OsPPR9 encodes a DYW-type PPR protein that affects editing efficiency of multiple RNA editing sites and is essential for chloroplast development
Photosynthesis occurs mainly in chloroplasts, whose development is regulated by proteins encoded by nuclear genes. Among them, pentapeptide repeat (PPR) proteins participate in organelle RNA editing. Although there are more than 450 members of the PPR protein family in rice, only a few affect RNA ed...
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| Format: | Article |
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KeAi Communications Co., Ltd.
2023-04-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311922001046 |
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| author | Chang-zhao CHEN Ya-liang WANG Meng-xing HE Zhi-wen LI Lan SHEN Qing LI De-yong REN Jiang HU Li ZHU Guang-heng ZHANG Zhen-yu GAO Da-li ZENG Long-biao GUO Qian QIAN Qiang ZHANG |
| author_facet | Chang-zhao CHEN Ya-liang WANG Meng-xing HE Zhi-wen LI Lan SHEN Qing LI De-yong REN Jiang HU Li ZHU Guang-heng ZHANG Zhen-yu GAO Da-li ZENG Long-biao GUO Qian QIAN Qiang ZHANG |
| author_sort | Chang-zhao CHEN |
| collection | DOAJ |
| description | Photosynthesis occurs mainly in chloroplasts, whose development is regulated by proteins encoded by nuclear genes. Among them, pentapeptide repeat (PPR) proteins participate in organelle RNA editing. Although there are more than 450 members of the PPR protein family in rice, only a few affect RNA editing in rice chloroplasts. Gene editing technology has created new rice germplasm and mutants, which could be used for rice breeding and gene function study. This study evaluated the functions of OsPPR9 in chloroplast RNA editing in rice. The osppr9 mutants were obtained by CRISPR/Cas9, which showed yellowing leaves and a lethal phenotype, with suppressed expression of genes associated with chloroplast development and accumulation of photosynthetic-related proteins. In addition, loss of OsPPR9 protein function reduces the editing efficiency of rps8-C182, rpoC2-C4106, rps14-C80, and ndhB-C611 RNA editing sites, which affects chloroplast growth and development in rice. Our data showed that OsPPR9 is highly expressed in rice leaves and encodes a DYW-PPR protein localized in chloroplasts. Besides, the OsPPR9 protein was shown to interact with OsMORF2 and OsMORF9. Together, our findings provide insights into the role of the PPR protein in regulating chloroplast development in rice. |
| format | Article |
| id | doaj-art-673e2bc9be554f82b7a735a1cb025197 |
| institution | Matheson Library |
| issn | 2095-3119 |
| language | English |
| publishDate | 2023-04-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-673e2bc9be554f82b7a735a1cb0251972025-08-02T09:55:02ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192023-04-01224972980OsPPR9 encodes a DYW-type PPR protein that affects editing efficiency of multiple RNA editing sites and is essential for chloroplast developmentChang-zhao CHEN0Ya-liang WANG1Meng-xing HE2Zhi-wen LI3Lan SHEN4Qing LI5De-yong REN6Jiang HU7Li ZHU8Guang-heng ZHANG9Zhen-yu GAO10Da-li ZENG11Long-biao GUO12Qian QIAN13Qiang ZHANG14State Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou 310006, P.R.ChinaState Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou 310006, P.R.ChinaState Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou 310006, P.R.China; College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, P.R.ChinaState Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou 310006, P.R.China; College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310006, P.R.ChinaState Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou 310006, P.R.ChinaState Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou 310006, P.R.ChinaState Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou 310006, P.R.ChinaState Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou 310006, P.R.ChinaState Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou 310006, P.R.ChinaState Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou 310006, P.R.ChinaState Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou 310006, P.R.ChinaState Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou 310006, P.R.ChinaState Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou 310006, P.R.ChinaState Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou 310006, P.R.China; Correspondence QIAN qian, Tel: +86-571-63370378State Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou 310006, P.R.China; ZHANG Qiang, Tel: +86-571-63370378Photosynthesis occurs mainly in chloroplasts, whose development is regulated by proteins encoded by nuclear genes. Among them, pentapeptide repeat (PPR) proteins participate in organelle RNA editing. Although there are more than 450 members of the PPR protein family in rice, only a few affect RNA editing in rice chloroplasts. Gene editing technology has created new rice germplasm and mutants, which could be used for rice breeding and gene function study. This study evaluated the functions of OsPPR9 in chloroplast RNA editing in rice. The osppr9 mutants were obtained by CRISPR/Cas9, which showed yellowing leaves and a lethal phenotype, with suppressed expression of genes associated with chloroplast development and accumulation of photosynthetic-related proteins. In addition, loss of OsPPR9 protein function reduces the editing efficiency of rps8-C182, rpoC2-C4106, rps14-C80, and ndhB-C611 RNA editing sites, which affects chloroplast growth and development in rice. Our data showed that OsPPR9 is highly expressed in rice leaves and encodes a DYW-PPR protein localized in chloroplasts. Besides, the OsPPR9 protein was shown to interact with OsMORF2 and OsMORF9. Together, our findings provide insights into the role of the PPR protein in regulating chloroplast development in rice.http://www.sciencedirect.com/science/article/pii/S2095311922001046rice (Oryza sativa L.)PPR proteinchloroplast developmentRNA editing |
| spellingShingle | Chang-zhao CHEN Ya-liang WANG Meng-xing HE Zhi-wen LI Lan SHEN Qing LI De-yong REN Jiang HU Li ZHU Guang-heng ZHANG Zhen-yu GAO Da-li ZENG Long-biao GUO Qian QIAN Qiang ZHANG OsPPR9 encodes a DYW-type PPR protein that affects editing efficiency of multiple RNA editing sites and is essential for chloroplast development Journal of Integrative Agriculture rice (Oryza sativa L.) PPR protein chloroplast development RNA editing |
| title | OsPPR9 encodes a DYW-type PPR protein that affects editing efficiency of multiple RNA editing sites and is essential for chloroplast development |
| title_full | OsPPR9 encodes a DYW-type PPR protein that affects editing efficiency of multiple RNA editing sites and is essential for chloroplast development |
| title_fullStr | OsPPR9 encodes a DYW-type PPR protein that affects editing efficiency of multiple RNA editing sites and is essential for chloroplast development |
| title_full_unstemmed | OsPPR9 encodes a DYW-type PPR protein that affects editing efficiency of multiple RNA editing sites and is essential for chloroplast development |
| title_short | OsPPR9 encodes a DYW-type PPR protein that affects editing efficiency of multiple RNA editing sites and is essential for chloroplast development |
| title_sort | osppr9 encodes a dyw type ppr protein that affects editing efficiency of multiple rna editing sites and is essential for chloroplast development |
| topic | rice (Oryza sativa L.) PPR protein chloroplast development RNA editing |
| url | http://www.sciencedirect.com/science/article/pii/S2095311922001046 |
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