Simplified method for purifying full-length major type IV pilins: PilA from Acidithiobacillus thiooxidans
Type IV major pilins are membrane-associated proteins that play critical roles in bacterial adaptation and survival across diverse environmental conditions, including potentially extreme ones. However, their expression and purification remain challenging due to their hydrophobic nature and tendency...
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Main Authors: | Edgar D. Páez-Pérez, Araceli Hernández-Sánchez, Elvia Alfaro-Saldaña, J. Viridiana García-Meza |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2025-12-01
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Series: | MethodsX |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016125003644 |
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