Multifunctional MOF microneedle patch with adsorbed exosomes for enhanced diabetic wound healing
Chronic wounds present a significant clinical challenge for which advanced dressings with regenerative properties are essential for effective healing. This study developed an exosome (Exo)-loaded microneedle (MN) patch. The patch was fabricated based on a metal-organic framework (MOF) through a self...
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Elsevier
2025-08-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006425006465 |
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| author | Jieyu Xiang Ziming Li songlu Tseng Tianhao Li Liquan Wang Zhujun Li Lin Kang Fengzhou Du Jiuzuo Huang Nanze Yu Xiao Long |
| author_facet | Jieyu Xiang Ziming Li songlu Tseng Tianhao Li Liquan Wang Zhujun Li Lin Kang Fengzhou Du Jiuzuo Huang Nanze Yu Xiao Long |
| author_sort | Jieyu Xiang |
| collection | DOAJ |
| description | Chronic wounds present a significant clinical challenge for which advanced dressings with regenerative properties are essential for effective healing. This study developed an exosome (Exo)-loaded microneedle (MN) patch. The patch was fabricated based on a metal-organic framework (MOF) through a self-assembly electrostatic adsorption process, with the objective of enhancing wound healing. The large surface area, high porosity, and positive charge of the MOF enable the efficient loading of negatively charged exosomes via electrostatic interactions. As the MNs degrade, the MOFs release both zinc ions, with antibacterial, anti-inflammatory, and angiogenic properties, and exosomes, which are internalized by cells and enhance cellular regeneration. In vitro and in vivo studies confirmed the effectiveness of the MN-MOF-Exo patch in diabetic wound healing, while RNA sequencing analysis revealed that the patch accelerated wound healing by upregulating key genes, activating the ERK1/2 and PI3K–Akt signaling pathways, and facilitating angiogenesis, cell migration, and extracellular matrix remodeling. This innovative approach combines the efficient electrostatic self-adsorption of exosomes onto the MOF within a MN structure, enabling the precise, minimally invasive, and effective delivery of therapeutic agents directly to the wound site. Furthermore, the regenerative mechanisms of the MN-MOF-Exo patch were investigated, revealing how tissue repair is promoted and how healing is accelerated through enhanced cellular regeneration and localized therapeutic effects. |
| format | Article |
| id | doaj-art-68a0e3b04c384ad08d2c47e42c7c061e |
| institution | Matheson Library |
| issn | 2590-0064 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-68a0e3b04c384ad08d2c47e42c7c061e2025-07-17T04:44:49ZengElsevierMaterials Today Bio2590-00642025-08-0133102076Multifunctional MOF microneedle patch with adsorbed exosomes for enhanced diabetic wound healingJieyu Xiang0Ziming Li1songlu Tseng2Tianhao Li3Liquan Wang4Zhujun Li5Lin Kang6Fengzhou Du7Jiuzuo Huang8Nanze Yu9Xiao Long10Department of Plastic and Aesthetic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China; Center for Regenerative Medicine & Plastic Surgery Research, Peking Union Medical College Hospital, Beijing, ChinaDepartment of Plastic and Aesthetic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China; Center for Regenerative Medicine & Plastic Surgery Research, Peking Union Medical College Hospital, Beijing, ChinaDepartment of Plastic and Aesthetic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China; Center for Regenerative Medicine & Plastic Surgery Research, Peking Union Medical College Hospital, Beijing, ChinaDepartment of Plastic and Aesthetic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China; Center for Regenerative Medicine & Plastic Surgery Research, Peking Union Medical College Hospital, Beijing, ChinaDepartment of Plastic and Aesthetic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China; Center for Regenerative Medicine & Plastic Surgery Research, Peking Union Medical College Hospital, Beijing, ChinaDepartment of Plastic and Aesthetic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China; Center for Regenerative Medicine & Plastic Surgery Research, Peking Union Medical College Hospital, Beijing, ChinaBiomedical Engineering Facility, Institute of Clinical Medicine, Chinese Academy of Medical Sciences, Beijing, ChinaDepartment of Plastic and Aesthetic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China; Center for Regenerative Medicine & Plastic Surgery Research, Peking Union Medical College Hospital, Beijing, ChinaDepartment of Plastic and Aesthetic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China; Center for Regenerative Medicine & Plastic Surgery Research, Peking Union Medical College Hospital, Beijing, ChinaDepartment of Plastic and Aesthetic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China; Center for Regenerative Medicine & Plastic Surgery Research, Peking Union Medical College Hospital, Beijing, China; Corresponding author. Department of Plastic and Aesthetic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.Department of Plastic and Aesthetic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China; Center for Regenerative Medicine & Plastic Surgery Research, Peking Union Medical College Hospital, Beijing, China; Corresponding author. Department of Plastic and Aesthetic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.Chronic wounds present a significant clinical challenge for which advanced dressings with regenerative properties are essential for effective healing. This study developed an exosome (Exo)-loaded microneedle (MN) patch. The patch was fabricated based on a metal-organic framework (MOF) through a self-assembly electrostatic adsorption process, with the objective of enhancing wound healing. The large surface area, high porosity, and positive charge of the MOF enable the efficient loading of negatively charged exosomes via electrostatic interactions. As the MNs degrade, the MOFs release both zinc ions, with antibacterial, anti-inflammatory, and angiogenic properties, and exosomes, which are internalized by cells and enhance cellular regeneration. In vitro and in vivo studies confirmed the effectiveness of the MN-MOF-Exo patch in diabetic wound healing, while RNA sequencing analysis revealed that the patch accelerated wound healing by upregulating key genes, activating the ERK1/2 and PI3K–Akt signaling pathways, and facilitating angiogenesis, cell migration, and extracellular matrix remodeling. This innovative approach combines the efficient electrostatic self-adsorption of exosomes onto the MOF within a MN structure, enabling the precise, minimally invasive, and effective delivery of therapeutic agents directly to the wound site. Furthermore, the regenerative mechanisms of the MN-MOF-Exo patch were investigated, revealing how tissue repair is promoted and how healing is accelerated through enhanced cellular regeneration and localized therapeutic effects.http://www.sciencedirect.com/science/article/pii/S2590006425006465Diabetic wound healingExosomesMicroneedlesmetal‒organic frameworksElectrostatic adsorption |
| spellingShingle | Jieyu Xiang Ziming Li songlu Tseng Tianhao Li Liquan Wang Zhujun Li Lin Kang Fengzhou Du Jiuzuo Huang Nanze Yu Xiao Long Multifunctional MOF microneedle patch with adsorbed exosomes for enhanced diabetic wound healing Materials Today Bio Diabetic wound healing Exosomes Microneedles metal‒organic frameworks Electrostatic adsorption |
| title | Multifunctional MOF microneedle patch with adsorbed exosomes for enhanced diabetic wound healing |
| title_full | Multifunctional MOF microneedle patch with adsorbed exosomes for enhanced diabetic wound healing |
| title_fullStr | Multifunctional MOF microneedle patch with adsorbed exosomes for enhanced diabetic wound healing |
| title_full_unstemmed | Multifunctional MOF microneedle patch with adsorbed exosomes for enhanced diabetic wound healing |
| title_short | Multifunctional MOF microneedle patch with adsorbed exosomes for enhanced diabetic wound healing |
| title_sort | multifunctional mof microneedle patch with adsorbed exosomes for enhanced diabetic wound healing |
| topic | Diabetic wound healing Exosomes Microneedles metal‒organic frameworks Electrostatic adsorption |
| url | http://www.sciencedirect.com/science/article/pii/S2590006425006465 |
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