Defining Keypoints to Align H&E Images and Xenium DAPI-Stained Images Automatically

Defining Keypoints to Align H&E Images and Xenium DAPI-Stained Images Automatically

10X Xenium is an in situ spatial transcriptomics platform that enables single-cell and subcellular-level gene expression analysis. In Xenium data analysis, defining matched keypoints to align H&E and spatial transcriptomic images is critical for cross-referencing sequencing and histology. Curren...

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Bibliographic Details
Main Authors: Yu Lin, Yan Wang, Juexin Wang, Mauminah Raina, Ricardo Melo Ferreira, Michael T. Eadon, Yanchun Liang, Dong Xu
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Cells
Subjects:
spatial transcriptomics
Xenium technology
H&E image
image alignment
nucleus segmentation
graph matching
Online Access:https://www.mdpi.com/2073-4409/14/13/1000
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