Cloning and Expression of <i>ace</i> Gene of <i>Vibrio cholerae</i> in <i>Escherichia coli</i>

Cloning and Expression of <i>ace</i> Gene of <i>Vibrio cholerae</i> in <i>Escherichia coli</i>

Accessory cholera enterotoxin (ace) gene of Vibrio cholerae, amplified by PCR, is cloned into the BamHI-HindIII sites using plasmid pQE30. Expression of ace gene is under the control of T5 promotor. The Escherichia coli strain M15[pREP4]pAce90 (KM 194), containing recombinant plasmid, is an active p...

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Bibliographic Details
Main Authors: E. V. Monakhova, R. V. Pisanov, L. M. Verkina, A. V. Mironova
Format: Article
Language:Russian
Published: Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” 2012-04-01
Series:Проблемы особо опасных инфекций
Subjects:
accessory cholera enterotoxin
cholera vibrio
gene cloning
recombinant plasmid
Online Access:https://journal.microbe.ru/jour/article/view/792
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