Hydrothermally Synthesized TiO<sub>2</sub> Nanostructures for Electrochemical Detection of H<sub>2</sub>O<sub>2</sub> in Barley (<i>Hordeum vulgare</i>) Under Salt Stress and Remediation with Fe<sub>3</sub>O<sub>4</sub> Nanoparticles
This study presents the development of a TiO<sub>2</sub> nanowire-based electrochemical sensor for the selective and sensitive detection of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) under neutral pH conditions, with a particular focus on its application in an...
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2025-07-01
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| Series: | Chemosensors |
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| Online Access: | https://www.mdpi.com/2227-9040/13/7/256 |
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| author | Irena Mihailova Marina Krasovska Eriks Sledevskis Vjaceslavs Gerbreders Jans Keviss Valdis Mizers Inese Kokina Ilona Plaksenkova Marija Jermalonoka Aleksandra Mosenoka |
| author_facet | Irena Mihailova Marina Krasovska Eriks Sledevskis Vjaceslavs Gerbreders Jans Keviss Valdis Mizers Inese Kokina Ilona Plaksenkova Marija Jermalonoka Aleksandra Mosenoka |
| author_sort | Irena Mihailova |
| collection | DOAJ |
| description | This study presents the development of a TiO<sub>2</sub> nanowire-based electrochemical sensor for the selective and sensitive detection of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) under neutral pH conditions, with a particular focus on its application in analyzing plant stress. The sensor exhibited a linear detection range of 0–0.5 mM, a sensitivity of 0.0393 mA · mM<sup>−1</sup>, and a detection limit of 2.8 μM in phosphate-buffered saline solution (PBS, pH 7.4). This work’s main novelty lies in the systematic investigation of the relationship between TiO<sub>2</sub> nanostructure morphology, which is controlled by hydrothermal synthesis parameters, and the resulting sensor performance. Interference studies confirmed excellent selectivity in the presence of common electroactive species found in plant samples, such as NaCl, KNO<sub>3</sub>, glucose, citric acid, and ascorbic acid. Real sample analysis using barley plant extracts grown under salt stress and treated with Fe<sub>3</sub>O<sub>4</sub> nanoparticles confirmed the sensor’s applicability in complex biological matrices, enabling accurate quantification of endogenously produced H<sub>2</sub>O<sub>2</sub>. Endogenous H<sub>2</sub>O<sub>2</sub> concentrations were found to range from near-zero levels in control and Fe<sub>3</sub>O<sub>4</sub>-only treated plants, to elevated levels of up to 0.36 mM in salt-stressed samples. These levels decreased to 0.25 and 0.15 mM upon Fe<sub>3</sub>O<sub>4</sub> nanoparticle treatment, indicating a dose-dependent mitigation of stress. This finding was supported by genome template stability (GTS) analysis, which revealed improved DNA integrity in Fe<sub>3</sub>O<sub>4</sub>-treated plants. This study takes an integrated approach, combining the development of a nanostructured sensor with physiological and molecular stress assessment. The urgent need for tools to detect stress at an early stage and manage oxidative stress in sustainable agriculture underscores its relevance. |
| format | Article |
| id | doaj-art-62a3547f543a4f9f9030168d24c6a892 |
| institution | Matheson Library |
| issn | 2227-9040 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
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| series | Chemosensors |
| spelling | doaj-art-62a3547f543a4f9f9030168d24c6a8922025-07-25T13:18:05ZengMDPI AGChemosensors2227-90402025-07-0113725610.3390/chemosensors13070256Hydrothermally Synthesized TiO<sub>2</sub> Nanostructures for Electrochemical Detection of H<sub>2</sub>O<sub>2</sub> in Barley (<i>Hordeum vulgare</i>) Under Salt Stress and Remediation with Fe<sub>3</sub>O<sub>4</sub> NanoparticlesIrena Mihailova0Marina Krasovska1Eriks Sledevskis2Vjaceslavs Gerbreders3Jans Keviss4Valdis Mizers5Inese Kokina6Ilona Plaksenkova7Marija Jermalonoka8Aleksandra Mosenoka9G. Liberts’ Innovative Microscopy Centre, Department of Technology, Institute of Life Sciences and Technology, Daugavpils University, 1a Parades Str., LV-5401 Daugavpils, LatviaG. Liberts’ Innovative Microscopy Centre, Department of Technology, Institute of Life Sciences and Technology, Daugavpils University, 1a Parades Str., LV-5401 Daugavpils, LatviaG. Liberts’ Innovative Microscopy Centre, Department of Technology, Institute of Life Sciences and Technology, Daugavpils University, 1a Parades Str., LV-5401 Daugavpils, LatviaG. Liberts’ Innovative Microscopy Centre, Department of Technology, Institute of Life Sciences and Technology, Daugavpils University, 1a Parades Str., LV-5401 Daugavpils, LatviaG. Liberts’ Innovative Microscopy Centre, Department of Technology, Institute of Life Sciences and Technology, Daugavpils University, 1a Parades Str., LV-5401 Daugavpils, LatviaG. Liberts’ Innovative Microscopy Centre, Department of Technology, Institute of Life Sciences and Technology, Daugavpils University, 1a Parades Str., LV-5401 Daugavpils, LatviaLaboratory of Genomic