A comparison between microscopical and ELISA technique in diagnosis of Giardia lamblia infection with epidemiological and biochemical study among Children under ten years in Tikrit District

The current study includes 644 stool samples of patients infected with diarrhea less than ten years old who are attended Tikrit Teaching Hospital from 1/12/2008 to 30/6/2009. Three methods are used for the examination of stool, the first one is the direct microscopical test. The number of samples w...

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Main Authors: Sinai Najy Muhsin, Ibrahim Sh. Daoud
Format: Article
Language:English
Published: Tikrit University 2023-02-01
Series:Tikrit Journal of Pure Science
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Online Access:https://tjpsj.org/index.php/tjps/article/view/1188
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author Sinai Najy Muhsin
Ibrahim Sh. Daoud
author_facet Sinai Najy Muhsin
Ibrahim Sh. Daoud
author_sort Sinai Najy Muhsin
collection DOAJ
description The current study includes 644 stool samples of patients infected with diarrhea less than ten years old who are attended Tikrit Teaching Hospital from 1/12/2008 to 30/6/2009. Three methods are used for the examination of stool, the first one is the direct microscopical test. The number of samples which are tested are 55 with the percentage of 8.54%. The second is the floatation method with three solution[ Zinc Sulphate solution, Sucrose solution, and Sodium chloride solution] The number of samples which are tested by floatation method are 64 with the percentage of 9.94%. The third is ELISA method to detect parasite antigen in stool. The number of samples which are tested by this methods are 80 with the percentage of 12.42%. The results show there are significant difference between infected patients  with giardiasis and the healthy persons group and for significant level 0.05<p in the mentioned tests. The study points out a decrease in both Hb, PCV and an increase in the rate of  WBCc and ESR . The study point out a decrease in the rate of Ca++,  Mg++, Na+, Tp, Chol. And Tri.  and an increase in the rate concentration of GOT, GPT.  
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spelling doaj-art-5b9c0a8d2db745b9b7cd6bcf7cd8eeb12025-07-11T15:32:47ZengTikrit UniversityTikrit Journal of Pure Science1813-16622415-17262023-02-0120310.25130/tjps.v20i3.1188A comparison between microscopical and ELISA technique in diagnosis of Giardia lamblia infection with epidemiological and biochemical study among Children under ten years in Tikrit DistrictSinai Najy MuhsinIbrahim Sh. Daoud The current study includes 644 stool samples of patients infected with diarrhea less than ten years old who are attended Tikrit Teaching Hospital from 1/12/2008 to 30/6/2009. Three methods are used for the examination of stool, the first one is the direct microscopical test. The number of samples which are tested are 55 with the percentage of 8.54%. The second is the floatation method with three solution[ Zinc Sulphate solution, Sucrose solution, and Sodium chloride solution] The number of samples which are tested by floatation method are 64 with the percentage of 9.94%. The third is ELISA method to detect parasite antigen in stool. The number of samples which are tested by this methods are 80 with the percentage of 12.42%. The results show there are significant difference between infected patients  with giardiasis and the healthy persons group and for significant level 0.05<p in the mentioned tests. The study points out a decrease in both Hb, PCV and an increase in the rate of  WBCc and ESR . The study point out a decrease in the rate of Ca++,  Mg++, Na+, Tp, Chol. And Tri.  and an increase in the rate concentration of GOT, GPT.   https://tjpsj.org/index.php/tjps/article/view/1188comparisonmicroscopicalELISA techniqueGiardia lamblia infectionbiochemical study
spellingShingle Sinai Najy Muhsin
Ibrahim Sh. Daoud
A comparison between microscopical and ELISA technique in diagnosis of Giardia lamblia infection with epidemiological and biochemical study among Children under ten years in Tikrit District
Tikrit Journal of Pure Science
comparison
microscopical
ELISA technique
Giardia lamblia infection
biochemical study
title A comparison between microscopical and ELISA technique in diagnosis of Giardia lamblia infection with epidemiological and biochemical study among Children under ten years in Tikrit District
title_full A comparison between microscopical and ELISA technique in diagnosis of Giardia lamblia infection with epidemiological and biochemical study among Children under ten years in Tikrit District
title_fullStr A comparison between microscopical and ELISA technique in diagnosis of Giardia lamblia infection with epidemiological and biochemical study among Children under ten years in Tikrit District
title_full_unstemmed A comparison between microscopical and ELISA technique in diagnosis of Giardia lamblia infection with epidemiological and biochemical study among Children under ten years in Tikrit District
title_short A comparison between microscopical and ELISA technique in diagnosis of Giardia lamblia infection with epidemiological and biochemical study among Children under ten years in Tikrit District
title_sort comparison between microscopical and elisa technique in diagnosis of giardia lamblia infection with epidemiological and biochemical study among children under ten years in tikrit district
topic comparison
microscopical
ELISA technique
Giardia lamblia infection
biochemical study
url https://tjpsj.org/index.php/tjps/article/view/1188
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