The Role of Serum Expression Levels of Microrna-21 on Bone Mineral Density in Hypostrogenic Postmenopausal Women with Osteoporosis: Study on Level of RANKL, OPG, TGFβ-1, Sclerostin, RANKL/OPG Ratio, and Physical Activity
Background: MiR-21 is known to play a role in osteoclast proliferation and differentiation, but the role of serum miR-21 expression in osteoporosis remains unclear. Previous research found that serum miR-21 expression was positively correlated with bone mineral density in postmenopausal osteoporosis...
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| Format: | Article |
| Language: | English |
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Interna Publishing
2019-11-01
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| Series: | Acta Medica Indonesiana |
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| Online Access: | https://www.actamedindones.org/index.php/ijim/article/view/808 |
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| author | I Nyoman Suarjana Harry Isbagio Pradana Soewondo Ichramsjah A Rachman Mohammad Sadikin Joedo Prihartono Safarina G Malik Joewono Soeroso |
| author_facet | I Nyoman Suarjana Harry Isbagio Pradana Soewondo Ichramsjah A Rachman Mohammad Sadikin Joedo Prihartono Safarina G Malik Joewono Soeroso |
| author_sort | I Nyoman Suarjana |
| collection | DOAJ |
| description | Background: MiR-21 is known to play a role in osteoclast proliferation and differentiation, but the role of serum miR-21 expression in osteoporosis remains unclear. Previous research found that serum miR-21 expression was positively correlated with bone mineral density in postmenopausal osteoporosis patients, but other factors involved in postmenopausal osteoporosis still unknown. This study aimed to determine the role of serum miR-21 expression, concentration of RANKL, OPG, TGF-β1, sclerostin and serum calcium, RANKL/OPG ratio, and physical activity on bone mineral density of spine in hypoestrogenic postmenopausal women with osteoporosis (PMOP) compared with no osteoporosis (PMNOP), with point of interest on the expression of serum miR-21. Methods: this study was conducted by comparative cross-sectional design. The subjects were divided into 2 groups of PMOP and PMNOP. We used an absolute quantification real-time PCR method to determine serum miR-21 expressions level. Results: Median of serum miR-21 expression at the PMOP group was significantly higher compared to PMNOP group (p = 0.001). Serum miR-21 expression, RANKL, RANKL/OPG ratio, and physical activity were significantly correlated with BMD values in the PMOP group. Moderate physical activity was significantly negatively correlated with serum miR-21 expression. We also obtained a linear regression equation BMD = 1.373-0.085*Ln.miR-21-0.176*Log10.RANKL (R2 = 52.5%). Conclusion: serum miR-21 expression in PMOP was higher compared with PMNOP. Serum miR-21 expression proved to have a negative effect on spinal BMD values in hypoestrogenic postmenopausal women with osteoporosis of 8.5%. Obtained equation of BMD = 1.373-0.085*Ln.miR-21-0.176*Log10.RANKL can explain the value of spinal BMD by 52.5%.
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| format | Article |
| id | doaj-art-6a30c1aff38245b2be72e82d362cb15c |
| institution | Matheson Library |
| issn | 0125-9326 2338-2732 |
| language | English |
| publishDate | 2019-11-01 |
| publisher | Interna Publishing |
| record_format | Article |
| series | Acta Medica Indonesiana |
| spelling | doaj-art-6a30c1aff38245b2be72e82d362cb15c2025-07-23T07:01:46ZengInterna PublishingActa Medica Indonesiana0125-93262338-27322019-11-01513The Role of Serum Expression Levels of Microrna-21 on Bone Mineral Density in Hypostrogenic Postmenopausal Women with Osteoporosis: Study on Level of RANKL, OPG, TGFβ-1, Sclerostin, RANKL/OPG Ratio, and Physical ActivityI Nyoman Suarjana0Harry Isbagio1Pradana Soewondo2Ichramsjah A Rachman3Mohammad Sadikin4Joedo Prihartono5Safarina G Malik6Joewono Soeroso7Departement of Internal Medicine, Faculty of Medicine, University of Lambung Mangkurat, Banjarmasin, South KalimantanDepartement of Internal Medicine, Faculty of Medicine Universitas Indonesia, Jakarta, IndonesiaDepartement of Internal Medicine, Faculty of Medicine Universitas Indonesia, Jakarta, IndonesiaDepartement of Obstetrics and