β-Catenin transcriptional activity is required for establishment of inner pillar cell identity during cochlear development.
The mammalian cochlea is composed of sensory hair cells as well as multiple different types of non-sensory supporting cells. Pillar cells are one type of supporting cell that form the tunnel of Corti and include two morphologically and functionally distinct subtypes: inner pillar cells (IPCs) and ou...
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Public Library of Science (PLoS)
2023-08-01
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| Series: | PLoS Genetics |
| Online Access: | https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1010925&type=printable |
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| author | Michael Ebeid Ippei Kishimoto Pooja Roy Mohd Ali Abbas Zaidi Alan G Cheng Sung-Ho Huh |
| author_facet | Michael Ebeid Ippei Kishimoto Pooja Roy Mohd Ali Abbas Zaidi Alan G Cheng Sung-Ho Huh |
| author_sort | Michael Ebeid |
| collection | DOAJ |
| description | The mammalian cochlea is composed of sensory hair cells as well as multiple different types of non-sensory supporting cells. Pillar cells are one type of supporting cell that form the tunnel of Corti and include two morphologically and functionally distinct subtypes: inner pillar cells (IPCs) and outer pillar cells (OPCs). The processes of specification and differentiation of inner versus outer pillar cells are still unclear. Here, we show that β-Catenin is required for establishing IPC identity in the mammalian cochlea. To differentiate the transcriptional and adhesion roles of β-Catenin in establishing IPC identity, we examined two different models of β-Catenin deletion; one that deletes both transcriptional and structural functions and one which retains cell adhesion function but lacks transcriptional function. Here, we show that cochleae lacking β-Catenin transcriptional function lost IPCs and displayed extranumerary OPCs, indicating its requirement for establishing IPC identity. Overexpression of β-Catenin induced proliferation within IPCs but not ectopic IPCs. Single-cell transcriptomes of supporting cells lacking β-Catenin transcriptional function show a loss of the IPC and gain of OPC signatures. Finally, targeted deletion of β-Catenin in IPCs also led to the loss of IPC identity, indicating a cell autonomous role of β-Catenin in establishing IPC identity. As IPCs have the capacity to regenerate sensory hair cells in the postnatal cochlea, our results will aid in future IPC-based hair cell regeneration strategies. |
| format | Article |
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| institution | Matheson Library |
| issn | 1553-7390 1553-7404 |
| language | English |
| publishDate | 2023-08-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj-art-4eaa9e2f51a642d1a8ee9f3c648feafd2025-07-25T05:31:06ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042023-08-01198e101092510.1371/journal.pgen.1010925β-Catenin transcriptional activity is required for establishment of inner pillar cell identity during cochlear development.Michael EbeidIppei KishimotoPooja RoyMohd Ali Abbas ZaidiAlan G ChengSung-Ho HuhThe mammalian cochlea is composed of sensory hair cells as well as multiple different types of non-sensory supporting cells. Pillar cells are one type of supporting cell that form the tunnel of Corti and include two morphologically and functionally distinct subtypes: inner pillar cells (IPCs) and outer pillar cells (OPCs). The processes of specification and differentiation of inner versus outer pillar cells are still unclear. Here, we show that β-Catenin is required for establishing IPC identity in the mammalian cochlea. To differentiate the transcriptional and adhesion roles of β-Catenin in establishing IPC identity, we examined two different models of β-Catenin deletion; one that deletes both transcriptional and structural functions and one which retains cell adhesion function but lacks transcriptional function. Here, we show that cochleae lacking β-Catenin transcriptional function lost IPCs and displayed extranumerary OPCs, indicating its requirement for establishing IPC identity. Overexpression of β-Catenin induced proliferation within IPCs but not ectopic IPCs. Single-cell transcriptomes of supporting cells lacking β-Catenin transcriptional function show a loss of the IPC and gain of OPC signatures. Finally, targeted deletion of β-Catenin in IPCs also led to the loss of IPC identity, indicating a cell autonomous role of β-Catenin in establishing IPC identity. As IPCs have the capacity to regenerate sensory hair cells in the postnatal cochlea, our results will aid in future IPC-based hair cell regeneration strategies.https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1010925&type=printable |
| spellingShingle | Michael Ebeid Ippei Kishimoto Pooja Roy Mohd Ali Abbas Zaidi Alan G Cheng Sung-Ho Huh β-Catenin transcriptional activity is required for establishment of inner pillar cell identity during cochlear development. PLoS Genetics |
| title | β-Catenin transcriptional activity is required for establishment of inner pillar cell identity during cochlear development. |
| title_full | β-Catenin transcriptional activity is required for establishment of inner pillar cell identity during cochlear development. |
| title_fullStr | β-Catenin transcriptional activity is required for establishment of inner pillar cell identity during cochlear development. |
| title_full_unstemmed | β-Catenin transcriptional activity is required for establishment of inner pillar cell identity during cochlear development. |
| title_short | β-Catenin transcriptional activity is required for establishment of inner pillar cell identity during cochlear development. |
| title_sort | β catenin transcriptional activity is required for establishment of inner pillar cell identity during cochlear development |
| url | https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1010925&type=printable |
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