Electrical and viscoelastic parameters of erythrocytes as predictors of exacerbation in inflammatory bowel diseases
The aim of this work is to study the electrical and viscoelastic parameters of erythrocytes for patients with inflammatory bowel diseases (IBD), examined in dynamics, as possible predictors of exacerbation of the disease. Material and methods. In dynamics, 23 patients with IBD (37.9 ± 4.3 years) wer...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
Russian Academy of Sciences, Siberian Branch Publishing House
2021-10-01
|
| Series: | Сибирский научный медицинский журнал |
| Subjects: | |
| Online Access: | https://sibmed.elpub.ru/jour/article/view/669 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1839628772542775296 |
|---|---|
| author | M. V. Kruchinina I. O. Svetlova A. V. Azgaldyan A. A. Gromov V. M. Generalov I. N. Yakovina A. A. Shestov |
| author_facet | M. V. Kruchinina I. O. Svetlova A. V. Azgaldyan A. A. Gromov V. M. Generalov I. N. Yakovina A. A. Shestov |
| author_sort | M. V. Kruchinina |
| collection | DOAJ |
| description | The aim of this work is to study the electrical and viscoelastic parameters of erythrocytes for patients with inflammatory bowel diseases (IBD), examined in dynamics, as possible predictors of exacerbation of the disease. Material and methods. In dynamics, 23 patients with IBD (37.9 ± 4.3 years) were examined. The first examination was carried out in the stage of exacerbation, the second – after 6–12 months – in the stage of remission of IBD. Additionally, 36 patients with IBD in remission were examined to assess the predictive capabilities of erythrocyte parameters with subsequent monitoring of their condition for 6–12 months. The electrical and viscoelastic parameters of erythrocytes were investigated by dielectrophoresis. Statistical processing was carried out using a system of machine learning methods – Random Forest, MATLAB (R2019a, MathWorks). Results and discussion. The parameters of erythrocytes, which are predictors of exacerbation of IBD, were revealed: an increase in the proportion of deformed cells (p = 0.0001); altered nature of the erythrocyte surface (p = 0.011); increased electrical conductivity of membranes (p = 0.0019); reduced dipole moment (p = 0.0015), the rate of directional movement of erythrocytes to the electrodes (p = 0.005) (paired and unpaired Volcano plot methods), reduced deformation amplitude at a frequency of 5×105 Hz (p = 0.009), capacity of erythrocyte membranes (p = 0.029 ) (unpaired Volcano plot method). Indicators associated with changes in the charge of cells, the structure of erythrocyte membranes and the level of energy metabolism of the cell turned out to be significant. A high diagnostic accuracy of these indicators was established as predictors: the proportion of deformed cells (AUC 0.939), a summarized viscosity index (AUC 0.932). Cluster analysis of the values of erythrocyte parameters – predictors of exacerbation made it possible to stratify the risk of exacerbation: at the most altered levels of parameters – the development of exacerbation after 2.5–4.5 months, intermediate levels – after 4–6 months, minimally changed – after 8–12 months (predictive accuracy – 92 %). Conclusions. The revealed electrical and viscoelastic parameters of erythrocytes should be considered as promising predictors of exacerbation in patients with IBD. |
| format | Article |
| id | doaj-art-6aa5d7abdcab4919b9f3a9a1c2f8e9a2 |
| institution | Matheson Library |
| issn | 2410-2512 2410-2520 |
| language | Russian |
| publishDate | 2021-10-01 |
| publisher | Russian Academy of Sciences, Siberian Branch Publishing House |
| record_format | Article |
| series | Сибирский научный медицинский журнал |
| spelling | doaj-art-6aa5d7abdcab4919b9f3a9a1c2f8e9a22025-07-15T11:26:36ZrusRussian Academy of Sciences, Siberian Branch Publishing HouseСибирский научный медицинский журнал2410-25122410-25202021-10-014159611210.18699/SSMJ20210513386Electrical and viscoelastic parameters of erythrocytes as predictors of exacerbation in inflammatory bowel diseasesM. V. Kruchinina0I. O. Svetlova1A. V. Azgaldyan2A. A. Gromov3V. M. Generalov4I. N. Yakovina5A. A. Shestov6Research Institute of Internal and Preventive Medicine – Branch of the Institute of Cytology and Genetics SB RAS; Novosibirsk State Medical University of Minzdrav of RussiaResearch Institute of Internal and Preventive Medicine – Branch of the Institute of Cytology and Genetics SB RAS; Novosibirsk State Medical University of Minzdrav of RussiaResearch Institute of Internal and Preventive Medicine – Branch of the Institute of Cytology and Genetics SB RASResearch Institute of Internal and Preventive Medicine – Branch of the Institute of Cytology and Genetics SB RASState Research