Gynecology, Faculty of Medicine, University of Indonesia, Jakarta, IndonesiaDepartement of Biochemistry and Molecular Biology, Faculty of Medicine Universitas Indonesia, Jakarta, IndonesiaDepartement of Community Medical Science, Faculty of Medicine Universitas Indonesia, Jakarta, IndonesiaEijkman Institute for Molecular Biology, Jakarta, IndonesiaDepartement of Internal Medicine, Faculty of Medicine, University of Airlangga, Surabaya, IndonesiaBackground: MiR-21 is known to play a role in osteoclast proliferation and differentiation, but the role of serum miR-21 expression in osteoporosis remains unclear. Previous research found that serum miR-21 expression was positively correlated with bone mineral density in postmenopausal osteoporosis patients, but other factors involved in postmenopausal osteoporosis still unknown. This study aimed to determine the role of serum miR-21 expression, concentration of RANKL, OPG, TGF-β1, sclerostin and serum calcium, RANKL/OPG ratio, and physical activity on bone mineral density of spine in hypoestrogenic postmenopausal women with osteoporosis (PMOP) compared with no osteoporosis (PMNOP), with point of interest on the expression of serum miR-21. Methods: this study was conducted by comparative cross-sectional design. The subjects were divided into 2 groups of PMOP and PMNOP. We used an absolute quantification real-time PCR method to determine serum miR-21 expressions level. Results: Median of serum miR-21 expression at the PMOP group was significantly higher compared to PMNOP group (p = 0.001). Serum miR-21 expression, RANKL, RANKL/OPG ratio, and physical activity were significantly correlated with BMD values in the PMOP group. Moderate physical activity was significantly negatively correlated with serum miR-21 expression. We also obtained a linear regression equation BMD = 1.373-0.085*Ln.miR-21-0.176*Log10.RANKL (R2 = 52.5%). Conclusion: serum miR-21 expression in PMOP was higher compared with PMNOP. Serum miR-21 expression proved to have a negative effect on spinal BMD values in hypoestrogenic postmenopausal women with osteoporosis of 8.5%. Obtained equation of BMD = 1.373-0.085*Ln.miR-21-0.176*Log10.RANKL can explain the value of spinal BMD by 52.5%. https://www.actamedindones.org/index.php/ijim/article/view/808BMDosteoporosisserum miR-21 |
| spellingShingle | I Nyoman Suarjana Harry Isbagio Pradana Soewondo Ichramsjah A Rachman Mohammad Sadikin Joedo Prihartono Safarina G Malik Joewono Soeroso The Role of Serum Expression Levels of Microrna-21 on Bone Mineral Density in Hypostrogenic Postmenopausal Women with Osteoporosis: Study on Level of RANKL, OPG, TGFβ-1, Sclerostin, RANKL/OPG Ratio, and Physical Activity Acta Medica Indonesiana BMD osteoporosis serum miR-21 |
| title | The Role of Serum Expression Levels of Microrna-21 on Bone Mineral Density in Hypostrogenic Postmenopausal Women with Osteoporosis: Study on Level of RANKL, OPG, TGFβ-1, Sclerostin, RANKL/OPG Ratio, and Physical Activity |
| title_full | The Role of Serum Expression Levels of Microrna-21 on Bone Mineral Density in Hypostrogenic Postmenopausal Women with Osteoporosis: Study on Level of RANKL, OPG, TGFβ-1, Sclerostin, RANKL/OPG Ratio, and Physical Activity |
| title_fullStr | The Role of Serum Expression Levels of Microrna-21 on Bone Mineral Density in Hypostrogenic Postmenopausal Women with Osteoporosis: Study on Level of RANKL, OPG, TGFβ-1, Sclerostin, RANKL/OPG Ratio, and Physical Activity |
| title_full_unstemmed | The Role of Serum Expression Levels of Microrna-21 on Bone Mineral Density in Hypostrogenic Postmenopausal Women with Osteoporosis: Study on Level of RANKL, OPG, TGFβ-1, Sclerostin, RANKL/OPG Ratio, and Physical Activity |
| title_short | The Role of Serum Expression Levels of Microrna-21 on Bone Mineral Density in Hypostrogenic Postmenopausal Women with Osteoporosis: Study on Level of RANKL, OPG, TGFβ-1, Sclerostin, RANKL/OPG Ratio, and Physical Activity |
| title_sort | role of serum expression levels of microrna 21 on bone mineral density in hypostrogenic postmenopausal women with osteoporosis study on level of rankl opg tgfβ 1 sclerostin rankl opg ratio and physical activity |
| topic | BMD osteoporosis serum miR-21 |
| url | https://www.actamedindones.org/index.php/ijim/article/view/808 |
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