Generation of recombinant rabies virus ERA strain applied to virus tracking in cell infection

Generation of recombinant rabies virus ERA strain applied to virus tracking in cell infection

The mechanism of rabies virus (RABV) infection still needs to be further characterized. RABV particle with self-fluorescent is a powerful viral model to visualize the viral infection process in cells. Herein, based on a reverse genetic system of the Evelyn-Rokitnicki-Abelseth (rERA) strain, we gener...

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Hauptverfasser: Dan-dan ZHAO, Lei SHUAI, Jin-ying GE, Jin-liang WANG, Zhi-yuan WEN, Ren-qiang LIU, Chong WANG, Xi-jun WANG, Zhi-gao BU
Format: Artikel
Sprache:Englisch
Veröffentlicht: KeAi Communications Co., Ltd. 2019-10-01
Schriftenreihe:Journal of Integrative Agriculture
Schlagworte:
rabies virus
self-fluorescence
binding
internalization
Online-Zugang:http://www.sciencedirect.com/science/article/pii/S2095311919627176
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Zusammenfassung:The mechanism of rabies virus (RABV) infection still needs to be further characterized. RABV particle with self-fluorescent is a powerful viral model to visualize the viral infection process in cells. Herein, based on a reverse genetic system of the Evelyn-Rokitnicki-Abelseth (rERA) strain, we generated a recombinant RABV rERA-N/mCherry strain that stably expresses an additional ERA nucleoprotein that fuses with the red fluorescent protein mCherry (N/mCherry). The rERA-N/mCherry strain retained growth property similar to the parent strain rERA in vitro. The N/mCherry expression showed genetic stability during passage into mouse neuroblastoma (NA) cells and did not change the virulence of the vector. The rERA-N/mCherry strain was then utilized as a visual viral model to study the RABV-cell binding and internalization. We directly observed the red self-fluorescence of rERA-N/mCherry particles binding to the cell surface, and further co-localizing with clathrin in the early stage of infection in NA cells by fluorescence microscopy. Our results showed that the rERA-N/mCherry strain uses clathrin-dependent endocytosis to enter cells, which is consistent with the well-known mechanism of RABV invasion. The recombinant RABV rERA-N/mCherry thus appears to have the potential to be an effective viral model to further explore the fundamental molecular mechanism of rabies neuropathogenesis.
ISSN:2095-3119

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