Simultaneous Quantification of Main Saponins in <i>Panax vietnamensis</i> by HPLC-PDA/ELSD Using the Quantitative Analysis of Multi-Components by Single-Marker Method
<b>Background</b>: The Quantitative Analysis of Multi-components by Single-marker (QAMS) method has been developed as an alternative to the External Standards Method (ESM) for the quality control of medicinal herbs. <b>Objectives</b>: In this study, QAMS was developed to dete...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-06-01
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| Series: | Metabolites |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-1989/15/7/419 |
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| Summary: | <b>Background</b>: The Quantitative Analysis of Multi-components by Single-marker (QAMS) method has been developed as an alternative to the External Standards Method (ESM) for the quality control of medicinal herbs. <b>Objectives</b>: In this study, QAMS was developed to determine saponins in the raw materials of <i>Panax vietnamensis</i> using HPLC-PDA/ELSD. <b>Methods</b>: The method was developed and validated. The relative conversion factors F<sub>x</sub> were calculated based on the linear regression for HPLC-PDA and the logarithm equation for HPLC-ELSD. The Standard Method Difference (SMD) was determined to indicate the difference in the results of QAMS and EMS. <b>Results</b>: Relative conversion factors (F<sub>x</sub>) were determined for each detector to quantify five saponins (ginsenoside Rb<sub>1</sub>, Rd, Rg<sub>1</sub>, majnoside R2, and vina-ginsenoside R2) in VG root. The F<sub>x</sub> values were calculated based on the ratio of the slopes of the regression equations of a single standard and an external standard. For HPLC-PDA, G-Rb1 was used as a single standard with the F<sub>x</sub> values of 1.00 (G-Rb<sub>1</sub>), 1.08 (G-Rd), 1.32 (G-Rg<sub>1</sub>), and 0.04 (M-R2). For HPLC-ELSD, G-Rb<sub>1</sub> was used for determining the content of G-Rg<sub>1</sub> and G-Rb<sub>1</sub> with the F<sub>x</sub> values of 1.00 (G-Rb<sub>1</sub>) and 0.95 (G-Rg<sub>1</sub>), while M-R2 was used for quantitating M-R2 and V-R2 with F<sub>x</sub> of 1.00 (M-R2) and 1.05 (V-R2). An SMD value less than 5.00% confirms the close alignment of the QAMS method with ESM. <b>Conclusions</b>: The QAMS method proved to be a feasible and promising method for the quality control of <i>P. vietnamensis</i>. |
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| ISSN: | 2218-1989 |