Features of proteomic profi le of blood serum of children with bronchial asthma who underwent perinatal central nervous system damage
Objective: proteomic analysis of blood serum of children with bronchial asthma with a history of indication of the pathology of the perinatal period.Materials and methods: a comprehensive examination of children suffering from asthma and patients of the control group using proteomic analysis of bloo...
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| Format: | Article |
| Language: | English |
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State Budget Educational Institute of Higher Professional Education, Rostov State Medical University, Ministry Health of Russian Federation
2019-12-01
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| Series: | Медицинский вестник Юга России |
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| Online Access: | https://www.medicalherald.ru/jour/article/view/939 |
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| author | A. A. Lebedenko A. A. Afonin I. G. Loginova O. E. Semernik V. O. Gunko I. A. Alliluev A. V. Larichkin |
| author_facet | A. A. Lebedenko A. A. Afonin I. G. Loginova O. E. Semernik V. O. Gunko I. A. Alliluev A. V. Larichkin |
| author_sort | A. A. Lebedenko |
| collection | DOAJ |
| description | Objective: proteomic analysis of blood serum of children with bronchial asthma with a history of indication of the pathology of the perinatal period.Materials and methods: a comprehensive examination of children suffering from asthma and patients of the control group using proteomic analysis of blood serum was carried out. Proteomic analysis of blood serum was performed using two-dimensional high-resolution gel electrophoresis followed by staining of gels with fl uorescent dye and time-of-fl ight MALDI mass spectrometry. The proteins were identifi ed using Mascot MS peptide fi ngerprint analysis algorithm and Swiss-Prot protein database.Results: comparative analysis of the proteomic profi le of blood serum of children with pathology of the central nervous system in the perinatal period and developed in the subsequent bronchial asthma and control group patients allowed to establish that number proteins products were reduced in this disease. The proteins in the molecular mass range 16-33 kDa (p<0.05) were identifi ed: glutathione peroxidase 3, transthyretin and system components compliment С4В and C3.Conclusion: modifi cation of the production of these proteins indicates the presence of damage at the molecular level, leading to metabolic and functional disorders in the body of a patient with asthma having a history of indicating the pathology of the perinatal period. |
| format | Article |
| id | doaj-art-5ef9ed2ea91b45caa1a7267e2d92d123 |
| institution | Matheson Library |
| issn | 2219-8075 2618-7876 |
| language | English |
| publishDate | 2019-12-01 |
| publisher | State Budget Educational Institute of Higher Professional Education, Rostov State Medical University, Ministry Health of Russian Federation |
| record_format | Article |
| series | Медицинский вестник Юга России |
| spelling | doaj-art-5ef9ed2ea91b45caa1a7267e2d92d1232025-07-03T16:13:39ZengState Budget Educational Institute of Higher Professional Education, Rostov State Medical University, Ministry Health of Russian FederationМедицинский вестник Юга России2219-80752618-78762019-12-01104596510.21886/2219-8075-2019-10-4-59-65524Features of proteomic profi le of blood serum of children with bronchial asthma who underwent perinatal central nervous system damageA. A. Lebedenko0A. A. Afonin1I. G. Loginova2O. E. Semernik3V. O. Gunko4I. A. Alliluev5A. V. Larichkin6Rostov State Medical UniversityRostov State Medical UniversityRostov State Medical UniversityRostov State Medical UniversityRostov State Medical UniversityRostov State Medical UniversityRostov State Medical UniversityObjective: proteomic analysis of blood serum of children with bronchial asthma with a history of indication of the pathology of the perinatal period.Materials and methods: a comprehensive examination of children suffering from asthma and patients of the control group using proteomic analysis of blood serum was carried out. Proteomic analysis of blood serum was performed using two-dimensional high-resolution gel electrophoresis followed by staining of gels with fl uorescent dye and time-of-fl ight MALDI mass spectrometry. The proteins were identifi ed using Mascot MS peptide fi ngerprint analysis algorithm and Swiss-Prot protein database.Results: comparative analysis of the proteomic profi le of blood serum of children with pathology of the central nervous system in the perinatal period and developed in the subsequent bronchial asthma and control group patients allowed to establish that number proteins products were reduced in this disease. The proteins in the molecular mass range 16-33 kDa (p<0.05) were identifi ed: glutathione peroxidase 3, transthyretin and system components compliment С4В and C3.Conclusion: modifi cation of the production of these proteins indicates the presence of damage at the molecular level, leading to metabolic and functional disorders in the body of a patient with asthma having a history of indicating the pathology of the perinatal period.https://www.medicalherald.ru/jour/article/view/939bronchial asthmaproteomic analysis of perinatal lesion of central nervous systemchildrendiagnostics |
| spellingShingle | A. A. Lebedenko A. A. Afonin I. G. Loginova O. E. Semernik V. O. Gunko I. A. Alliluev A. V. Larichkin Features of proteomic profi le of blood serum of children with bronchial asthma who underwent perinatal central nervous system damage Медицинский вестник Юга России bronchial asthma proteomic analysis of perinatal lesion of central nervous system children diagnostics |
| title | Features of proteomic profi le of blood serum of children with bronchial asthma who underwent perinatal central nervous system damage |
| title_full | Features of proteomic profi le of blood serum of children with bronchial asthma who underwent perinatal central nervous system damage |
| title_fullStr | Features of proteomic profi le of blood serum of children with bronchial asthma who underwent perinatal central nervous system damage |
| title_full_unstemmed | Features of proteomic profi le of blood serum of children with bronchial asthma who underwent perinatal central nervous system damage |
| title_short | Features of proteomic profi le of blood serum of children with bronchial asthma who underwent perinatal central nervous system damage |
| title_sort | features of proteomic profi le of blood serum of children with bronchial asthma who underwent perinatal central nervous system damage |
| topic | bronchial asthma proteomic analysis of perinatal lesion of central nervous system children diagnostics |
| url | https://www.medicalherald.ru/jour/article/view/939 |
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