Optimization of Pectinase Production from Silkworm Excrement Using <i>Aspergillus niger</i>
Silkworm excrement, a byproduct of the sericulture industry, is rich in organic compounds and presents a sustainable substrate for enzyme production. This study investigates the potential of silkworm excrement as a substrate for pectinase production using <i>Aspergillus niger</i> submerg...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-06-01
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| Series: | Fermentation |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-5637/11/6/333 |
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| Summary: | Silkworm excrement, a byproduct of the sericulture industry, is rich in organic compounds and presents a sustainable substrate for enzyme production. This study investigates the potential of silkworm excrement as a substrate for pectinase production using <i>Aspergillus niger</i> submerged fermentation. Single-factor experiments and a Box–Behnken Design (BBD) were employed to identify and optimize the key medium components and their interactions. Results indicated that the optimal concentrations for maximum pectinase activity were CaCl<sub>2</sub> at 0.437 g/L, orange peel powder at 4.223 g/L, (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub> at 15.761 g/L, and bran at 33.590 g/L. The optimized conditions achieved a pectinase activity of 20.505 U/mL, validating the robustness of the RSM model. This approach not only maximizes enzyme activity but also presents a sustainable and cost-effective method for utilizing a byproduct in the sericulture industry, aligning with principles of circular economy and waste minimization. |
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| ISSN: | 2311-5637 |