Tannic acid-iron stabilized probiotic silver nano hybrids: Multi-target gut microbiota modulation and intestinal barrier restoration
The limited efficacy and adverse effect profile of current pharmacological treatments for intestinal inflammation underscore the need for modalities that preserve gut microbiota balance while attenuating inflammation. The aim of this study was to develop and evaluate a BL@TA-FeIII@AgNPs system with...
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Elsevier
2025-08-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006425006763 |
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| author | Saisai Gong Zhibo Zeng Mingjue Liu Xianfu Wang Chuxian Quan Muhammed Farhan Rahim Yaping Wang Aoyun Li Md. F. Kulyar Zhexue Lu Jiakui Li |
| author_facet | Saisai Gong Zhibo Zeng Mingjue Liu Xianfu Wang Chuxian Quan Muhammed Farhan Rahim Yaping Wang Aoyun Li Md. F. Kulyar Zhexue Lu Jiakui Li |
| author_sort | Saisai Gong |
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| description | The limited efficacy and adverse effect profile of current pharmacological treatments for intestinal inflammation underscore the need for modalities that preserve gut microbiota balance while attenuating inflammation. The aim of this study was to develop and evaluate a BL@TA-FeIII@AgNPs system with a view to provide synergistic efficacy against intestinal injury. This research introduces an innovative hybrid bio nanocomposite, BL@TA-FeIII@AgNPs, comprising viable Bacillus licheniformis coated with a tannic-acid/FeIII coordination layer that nucleates and anchors 7 ± 1.5 nm silver nanoparticles. Characterization of this composite material was performed using TEM, EDS, XRD, and XPS. Functional assays included probiotic viability, tolerance to simulated gastric and intestinal fluids, and bactericidal activity against Escherichia coli and Staphylococcus aureus. In-depth safety evaluations were carried out using both cell cultures and a mouse model. Therapeutic effects in an acute LPS-endotoxemia mouse model were analyzed by 16S rRNA gene amplicon sequencing and untargeted LC-MS/MS metabolomics of cecal contents. Characterization confirmed structural integrity, colloidal stability in physiological media, and low cytotoxicity (IC50 > 100 μg Ag mL−1). BL@TA-FeIII@AgNPs restored transepithelial electrical resistance, lowered malondialdehyde levels, and reshaped microbiota composition and metabolite networks relative to LPS controls. Restoration of the Firmicutes: Bacteroidetes ratio and elevated short chain fatty acid concentrations support BL@TA-FeIII@AgNPs as a promising adjunctive strategy for acute endotoxin-induced intestinal injury. |
| format | Article |
| id | doaj-art-609bb99a94d74cb0b2e1f5e6a86ed7bd |
| institution | Matheson Library |
| issn | 2590-0064 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
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| series | Materials Today Bio |
| spelling | doaj-art-609bb99a94d74cb0b2e1f5e6a86ed7bd2025-07-21T04:10:00ZengElsevierMaterials Today Bio2590-00642025-08-0133102106Tannic acid-iron stabilized probiotic silver nano hybrids: Multi-target gut microbiota modulation and intestinal barrier restorationSaisai Gong0Zhibo Zeng1Mingjue Liu2Xianfu Wang3Chuxian Quan4Muhammed Farhan Rahim5Yaping Wang6Aoyun Li7Md. F. Kulyar8Zhexue Lu9Jiakui Li10College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaCollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaCollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaCollege of Chemistry, Huazhong Agricultural University, Wuhan, ChinaCollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaCollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou, ChinaCollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China; Corresponding author.College of Chemistry, Huazhong Agricultural University, Wuhan, China; Corresponding author.College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China; College of Animals Husbandry and Veterinary Medicine, Tibet Agriculture and Animal Husbandry University, Linzhi, China; Corresponding author. College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.The limited efficacy and adverse effect profile of current pharmacological treatments for intestinal inflammation underscore the need for modalities that preserve gut microbiota balance while attenuating inflammation. The aim of this study was to develop and evaluate a BL@TA-FeIII@AgNPs system with a view to provide synergistic efficacy against intestinal injury. This research introduces an innovative hybrid bio nanocomposite, BL@TA-FeIII@AgNPs, comprising viable Bacillus licheniformis coated with a tannic-acid/FeIII coordination layer that nucleates and anchors 7 ± 1.5 nm silver nanoparticles. Characterization of this composite material was performed using TEM, EDS, XRD, and XPS. Functional assays included probiotic viability, tolerance to simulated gastric and intestinal fluids, and bactericidal activity against Escherichia coli and Staphylococcus aureus. In-depth safety evaluations were carried out using both cell cultures and a mouse model. Therapeutic effects in an acute LPS-endotoxemia mouse model were analyzed by 16S rRNA gene amplicon sequencing and untargeted LC-MS/MS metabolomics of cecal contents. Characterization confirmed structural integrity, colloidal stability in physiological media, and low cytotoxicity (IC50 > 100 μg Ag mL−1). BL@TA-FeIII@AgNPs restored transepithelial electrical resistance, lowered malondialdehyde levels, and reshaped microbiota composition and metabolite networks relative to LPS controls. Restoration of the Firmicutes: Bacteroidetes ratio and elevated short chain fatty acid concentrations support BL@TA-FeIII@AgNPs as a promising adjunctive strategy for acute endotoxin-induced intestinal injury.http://www.sciencedirect.com/science/article/pii/S2590006425006763ProbioticsSilver nanoparticlesGut microbiotaIntestinal inflammationNanomedicineBio-nanocomposites |
| spellingShingle | Saisai Gong Zhibo Zeng Mingjue Liu Xianfu Wang Chuxian Quan Muhammed Farhan Rahim Yaping Wang Aoyun Li Md. F. Kulyar Zhexue Lu Jiakui Li Tannic acid-iron stabilized probiotic silver nano hybrids: Multi-target gut microbiota modulation and intestinal barrier restoration Materials Today Bio Probiotics Silver nanoparticles Gut microbiota Intestinal inflammation Nanomedicine Bio-nanocomposites |
| title | Tannic acid-iron stabilized probiotic silver nano hybrids: Multi-target gut microbiota modulation and intestinal barrier restoration |
| title_full | Tannic acid-iron stabilized probiotic silver nano hybrids: Multi-target gut microbiota modulation and intestinal barrier restoration |
| title_fullStr | Tannic acid-iron stabilized probiotic silver nano hybrids: Multi-target gut microbiota modulation and intestinal barrier restoration |
| title_full_unstemmed | Tannic acid-iron stabilized probiotic silver nano hybrids: Multi-target gut microbiota modulation and intestinal barrier restoration |
| title_short | Tannic acid-iron stabilized probiotic silver nano hybrids: Multi-target gut microbiota modulation and intestinal barrier restoration |
| title_sort | tannic acid iron stabilized probiotic silver nano hybrids multi target gut microbiota modulation and intestinal barrier restoration |
| topic | Probiotics Silver nanoparticles Gut microbiota Intestinal inflammation Nanomedicine Bio-nanocomposites |
| url | http://www.sciencedirect.com/science/article/pii/S2590006425006763 |
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