and Biotechnology, Department of Technology, Institute of Life Sciences and Technology, Daugavpils University, 1a Parades Str., LV-5401 Daugavpils, LatviaLaboratory of Genomic and Biotechnology, Department of Technology, Institute of Life Sciences and Technology, Daugavpils University, 1a Parades Str., LV-5401 Daugavpils, LatviaLaboratory of Genomic and Biotechnology, Department of Technology, Institute of Life Sciences and Technology, Daugavpils University, 1a Parades Str., LV-5401 Daugavpils, LatviaLaboratory of Genomic and Biotechnology, Department of Technology, Institute of Life Sciences and Technology, Daugavpils University, 1a Parades Str., LV-5401 Daugavpils, LatviaThis study presents the development of a TiO<sub>2</sub> nanowire-based electrochemical sensor for the selective and sensitive detection of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) under neutral pH conditions, with a particular focus on its application in analyzing plant stress. The sensor exhibited a linear detection range of 0–0.5 mM, a sensitivity of 0.0393 mA · mM<sup>−1</sup>, and a detection limit of 2.8 μM in phosphate-buffered saline solution (PBS, pH 7.4). This work’s main novelty lies in the systematic investigation of the relationship between TiO<sub>2</sub> nanostructure morphology, which is controlled by hydrothermal synthesis parameters, and the resulting sensor performance. Interference studies confirmed excellent selectivity in the presence of common electroactive species found in plant samples, such as NaCl, KNO<sub>3</sub>, glucose, citric acid, and ascorbic acid. Real sample analysis using barley plant extracts grown under salt stress and treated with Fe<sub>3</sub>O<sub>4</sub> nanoparticles confirmed the sensor’s applicability in complex biological matrices, enabling accurate quantification of endogenously produced H<sub>2</sub>O<sub>2</sub>. Endogenous H<sub>2</sub>O<sub>2</sub> concentrations were found to range from near-zero levels in control and Fe<sub>3</sub>O<sub>4</sub>-only treated plants, to elevated levels of up to 0.36 mM in salt-stressed samples. These levels decreased to 0.25 and 0.15 mM upon Fe<sub>3</sub>O<sub>4</sub> nanoparticle treatment, indicating a dose-dependent mitigation of stress. This finding was supported by genome template stability (GTS) analysis, which revealed improved DNA integrity in Fe<sub>3</sub>O<sub>4</sub>-treated plants. This study takes an integrated approach, combining the development of a nanostructured sensor with physiological and molecular stress assessment. The urgent need for tools to detect stress at an early stage and manage oxidative stress in sustainable agriculture underscores its relevance.https://www.mdpi.com/2227-9040/13/7/256electrochemical sensorTiO<sub>2</sub> nanostructuresH<sub>2</sub>O<sub>2</sub> detectionhydrothermal synthesisplant stress |
| spellingShingle | Irena Mihailova Marina Krasovska Eriks Sledevskis Vjaceslavs Gerbreders Jans Keviss Valdis Mizers Inese Kokina Ilona Plaksenkova Marija Jermalonoka Aleksandra Mosenoka Hydrothermally Synthesized TiO<sub>2</sub> Nanostructures for Electrochemical Detection of H<sub>2</sub>O<sub>2</sub> in Barley (<i>Hordeum vulgare</i>) Under Salt Stress and Remediation with Fe<sub>3</sub>O<sub>4</sub> Nanoparticles Chemosensors electrochemical sensor TiO<sub>2</sub> nanostructures H<sub>2</sub>O<sub>2</sub> detection hydrothermal synthesis plant stress |
| title | Hydrothermally Synthesized TiO<sub>2</sub> Nanostructures for Electrochemical Detection of H<sub>2</sub>O<sub>2</sub> in Barley (<i>Hordeum vulgare</i>) Under Salt Stress and Remediation with Fe<sub>3</sub>O<sub>4</sub> Nanoparticles |
| title_full | Hydrothermally Synthesized TiO<sub>2</sub> Nanostructures for Electrochemical Detection of H<sub>2</sub>O<sub>2</sub> in Barley (<i>Hordeum vulgare</i>) Under Salt Stress and Remediation with Fe<sub>3</sub>O<sub>4</sub> Nanoparticles |
| title_fullStr | Hydrothermally Synthesized TiO<sub>2</sub> Nanostructures for Electrochemical Detection of H<sub>2</sub>O<sub>2</sub> in Barley (<i>Hordeum vulgare</i>) Under Salt Stress and Remediation with Fe<sub>3</sub>O<sub>4</sub> Nanoparticles |
| title_full_unstemmed | Hydrothermally Synthesized TiO<sub>2</sub> Nanostructures for Electrochemical Detection of H<sub>2</sub>O<sub>2</sub> in Barley (<i>Hordeum vulgare</i>) Under Salt Stress and Remediation with Fe<sub>3</sub>O<sub>4</sub> Nanoparticles |
| title_short | Hydrothermally Synthesized TiO<sub>2</sub> Nanostructures for Electrochemical Detection of H<sub>2</sub>O<sub>2</sub> in Barley (<i>Hordeum vulgare</i>) Under Salt Stress and Remediation with Fe<sub>3</sub>O<sub>4</sub> Nanoparticles |
| title_sort | hydrothermally synthesized tio sub 2 sub nanostructures for electrochemical detection of h sub 2 sub o sub 2 sub in barley i hordeum vulgare i under salt stress and remediation with fe sub 3 sub o sub 4 sub nanoparticles |
| topic | electrochemical sensor TiO<sub>2</sub> nanostructures H<sub>2</sub>O<sub>2</sub> detection hydrothermal synthesis plant stress |
| url | https://www.mdpi.com/2227-9040/13/7/256 |
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