metabolism in liver(P＜0.01), purine metabolism in kidney(P＜0.01), and cysteine and methionine metabolism in small intestine(P＜0.001) were affected. Conclusions Non-redox-related metabolic changes in the liver, kidney and small intestine of middle-aged and aged mice by ergothioheine. |
| format | Article |
| id | doaj-art-6ab56484b0194e45b9fd1e2d9facafb3 |
| institution | Matheson Library |
| issn | 1001-6325 |
| language | zho |
| publishDate | 2025-06-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj-art-6ab56484b0194e45b9fd1e2d9facafb32025-07-01T06:27:55ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252025-06-0145670971310.16352/j.issn.1001-6325.2025.06.0709Effect of ergothioneine on tissue metabolism in middle-aged and aged miceCHEN Haoran, WANG Fang, MA Yanni, YU Jia, WANG Lin0State Key Laboratory of Common Mechanism Research for Major Diseases, Department of Biochemistry and Molecular Biology, Institute of Basic Medicine CAMS, School of Basic Medicine PUMC, Beijing 100005, ChinaObjective To investigate the effect of ergothioneine(EGT) on tissue metabolism in middle-aged and aged mice. Methods Nine-month-old middle-aged and aged C57BL/6J mice were selected to be gavaged with EGT aqueous solution at the dose and frequency of 35 mg EGT per kg body weight every two days. The control group was gavaged with the same amount of water. Both groups were fed with EGT-free diet. After 7 weeks, the liver, kidney, and small intestine of mice were collected, and untargeted metabolomics analysis was performed using ultra-performance liquid chromatography-mass spectrometry(UHPLC-MS). The differential metabolites were selected for KEGG metabolic pathway enrichment analysis. Results Compared with the control group, the abundances of metabolites related to redox balance in liver, kidney and small intestine of middle-aged and aged mice treated with EGT did not change significantly. However, taurine and hypotaurine metabolism in liver(P＜0.01), purine metabolism in kidney(P＜0.01), and cysteine and methionine metabolism in small intestine(P＜0.001) were affected. Conclusions Non-redox-related metabolic changes in the liver, kidney and small intestine of middle-aged and aged mice by ergothioheine.https://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2025-45-6-709.pdfergothioneine|middle-aged and aged mice|metabolome|metabolic pathways |
| spellingShingle | CHEN Haoran, WANG Fang, MA Yanni, YU Jia, WANG Lin Effect of ergothioneine on tissue metabolism in middle-aged and aged mice Jichu yixue yu linchuang ergothioneine|middle-aged and aged mice|metabolome|metabolic pathways |
| title | Effect of ergothioneine on tissue metabolism in middle-aged and aged mice |
| title_full | Effect of ergothioneine on tissue metabolism in middle-aged and aged mice |
| title_fullStr | Effect of ergothioneine on tissue metabolism in middle-aged and aged mice |
| title_full_unstemmed | Effect of ergothioneine on tissue metabolism in middle-aged and aged mice |
| title_short | Effect of ergothioneine on tissue metabolism in middle-aged and aged mice |
| title_sort | effect of ergothioneine on tissue metabolism in middle aged and aged mice |
| topic | ergothioneine|middle-aged and aged mice|metabolome|metabolic pathways |
| url | https://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2025-45-6-709.pdf |
| work_keys_str_mv | AT chenhaoranwangfangmayanniyujiawanglin effectofergothioneineontissuemetabolisminmiddleagedandagedmice |