Features of immunoregulation in patients with pulmonary tuberculosis with blood eosinophilia
The aim of the investigation was to determine the characteristics of the immune response regulation for pulmonary tuberculosis (TB) and to analyze the role of regulatory T cells in the immunopathogenesis of TB with eosinophilia in the blood, depending on the clinical form of the disease and sensitiv...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Siberian State Medical University (Tomsk)
2018-09-01
|
| Series: | Бюллетень сибирской медицины |
| Subjects: | |
| Online Access: | https://bulletin.ssmu.ru/jour/article/view/1296 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1839569983380652032 |
|---|---|
| author | O. I. Urazova E. G. Churina Yu. V. Kolobovnikova V. V. Novitskiy A. V. Karaulov E. L. Nikulina V. S. Poletika |
| author_facet | O. I. Urazova E. G. Churina Yu. V. Kolobovnikova V. V. Novitskiy A. V. Karaulov E. L. Nikulina V. S. Poletika |
| author_sort | O. I. Urazova |
| collection | DOAJ |
| description | The aim of the investigation was to determine the characteristics of the immune response regulation for pulmonary tuberculosis (TB) and to analyze the role of regulatory T cells in the immunopathogenesis of TB with eosinophilia in the blood, depending on the clinical form of the disease and sensitivity of Micobacterium tuberculosis to anti-TB drugs.Materials and methods. 157 patients who were initially diagnosed with infiltrative and disseminated TB were examined. The material of the study was venous blood and culture of mononuclear leukocytes isolated from venous blood. The content of interleukin (IL) 4, IL-10 and transforming factor beta (TGFβ) in culture suspensions of mononuclear leukocytes in vitro and IL-5 in the blood was determined by enzyme-linked immunosorbent assay (ELISA) test. The expression of surface molecules CD4, CD20, CD25 and intracellular transcription factor Foxp3 by lymphocytes of the blood was evaluated by flow cytometry. The obtained results were analyzed by statistical methods.Results. It is shown that excessive generation of regulatory T cells in patients with TB is associated with eosinophilia of the blood and imbalance of immune response regulation mechanisms. In TB with eosinophilia, an increase in the number of Foxp3-positive regulatory T cells in the blood is combined with in vitro hypersecretion of anti-inflammatory cytokines TGFβ, IL-10, IL-4 and an increase in the content of CD20+ B lymphocytes and IL-5 in the blood. These changes are most pronounced in the disseminated form of TB in combination with drug resistance.Conclusion. Characteristics of immunoregulation at TB with blood eosinophilia are associated with activation of immunosuppression mechanisms and polarization of immune response towards Th2-dependent pathway. |
| format | Article |
| id | doaj-art-5ec2b709334841569dc32db6ac3036c6 |
| institution | Matheson Library |
| issn | 1682-0363 1819-3684 |
| language | English |
| publishDate | 2018-09-01 |
| publisher | Siberian State Medical University (Tomsk) |
| record_format | Article |
| series | Бюллетень сибирской медицины |
| spelling | doaj-art-5ec2b709334841569dc32db6ac3036c62025-08-04T16:45:41ZengSiberian State Medical University (Tomsk)Бюллетень сибирской медицины1682-03631819-36842018-09-0117316817910.20538/1682-0363-2018-3-168-179820Features of immunoregulation in patients with pulmonary tuberculosis with blood eosinophiliaO. I. Urazova0E. G. Churina1Yu. V. Kolobovnikova2V. V. Novitskiy3A. V. Karaulov4E. L. Nikulina5V. S. Poletika6Siberian State Medical University (SSMU)Siberian State Medical University (SSMU); National Research Tomsk State University (NR TSU)Siberian State Medical University (SSMU)Siberian State Medical University (SSMU)I.M. Sechenov First Moscow State Medical University Sechenov UniversitySiberian State Medical University (SSMU)Siberian State Medical University (SSMU)The aim of the investigation was to determine the characteristics of the immune response regulation for pulmonary tuberculosis (TB) and to analyze the role of regulatory T cells in the immunopathogenesis of TB with eosinophilia in the blood, depending on the clinical form of the disease and sensitivity of Micobacterium tuberculosis to anti-TB drugs.Materials and methods. 157 patients who were initially diagnosed with infiltrative and disseminated TB were examined. The material of the study was venous blood and culture of mononuclear leukocytes isolated from venous blood. The content of interleukin (IL) 4, IL-10 and transforming factor beta (TGFβ) in culture suspensions of mononuclear leukocytes in vitro and IL-5 in the blood was determined by enzyme-linked immunosorbent assay (ELISA) test. The expression of surface molecules CD4, CD20, CD25 and intracellular transcription factor Foxp3 by lymphocytes of the blood was evaluated by flow cytometry. The obtained results were analyzed by statistical methods.Results. It is shown that excessive generation of regulatory T cells in patients with TB is associated with eosinophilia of the blood and imbalance of immune response regulation mechanisms. In TB with eosinophilia, an increase in the number of Foxp3-positive regulatory T cells in the blood is combined with in vitro hypersecretion of anti-inflammatory cytokines TGFβ, IL-10, IL-4 and an increase in the content of CD20+ B lymphocytes and IL-5 in the blood. These changes are most pronounced in the disseminated form of TB in combination with drug resistance.Conclusion. Characteristics of immunoregulation at TB with blood eosinophilia are associated with activation of immunosuppression mechanisms and polarization of immune response towards Th2-dependent pathway.https://bulletin.ssmu.ru/jour/article/view/1296pulmonary tuberculosisimmune response regulationregulatory t cellseosinophilia |
| spellingShingle | O. I. Urazova E. G. Churina Yu. V. Kolobovnikova V. V. Novitskiy A. V. Karaulov E. L. Nikulina V. S. Poletika Features of immunoregulation in patients with pulmonary tuberculosis with blood eosinophilia Бюллетень сибирской медицины pulmonary tuberculosis immune response regulation regulatory t cells eosinophilia |
| title | Features of immunoregulation in patients with pulmonary tuberculosis with blood eosinophilia |
| title_full | Features of immunoregulation in patients with pulmonary tuberculosis with blood eosinophilia |
| title_fullStr | Features of immunoregulation in patients with pulmonary tuberculosis with blood eosinophilia |
| title_full_unstemmed | Features of immunoregulation in patients with pulmonary tuberculosis with blood eosinophilia |
| title_short | Features of immunoregulation in patients with pulmonary tuberculosis with blood eosinophilia |
| title_sort | features of immunoregulation in patients with pulmonary tuberculosis with blood eosinophilia |
| topic | pulmonary tuberculosis immune response regulation regulatory t cells eosinophilia |
| url | https://bulletin.ssmu.ru/jour/article/view/1296 |
| work_keys_str_mv | AT oiurazova featuresofimmunoregulationinpatientswithpulmonarytuberculosiswithbloodeosinophilia AT egchurina featuresofimmunoregulationinpatientswithpulmonarytuberculosiswithbloodeosinophilia AT yuvkolobovnikova featuresofimmunoregulationinpatientswithpulmonarytuberculosiswithbloodeosinophilia AT vvnovitskiy featuresofimmunoregulationinpatientswithpulmonarytuberculosiswithbloodeosinophilia AT avkaraulov featuresofimmunoregulationinpatientswithpulmonarytuberculosiswithbloodeosinophilia AT elnikulina featuresofimmunoregulationinpatientswithpulmonarytuberculosiswithbloodeosinophilia AT vspoletika featuresofimmunoregulationinpatientswithpulmonarytuberculosiswithbloodeosinophilia |