Morin inhibited lung cancer cells viability, growth, and migration by suppressing miR-135b and inducing its target CCNG2
Lung cancer is one of the most severe threats with the highest mortality rate to humans in the world. Recently, morin has been reported to have anti-tumor properties observed in several types of cancers. However, its mechanism is still unclear. We assessed the influences of morin on cell viability,...
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| Natura: | Articolo |
| Lingua: | inglese |
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SAGE Publishing
2017-09-01
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| Serie: | Tumor Biology |
| Accesso online: | https://doi.org/10.1177/1010428317712443 |
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| author | Dongjie Yao Hujun Cui Shufen Zhou Ling Guo |
| author_facet | Dongjie Yao Hujun Cui Shufen Zhou Ling Guo |
| author_sort | Dongjie Yao |
| collection | DOAJ |
| description | Lung cancer is one of the most severe threats with the highest mortality rate to humans in the world. Recently, morin has been reported to have anti-tumor properties observed in several types of cancers. However, its mechanism is still unclear. We assessed the influences of morin on cell viability, colony formation, and migration ability of A549 and employed microRNA array to identify the microRNAs affected by morin. We found that morin-treated A549 cells showed statistically decreased cell viability, colony formation, and migration rate when comparing with the dimethyl sulfoxide–treated cells. Microarray results showed that with the treatment of morin, the expression level of miR-135b significantly reduced compared the control group, suggesting that morin may exert its anti-cancer property by suppressing the expression of miR-135b. In addition, we found a potential binding site of miR-135b within 3′ untranslated region of CCNG2-encoding cyclin homolog cyclin-G2. We evidenced that miR-135b directly targets CCNG2, which could be a potential biomarker of lung cancer prognosis. Morin exerts its anti-tumor function via downregulating the expression of miR-135b that directly targets and represses CCNG2. |
| format | Article |
| id | doaj-art-64de4b1e6cc14ea884f2e68285dcedbb |
| institution | Matheson Library |
| issn | 1423-0380 |
| language | English |
| publishDate | 2017-09-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Tumor Biology |
| spelling | doaj-art-64de4b1e6cc14ea884f2e68285dcedbb2025-08-02T09:41:01ZengSAGE PublishingTumor Biology1423-03802017-09-013910.1177/1010428317712443Morin inhibited lung cancer cells viability, growth, and migration by suppressing miR-135b and inducing its target CCNG2Dongjie Yao0Hujun Cui1Shufen Zhou2Ling Guo3Department of Quality Control, Affiliated Second Hospital, Mudanjiang Medical University, Mudanjiang, ChinaDepartment of Oncology, Affiliated Hongqi Hospital, Mudanjiang Medical University, Mudanjiang, ChinaDepartment of Gerontology, Affiliated Second Hospital, Mudanjiang Medical University, Mudanjiang, ChinaDepartment of Pathology, Affiliated Second Hospital, Mudanjiang Medical University, Mudanjiang, ChinaLung cancer is one of the most severe threats with the highest mortality rate to humans in the world. Recently, morin has been reported to have anti-tumor properties observed in several types of cancers. However, its mechanism is still unclear. We assessed the influences of morin on cell viability, colony formation, and migration ability of A549 and employed microRNA array to identify the microRNAs affected by morin. We found that morin-treated A549 cells showed statistically decreased cell viability, colony formation, and migration rate when comparing with the dimethyl sulfoxide–treated cells. Microarray results showed that with the treatment of morin, the expression level of miR-135b significantly reduced compared the control group, suggesting that morin may exert its anti-cancer property by suppressing the expression of miR-135b. In addition, we found a potential binding site of miR-135b within 3′ untranslated region of CCNG2-encoding cyclin homolog cyclin-G2. We evidenced that miR-135b directly targets CCNG2, which could be a potential biomarker of lung cancer prognosis. Morin exerts its anti-tumor function via downregulating the expression of miR-135b that directly targets and represses CCNG2.https://doi.org/10.1177/1010428317712443 |
| spellingShingle | Dongjie Yao Hujun Cui Shufen Zhou Ling Guo Morin inhibited lung cancer cells viability, growth, and migration by suppressing miR-135b and inducing its target CCNG2 Tumor Biology |
| title | Morin inhibited lung cancer cells viability, growth, and migration by suppressing miR-135b and inducing its target CCNG2 |
| title_full | Morin inhibited lung cancer cells viability, growth, and migration by suppressing miR-135b and inducing its target CCNG2 |
| title_fullStr | Morin inhibited lung cancer cells viability, growth, and migration by suppressing miR-135b and inducing its target CCNG2 |
| title_full_unstemmed | Morin inhibited lung cancer cells viability, growth, and migration by suppressing miR-135b and inducing its target CCNG2 |
| title_short | Morin inhibited lung cancer cells viability, growth, and migration by suppressing miR-135b and inducing its target CCNG2 |
| title_sort | morin inhibited lung cancer cells viability growth and migration by suppressing mir 135b and inducing its target ccng2 |
| url | https://doi.org/10.1177/1010428317712443 |
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