Follow-Up of the Immune Response and the Possible Presence of <i>Brucella melitensis</i> Strains in Peripheral Blood in Hoggets Vaccinated by Rev1 in Greece
Brucellosis in small ruminants, primarily caused by <i>B. melitensis</i>, remains a significant threat to public health in many regions. Although early-age vaccination of breeding stocks was expected to facilitate infection control, this approach did not meet expectations, while in some...
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2025-06-01
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| author | Maria Babetsa Hanka Brangsch Gamal Wareth Ilias Bouzalas Athanasios I. Gelasakis Antonios Zdragas Loukia V. Ekateriniadou Evridiki Boukouvala Athanasios I. Papadopoulos |
| author_facet | Maria Babetsa Hanka Brangsch Gamal Wareth Ilias Bouzalas Athanasios I. Gelasakis Antonios Zdragas Loukia V. Ekateriniadou Evridiki Boukouvala Athanasios I. Papadopoulos |
| author_sort | Maria Babetsa |
| collection | DOAJ |
| description | Brucellosis in small ruminants, primarily caused by <i>B. melitensis</i>, remains a significant threat to public health in many regions. Although early-age vaccination of breeding stocks was expected to facilitate infection control, this approach did not meet expectations, while in some cases, late vaccination of animals has been associated with an increased number of human cases. Therefore, in this field study, we investigated the immune response and bacteremia cases in ten apparently healthy hoggets vaccinated at the age of nine months. Before vaccination, the hoggets were seronegative and negative in blood cultures, although <i>B. melitensis</i> DNA was detected in three animals using PCR. After vaccination, twelve <i>Brucella</i> spp. strains were isolated from the blood cultures of nine hoggets at different time points. Whole genome analysis identified eleven of them as identical to three <i>B. melitensis</i> strains previously isolated in Greece. The tested animals completed their gestation without any adverse outcomes. According to our results, late vaccination, despite extending animal exposure to <i>B. melitensis</i>, apparently protects against disease and abortion but not against infection. The onset of post-vaccination immune response may be influenced by transient infections by field strains. |
| format | Article |
| id | doaj-art-519d12a7bfed4a94a97a33a9bb3618c7 |
| institution | Matheson Library |
| issn | 2036-7481 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
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| series | Microbiology Research |
| spelling | doaj-art-519d12a7bfed4a94a97a33a9bb3618c72025-06-25T14:11:18ZengMDPI AGMicrobiology Research2036-74812025-06-0116612410.3390/microbiolres16060124Follow-Up of the Immune Response and the Possible Presence of <i>Brucella melitensis</i> Strains in Peripheral Blood in Hoggets Vaccinated by Rev1 in GreeceMaria Babetsa0Hanka Brangsch1Gamal Wareth2Ilias Bouzalas3Athanasios I. Gelasakis4Antonios Zdragas5Loukia V. Ekateriniadou6Evridiki Boukouvala7Athanasios I. Papadopoulos8ELGO-DIMITRA, Veterinary Research Institute, Campus of Thermi, 57001 Thessaloniki, GreeceInstitute of Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institut (FLI), 07743 Jena, GermanyInstitute of Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institut (FLI), 07743 Jena, GermanyELGO-DIMITRA, Veterinary Research Institute, Campus of Thermi, 57001 Thessaloniki, GreeceLaboratory of Anatomy and Physiology of Farm Animals, Department of Animal Science, School of Animal Biosciences, Agricultural University of Athens, Iera Odos 75 Str., 11855 Athens, GreeceELGO-DIMITRA, Veterinary Research Institute, Campus of Thermi, 57001 Thessaloniki, GreeceELGO-DIMITRA, Veterinary Research Institute, Campus of Thermi, 57001 Thessaloniki, GreeceELGO-DIMITRA, Veterinary Research Institute, Campus of Thermi, 57001 Thessaloniki, GreeceLaboratory of Animal Physiology, Department of Zoology, School of Biology, Faculty of Science, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceBrucellosis in small ruminants, primarily caused by <i>B. melitensis</i>, remains a significant threat to public health in many regions. Although early-age vaccination of breeding stocks was expected to facilitate infection control, this approach did not meet expectations, while in some cases, late vaccination of animals has been associated with an increased number of human cases. Therefore, in this field study, we investigated the immune response and bacteremia cases in ten apparently healthy hoggets vaccinated at the age of nine months. Before vaccination, the hoggets were seronegative and negative in blood cultures, although <i>B. melitensis</i> DNA was detected in three animals using PCR. After vaccination, twelve <i>Brucella</i> spp. strains were isolated from the blood cultures of nine hoggets at different time points. Whole genome analysis identified eleven of them as identical to three <i>B. melitensis</i> strains previously isolated in Greece. The tested animals completed their gestation without any adverse outcomes. According to our results, late vaccination, despite extending animal exposure to <i>B. melitensis</i>, apparently protects against disease and abortion but not against infection. The onset of post-vaccination immune response may be influenced by transient infections by field strains.https://www.mdpi.com/2036-7481/16/6/124<i>Brucella melitensis</i>Rev1blood culturesPCRfield strainsWGS |
| spellingShingle | Maria Babetsa Hanka Brangsch Gamal Wareth Ilias Bouzalas Athanasios I. Gelasakis Antonios Zdragas Loukia V. Ekateriniadou Evridiki Boukouvala Athanasios I. Papadopoulos Follow-Up of the Immune Response and the Possible Presence of <i>Brucella melitensis</i> Strains in Peripheral Blood in Hoggets Vaccinated by Rev1 in Greece Microbiology Research <i>Brucella melitensis</i> Rev1 blood cultures PCR field strains WGS |
| title | Follow-Up of the Immune Response and the Possible Presence of <i>Brucella melitensis</i> Strains in Peripheral Blood in Hoggets Vaccinated by Rev1 in Greece |
| title_full | Follow-Up of the Immune Response and the Possible Presence of <i>Brucella melitensis</i> Strains in Peripheral Blood in Hoggets Vaccinated by Rev1 in Greece |
| title_fullStr | Follow-Up of the Immune Response and the Possible Presence of <i>Brucella melitensis</i> Strains in Peripheral Blood in Hoggets Vaccinated by Rev1 in Greece |
| title_full_unstemmed | Follow-Up of the Immune Response and the Possible Presence of <i>Brucella melitensis</i> Strains in Peripheral Blood in Hoggets Vaccinated by Rev1 in Greece |
| title_short | Follow-Up of the Immune Response and the Possible Presence of <i>Brucella melitensis</i> Strains in Peripheral Blood in Hoggets Vaccinated by Rev1 in Greece |
| title_sort | follow up of the immune response and the possible presence of i brucella melitensis i strains in peripheral blood in hoggets vaccinated by rev1 in greece |
| topic | <i>Brucella melitensis</i> Rev1 blood cultures PCR field strains WGS |
| url | https://www.mdpi.com/2036-7481/16/6/124 |
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