Features of the dynamics of serum C-reactive protein and IL-6 concentrations in case of experimental carrageenan-induced inflammation of temporomandibular joints
Background. The temporomandibular joint is a complex anatomical and functional structure, consisting of the mandibular condyle, mandibular fossa, articular tubercle of the temporal bone, intra-articular disc, and joint capsule. Temporomandibular joint movements are carried out through the highly coo...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
V. N. Karazin Kharkiv National University
2025-04-01
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| Series: | Journal of V. N. Karazin Kharkiv National University: Series Medicine |
| Subjects: | |
| Online Access: | https://ukrmedsci.com/index.php/visnyk/article/view/181 |
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| Summary: | Background. The temporomandibular joint is a complex anatomical and functional structure, consisting of the mandibular condyle, mandibular fossa, articular tubercle of the temporal bone, intra-articular disc, and joint capsule. Temporomandibular joint movements are carried out through the highly coordinated activity of all its components, primarily the masseter muscles, which are controlled by the nervous system.
Purpose – is to determine the dynamics of serum C-reactive protein and IL-6 concentrations in case of experimental carrageenan-induced inflammation of temporomandibular joints.
Materials and Methods. The experimental study was conducted on 36 mature male rats receiving injections of 0.02 mL of 1% carrageenan (Sigma-Aldrich, St. Louis, Missouri, USA) in saline (carrageenan group) and 0.02 mL of 0.9% saline (control) into both temporomandibular joints. The serum C-reactive protein and IL-6 concentrations was determined by immunoenzyme method using the Stat Fax 2100 Microplate Reader (Awareness Technology, Ins.) and the corresponding Rat ELISA Kits (MyBioSource, USA).
Results and discussion. The serum C-reactive protein and IL-6 concentration was intermittent throughout the entire period of experimental carrageenan-induced inflammation of temporomandibular joints. The C-reactive protein and IL-6 dynamic pattern shows the activation and subsequent attenuation of the inflammatory response and emphasizes the importance of simultaneous analysis of both markers for a more accurate assessment of the phase and severity of inflammation. The study results indicate that peak IL-6 and CRP concentrations were observed on the first days of induced inflammation. The increase in CRP (Day 1) and IL-6 levels (Day 3) shows phasic inflammation. A subsequent gradual decrease in CRP and IL-6 concentrations by Day 15 reflects the attenuation of inflammation and the activation of regeneration mechanisms. This indicates the reduction of acute inflammation and possible regeneration in the TMJ tissues.
Conclusion. Experimental carrageenan-induced inflammation of temporomandibular joints changes serum C-reactive protein and IL-6 levels showing the activation of the systemic inflammatory response. The C-reactive protein and IL-6 dynamic concentrations can be used to assess the course of temporomandibular joint inflammation. |
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| ISSN: | 2313-6693 2313-2396 |