GATA-3 EXPRESSION IN PERIPHERAL BLOOD LYMPHOCYTES OF PATIENTS WITH BRONCHIAL ASTHMA
The aim of the study is to establish the features of expression of GATA-3 in peripheral lymphocytes from bronchial asthma patients (BA). Material and methods. 10 healthy controls, 15 patients with allergic (atopic) and 15 persons with non-allergic BA were examined. A transcription factor GATA-3 expr...
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| Format: | Article |
| Language: | Russian |
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St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2014-07-01
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| Series: | Медицинская иммунология |
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| Online Access: | https://www.mimmun.ru/mimmun/article/view/35 |
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| author | V. N. Mineev L. N. Sorokina M. A. Nyoma V. I. Trofimov |
| author_facet | V. N. Mineev L. N. Sorokina M. A. Nyoma V. I. Trofimov |
| author_sort | V. N. Mineev |
| collection | DOAJ |
| description | The aim of the study is to establish the features of expression of GATA-3 in peripheral lymphocytes from bronchial asthma patients (BA). Material and methods. 10 healthy controls, 15 patients with allergic (atopic) and 15 persons with non-allergic BA were examined. A transcription factor GATA-3 expressed in peripheral lymphocytes was analyzed by Western blot after the lymphocytes were lysed. Preparation of cell lysates, and Western blotting were performed by means of a standard procedure (Amersham). An antibody against GATA-3 (Abcam, UK) was used. Levels of the protein were analyzed versus β-actin levels using anti-actin antibody (Sigma Aldrich, USA). Results. Expression of GATA-3 was significantly increased in lymphocytes of patients with allergic BA as compared to healthy persons and non-allergic BA patients. The level of GATA-3 negatively correlated with the degree of airflow obstruction and positively correlated with dosage of parenteral steroids administered. Conclusion. GATA-3 may play a key role in the pathophysiology of BA. One may suggest that increased expression of GATA-3 transcription factor in atopic BA underlie high levels of Th2-cytokines production in allergic disease |
| format | Article |
| id | doaj-art-642f7e243e204c67b9daa8c04ac8b806 |
| institution | Matheson Library |
| issn | 1563-0625 2313-741X |
| language | Russian |
| publishDate | 2014-07-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj-art-642f7e243e204c67b9daa8c04ac8b8062025-08-04T14:30:27ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2014-07-01121-2212810.15789/1563-0625-2010-1-2-21-2834GATA-3 EXPRESSION IN PERIPHERAL BLOOD LYMPHOCYTES OF PATIENTS WITH BRONCHIAL ASTHMAV. N. Mineev0L. N. Sorokina1M. A. Nyoma2V. I. Trofimov3Кафедра госпитальной терапии имени акад. М.В. Черноруцкого Санкт-Петербургского государственного медицинского университета имени акад. И.П. ПавловаКафедра госпитальной терапии имени акад. М.В. Черноруцкого Санкт-Петербургского государственного медицинского университета имени акад. И.П. ПавловаКафедра госпитальной терапии имени акад. М.В. Черноруцкого Санкт-Петербургского государственного медицинского университета имени акад. И.П. ПавловаКафедра госпитальной терапии имени акад. М.В. Черноруцкого Санкт-Петербургского государственного медицинского университета имени акад. И.П. ПавловаThe aim of the study is to establish the features of expression of GATA-3 in peripheral lymphocytes from bronchial asthma patients (BA). Material and methods. 10 healthy controls, 15 patients with allergic (atopic) and 15 persons with non-allergic BA were examined. A transcription factor GATA-3 expressed in peripheral lymphocytes was analyzed by Western blot after the lymphocytes were lysed. Preparation of cell lysates, and Western blotting were performed by means of a standard procedure (Amersham). An antibody against GATA-3 (Abcam, UK) was used. Levels of the protein were analyzed versus β-actin levels using anti-actin antibody (Sigma Aldrich, USA). Results. Expression of GATA-3 was significantly increased in lymphocytes of patients with allergic BA as compared to healthy persons and non-allergic BA patients. The level of GATA-3 negatively correlated with the degree of airflow obstruction and positively correlated with dosage of parenteral steroids administered. Conclusion. GATA-3 may play a key role in the pathophysiology of BA. One may suggest that increased expression of GATA-3 transcription factor in atopic BA underlie high levels of Th2-cytokines production in allergic diseasehttps://www.mimmun.ru/mimmun/article/view/35bronchial asthmath2-cytokinesgata-3lymphocytes |
| spellingShingle | V. N. Mineev L. N. Sorokina M. A. Nyoma V. I. Trofimov GATA-3 EXPRESSION IN PERIPHERAL BLOOD LYMPHOCYTES OF PATIENTS WITH BRONCHIAL ASTHMA Медицинская иммунология bronchial asthma th2-cytokines gata-3 lymphocytes |
| title | GATA-3 EXPRESSION IN PERIPHERAL BLOOD LYMPHOCYTES OF PATIENTS WITH BRONCHIAL ASTHMA |
| title_full | GATA-3 EXPRESSION IN PERIPHERAL BLOOD LYMPHOCYTES OF PATIENTS WITH BRONCHIAL ASTHMA |
| title_fullStr | GATA-3 EXPRESSION IN PERIPHERAL BLOOD LYMPHOCYTES OF PATIENTS WITH BRONCHIAL ASTHMA |
| title_full_unstemmed | GATA-3 EXPRESSION IN PERIPHERAL BLOOD LYMPHOCYTES OF PATIENTS WITH BRONCHIAL ASTHMA |
| title_short | GATA-3 EXPRESSION IN PERIPHERAL BLOOD LYMPHOCYTES OF PATIENTS WITH BRONCHIAL ASTHMA |
| title_sort | gata 3 expression in peripheral blood lymphocytes of patients with bronchial asthma |
| topic | bronchial asthma th2-cytokines gata-3 lymphocytes |
| url | https://www.mimmun.ru/mimmun/article/view/35 |
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