Center of Virology and Biotechnology «Vector» of RospotrebnadzorNovosibirsk State Technical UniversityUniversity of PennsylvaniaThe aim of this work is to study the electrical and viscoelastic parameters of erythrocytes for patients with inflammatory bowel diseases (IBD), examined in dynamics, as possible predictors of exacerbation of the disease. Material and methods. In dynamics, 23 patients with IBD (37.9 ± 4.3 years) were examined. The first examination was carried out in the stage of exacerbation, the second – after 6–12 months – in the stage of remission of IBD. Additionally, 36 patients with IBD in remission were examined to assess the predictive capabilities of erythrocyte parameters with subsequent monitoring of their condition for 6–12 months. The electrical and viscoelastic parameters of erythrocytes were investigated by dielectrophoresis. Statistical processing was carried out using a system of machine learning methods – Random Forest, MATLAB (R2019a, MathWorks). Results and discussion. The parameters of erythrocytes, which are predictors of exacerbation of IBD, were revealed: an increase in the proportion of deformed cells (p = 0.0001); altered nature of the erythrocyte surface (p = 0.011); increased electrical conductivity of membranes (p = 0.0019); reduced dipole moment (p = 0.0015), the rate of directional movement of erythrocytes to the electrodes (p = 0.005) (paired and unpaired Volcano plot methods), reduced deformation amplitude at a frequency of 5×105 Hz (p = 0.009), capacity of erythrocyte membranes (p = 0.029 ) (unpaired Volcano plot method). Indicators associated with changes in the charge of cells, the structure of erythrocyte membranes and the level of energy metabolism of the cell turned out to be significant. A high diagnostic accuracy of these indicators was established as predictors: the proportion of deformed cells (AUC 0.939), a summarized viscosity index (AUC 0.932). Cluster analysis of the values of erythrocyte parameters – predictors of exacerbation made it possible to stratify the risk of exacerbation: at the most altered levels of parameters – the development of exacerbation after 2.5–4.5 months, intermediate levels – after 4–6 months, minimally changed – after 8–12 months (predictive accuracy – 92 %). Conclusions. The revealed electrical and viscoelastic parameters of erythrocytes should be considered as promising predictors of exacerbation in patients with IBD.https://sibmed.elpub.ru/jour/article/view/669electricalviscoelastic parameters of erythrocytespredictorsexacerbationinflammatory bowel disease |
| spellingShingle | M. V. Kruchinina I. O. Svetlova A. V. Azgaldyan A. A. Gromov V. M. Generalov I. N. Yakovina A. A. Shestov Electrical and viscoelastic parameters of erythrocytes as predictors of exacerbation in inflammatory bowel diseases Сибирский научный медицинский журнал electrical viscoelastic parameters of erythrocytes predictors exacerbation inflammatory bowel disease |
| title | Electrical and viscoelastic parameters of erythrocytes as predictors of exacerbation in inflammatory bowel diseases |
| title_full | Electrical and viscoelastic parameters of erythrocytes as predictors of exacerbation in inflammatory bowel diseases |
| title_fullStr | Electrical and viscoelastic parameters of erythrocytes as predictors of exacerbation in inflammatory bowel diseases |
| title_full_unstemmed | Electrical and viscoelastic parameters of erythrocytes as predictors of exacerbation in inflammatory bowel diseases |
| title_short | Electrical and viscoelastic parameters of erythrocytes as predictors of exacerbation in inflammatory bowel diseases |
| title_sort | electrical and viscoelastic parameters of erythrocytes as predictors of exacerbation in inflammatory bowel diseases |
| topic | electrical viscoelastic parameters of erythrocytes predictors exacerbation inflammatory bowel disease |
| url | https://sibmed.elpub.ru/jour/article/view/669 |
| work_keys_str_mv | AT mvkruchinina electricalandviscoelasticparametersoferythrocytesaspredictorsofexacerbationininflammatoryboweldiseases AT iosvetlova electricalandviscoelasticparametersoferythrocytesaspredictorsofexacerbationininflammatoryboweldiseases AT avazgaldyan electricalandviscoelasticparametersoferythrocytesaspredictorsofexacerbationininflammatoryboweldiseases AT aagromov electricalandviscoelasticparametersoferythrocytesaspredictorsofexacerbationininflammatoryboweldiseases AT vmgeneralov electricalandviscoelasticparametersoferythrocytesaspredictorsofexacerbationininflammatoryboweldiseases AT inyakovina electricalandviscoelasticparametersoferythrocytesaspredictorsofexacerbationininflammatoryboweldiseases AT aashestov electricalandviscoelasticparametersoferythrocytesaspredictorsofexacerbationininflammatoryboweldiseases |