Impact of sleep deprivation on creatine metabolism biomarkers in healthy young males

Background Creatine is a crucial component of cellular energy metabolism, facilitating ATP regeneration to support high energy demands. While its therapeutic potential, particularly in neuroprotection, is well-recognized, its response to stress conditions such as sleep deprivation remains insufficie...

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Main Authors: Nikola Todorovic, David Nedeljkovic, Jovana Panic, Sergej M. Ostojic
Format: Article
Sprog:engelsk
Udgivet: Taylor & Francis Group 2025-09-01
Serier:Journal of the International Society of Sports Nutrition
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Online adgang:https://www.tandfonline.com/doi/10.1080/15502783.2025.2533643
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author Nikola Todorovic
David Nedeljkovic
Jovana Panic
Sergej M. Ostojic
author_facet Nikola Todorovic
David Nedeljkovic
Jovana Panic
Sergej M. Ostojic
author_sort Nikola Todorovic
collection DOAJ
description Background Creatine is a crucial component of cellular energy metabolism, facilitating ATP regeneration to support high energy demands. While its therapeutic potential, particularly in neuroprotection, is well-recognized, its response to stress conditions such as sleep deprivation remains insufficiently investigated. This study examines the effects of sleep deprivation on creatine metabolism in healthy young males.Methods Sixteen healthy young male participants (age: 25.3 ± 4.7 years; body mass: 81.6 ± 8.8 kg) were recruited to assess the impact of 24-hour sleep deprivation on biomarkers of creatine metabolism. Primary outcomes included serum levels of creatine, guanidinoacetic acid (GAA), and creatinine, measured at baseline and after 24 hours.Results Sleep deprivation significantly increased serum creatine levels, rising from 48.63 ± 27.05 μmol/L at baseline to 63.84 ± 23.83 μmol/L after 24 hours (95% CI: −29.14 to −1.27; P = 0.03), with a moderate to large effect size (Cohen’s d = 0.59). Serum GAA levels showed a non-significant increase, from 3.27 ± 0.60 μmol/L to 3.47 ± 0.64 μmol/L (95% CI: −0.58 to 0.16; P = 0.26; Cohen’s d = 0.32). Serum creatinine levels remained unchanged, with no significant difference between baseline (100.57 ± 9.19 μmol/L) and follow-up (106.10 ± 27.90 μmol/L; 95% CI: −21.24 to 10.20; P = 0.46; Cohen’s d = 0.26).Conclusion Sleep deprivation alters creatine metabolism biomarkers, indicating shifts in metabolic pathways. Further research is necessary to validate and expand these findings, potentially elucidating the role of creatine in stress-related conditions.
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spelling doaj-art-690bae6995844aa79cb2acf94a0dfd212025-07-26T07:13:16ZengTaylor & Francis GroupJournal of the International Society of Sports Nutrition1550-27832025-09-0122sup110.1080/15502783.2025.2533643Impact of sleep deprivation on creatine metabolism biomarkers in healthy young malesNikola Todorovic0David Nedeljkovic1Jovana Panic2Sergej M. Ostojic3University of Novi Sad, Applied Bioenergetics Lab, Faculty of Sport and Physical Education, Novi Sad, SerbiaUniversity of Novi Sad, Applied Bioenergetics Lab, Faculty of Sport and Physical Education, Novi Sad, SerbiaUniversity of Novi Sad, Faculty of Sciences, Novi Sad, SerbiaUniversity of Novi Sad, Applied Bioenergetics Lab, Faculty of Sport and Physical Education, Novi Sad, SerbiaBackground Creatine is a crucial component of cellular energy metabolism, facilitating ATP regeneration to support high energy demands. While its therapeutic potential, particularly in neuroprotection, is well-recognized, its response to stress conditions such as sleep deprivation remains insufficiently investigated. This study examines the effects of sleep deprivation on creatine metabolism in healthy young males.Methods Sixteen healthy young male participants (age: 25.3 ± 4.7 years; body mass: 81.6 ± 8.8 kg) were recruited to assess the impact of 24-hour sleep deprivation on biomarkers of creatine metabolism. Primary outcomes included serum levels of creatine, guanidinoacetic acid (GAA), and creatinine, measured at baseline and after 24 hours.Results Sleep deprivation significantly increased serum creatine levels, rising from 48.63 ± 27.05 μmol/L at baseline to 63.84 ± 23.83 μmol/L after 24 hours (95% CI: −29.14 to −1.27; P = 0.03), with a moderate to large effect size (Cohen’s d = 0.59). Serum GAA levels showed a non-significant increase, from 3.27 ± 0.60 μmol/L to 3.47 ± 0.64 μmol/L (95% CI: −0.58 to 0.16; P = 0.26; Cohen’s d = 0.32). Serum creatinine levels remained unchanged, with no significant difference between baseline (100.57 ± 9.19 μmol/L) and follow-up (106.10 ± 27.90 μmol/L; 95% CI: −21.24 to 10.20; P = 0.46; Cohen’s d = 0.26).Conclusion Sleep deprivation alters creatine metabolism biomarkers, indicating shifts in metabolic pathways. Further research is necessary to validate and expand these findings, potentially elucidating the role of creatine in stress-related conditions.https://www.tandfonline.com/doi/10.1080/15502783.2025.2533643Inadequate sleepcreatinebiomarkersmetabolism
spellingShingle Nikola Todorovic
David Nedeljkovic
Jovana Panic
Sergej M. Ostojic
Impact of sleep deprivation on creatine metabolism biomarkers in healthy young males
Journal of the International Society of Sports Nutrition
Inadequate sleep
creatine
biomarkers
metabolism
title Impact of sleep deprivation on creatine metabolism biomarkers in healthy young males
title_full Impact of sleep deprivation on creatine metabolism biomarkers in healthy young males
title_fullStr Impact of sleep deprivation on creatine metabolism biomarkers in healthy young males
title_full_unstemmed Impact of sleep deprivation on creatine metabolism biomarkers in healthy young males
title_short Impact of sleep deprivation on creatine metabolism biomarkers in healthy young males
title_sort impact of sleep deprivation on creatine metabolism biomarkers in healthy young males
topic Inadequate sleep
creatine
biomarkers
metabolism
url https://www.tandfonline.com/doi/10.1080/15502783.2025.2533643
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AT davidnedeljkovic impactofsleepdeprivationoncreatinemetabolismbiomarkersinhealthyyoungmales
AT jovanapanic impactofsleepdeprivationoncreatinemetabolismbiomarkersinhealthyyoungmales
AT sergejmostojic impactofsleepdeprivationoncreatinemetabolismbiomarkersinhealthyyoungmales