Pathogen reduction diminishes the protective effects of platelets on endothelial barrier permeability in vitro
Abstract: Platelet transfusion not only attenuates bleeding and promotes hemostasis but also plays a critical role in vascular stability and endothelial barrier integrity. Under amotosalen-UVA pathogen reduction of platelets, pathogen nucleic acids undergo adduction, which prevents their replication...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-08-01
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| Series: | Blood Vessels, Thrombosis & Hemostasis |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2950327225000154 |
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| author | Alison B. Nair Byron Miyazawa Alpa Trivedi Lindsay Vivona Alexander Fields Kimberly Herrera Lucy Z. Kornblith Joseph Cuschieri Shibani Pati |
| author_facet | Alison B. Nair Byron Miyazawa Alpa Trivedi Lindsay Vivona Alexander Fields Kimberly Herrera Lucy Z. Kornblith Joseph Cuschieri Shibani Pati |
| author_sort | Alison B. Nair |
| collection | DOAJ |
| description | Abstract: Platelet transfusion not only attenuates bleeding and promotes hemostasis but also plays a critical role in vascular stability and endothelial barrier integrity. Under amotosalen-UVA pathogen reduction of platelets, pathogen nucleic acids undergo adduction, which prevents their replication and greatly reduces the risk of transfusion-transmitted infections. Although pathogen-reduced (PR) platelets are increasing in clinical use, the physiologic effects of pathogen reduction on platelets, particularly its impact on platelet-endothelial interactions, have yet to be described. This study compared PR platelets with nonpathogen-reduced (NPR) platelets in measures of effect on endothelial permeability in vitro. We hypothesized that PR platelets would be similar to NPR platelets. However, in endothelial cell immunohistochemistry and impedance assays, PR platelets demonstrated a significantly diminished capacity to attenuate endothelial barrier permeability at early storage time points. This small but significant difference requires further mechanistic and clinical study to understand its implications, particularly in patients with bleeding with vascular fragility. |
| format | Article |
| id | doaj-art-54d93e6fb98c4648a89d5cd212e9a48c |
| institution | Matheson Library |
| issn | 2950-3272 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Blood Vessels, Thrombosis & Hemostasis |
| spelling | doaj-art-54d93e6fb98c4648a89d5cd212e9a48c2025-08-01T04:45:33ZengElsevierBlood Vessels, Thrombosis & Hemostasis2950-32722025-08-0123100058Pathogen reduction diminishes the protective effects of platelets on endothelial barrier permeability in vitroAlison B. Nair0Byron Miyazawa1Alpa Trivedi2Lindsay Vivona3Alexander Fields4Kimberly Herrera5Lucy Z. Kornblith6Joseph Cuschieri7Shibani Pati8Department of Pediatrics, University of California, San Francisco, CA; Correspondence: Alison B. Nair, Department of Pediatrics, University of California, San Francisco, Mission Hall 550 16th St, San Francisco, CA 94158;Department of Laboratory Medicine, University of California, San Francisco, CADepartment of Laboratory Medicine, University of California, San Francisco, CADepartment of Laboratory Medicine, University of California, San Francisco, CA; Department of Biological Sciences, University of Illinois, Chicago, ILDepartment of Surgery, University of California, San Francisco, CADepartment of Surgery, University of California, San Francisco, CA; Department of Rheumatology, Cedars-Sinai Medical Center, Los Angeles, CADepartment of Laboratory Medicine, University of California, San Francisco, CA; Department of Surgery, University of California, San Francisco, CADepartment of Laboratory Medicine, University of California, San Francisco, CA; Department of Surgery, University of California, San Francisco, CADepartment of Laboratory Medicine, University of California, San Francisco, CA; Department of Surgery, University of California, San Francisco, CA; Shibani Pati, Department of Laboratory Medicine and Department of Surgery, University of California San Francisco, 513 Parnassus Ave, HSE-715, San Francisco, CA 94143;Abstract: Platelet transfusion not only attenuates bleeding and promotes hemostasis but also plays a critical role in vascular stability and endothelial barrier integrity. Under amotosalen-UVA pathogen reduction of platelets, pathogen nucleic acids undergo adduction, which prevents their replication and greatly reduces the risk of transfusion-transmitted infections. Although pathogen-reduced (PR) platelets are increasing in clinical use, the physiologic effects of pathogen reduction on platelets, particularly its impact on platelet-endothelial interactions, have yet to be described. This study compared PR platelets with nonpathogen-reduced (NPR) platelets in measures of effect on endothelial permeability in vitro. We hypothesized that PR platelets would be similar to NPR platelets. However, in endothelial cell immunohistochemistry and impedance assays, PR platelets demonstrated a significantly diminished capacity to attenuate endothelial barrier permeability at early storage time points. This small but significant difference requires further mechanistic and clinical study to understand its implications, particularly in patients with bleeding with vascular fragility.http://www.sciencedirect.com/science/article/pii/S2950327225000154 |
| spellingShingle | Alison B. Nair Byron Miyazawa Alpa Trivedi Lindsay Vivona Alexander Fields Kimberly Herrera Lucy Z. Kornblith Joseph Cuschieri Shibani Pati Pathogen reduction diminishes the protective effects of platelets on endothelial barrier permeability in vitro Blood Vessels, Thrombosis & Hemostasis |
| title | Pathogen reduction diminishes the protective effects of platelets on endothelial barrier permeability in vitro |
| title_full | Pathogen reduction diminishes the protective effects of platelets on endothelial barrier permeability in vitro |
| title_fullStr | Pathogen reduction diminishes the protective effects of platelets on endothelial barrier permeability in vitro |
| title_full_unstemmed | Pathogen reduction diminishes the protective effects of platelets on endothelial barrier permeability in vitro |
| title_short | Pathogen reduction diminishes the protective effects of platelets on endothelial barrier permeability in vitro |
| title_sort | pathogen reduction diminishes the protective effects of platelets on endothelial barrier permeability in vitro |
| url | http://www.sciencedirect.com/science/article/pii/S2950327225000154 |
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