Functional analysis of tomato MAP65 gene family, highlighting SlMAP65-1’s role in fruit morphogenesis
The plant growth process is accompanied by dynamic changes in the microtubules, and the rearrangement of microtubules is regulated by diverse microtubule-associated proteins (MAPs). Plant MAP65s have been exhaustively characterized in some species, but the information about MAP65 family members in t...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2025-02-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311924002570 |
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| author | Peiyu Zhang Guoning Zhu Chunjiao Zhang Hongliang Zhu |
| author_facet | Peiyu Zhang Guoning Zhu Chunjiao Zhang Hongliang Zhu |
| author_sort | Peiyu Zhang |
| collection | DOAJ |
| description | The plant growth process is accompanied by dynamic changes in the microtubules, and the rearrangement of microtubules is regulated by diverse microtubule-associated proteins (MAPs). Plant MAP65s have been exhaustively characterized in some species, but the information about MAP65 family members in tomato (Solanum lycopersicum) is limited. In this study, nine SlMAP65 family genes were identified in the tomato genome. Then a systematic analysis that considered the physio-chemical properties, evolution, conserved motifs, domains, gene structure, and cis-regulatory elements of SlMAP65 family members was conducted. The family member SlMAP65-1, which had the highest expression, was knocked out by CRISPR/Cas9. The tomato fruit of slmap65-1 loss of function lines showed an elongated morphology, and the data indicated that SlMAP65-1 is involved in fruit morphogenesis at the early fruit development stage. These results provide new insights for fruit morphogenesis-related research and future functional studies of the SlMAP65 family members in tomato. |
| format | Article |
| id | doaj-art-4ea97c53eabe4ed18d1a56a9233c4501 |
| institution | Matheson Library |
| issn | 2095-3119 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-4ea97c53eabe4ed18d1a56a9233c45012025-08-02T07:35:41ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192025-02-01242564574Functional analysis of tomato MAP65 gene family, highlighting SlMAP65-1’s role in fruit morphogenesisPeiyu Zhang0Guoning Zhu1Chunjiao Zhang2Hongliang Zhu3College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaSupervision, Inspection & Testing Center of Agricultural Products Quality, Ministry of Agriculture and Rural Affairs, Beijing 100083, China; Correspondence Chunjiao Zhang, Hongliang ZhuCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; Correspondence Chunjiao Zhang, Hongliang ZhuThe plant growth process is accompanied by dynamic changes in the microtubules, and the rearrangement of microtubules is regulated by diverse microtubule-associated proteins (MAPs). Plant MAP65s have been exhaustively characterized in some species, but the information about MAP65 family members in tomato (Solanum lycopersicum) is limited. In this study, nine SlMAP65 family genes were identified in the tomato genome. Then a systematic analysis that considered the physio-chemical properties, evolution, conserved motifs, domains, gene structure, and cis-regulatory elements of SlMAP65 family members was conducted. The family member SlMAP65-1, which had the highest expression, was knocked out by CRISPR/Cas9. The tomato fruit of slmap65-1 loss of function lines showed an elongated morphology, and the data indicated that SlMAP65-1 is involved in fruit morphogenesis at the early fruit development stage. These results provide new insights for fruit morphogenesis-related research and future functional studies of the SlMAP65 family members in tomato.http://www.sciencedirect.com/science/article/pii/S2095311924002570tomatoMAP65 familyfruit shapemicrotubule organization |
| spellingShingle | Peiyu Zhang Guoning Zhu Chunjiao Zhang Hongliang Zhu Functional analysis of tomato MAP65 gene family, highlighting SlMAP65-1’s role in fruit morphogenesis Journal of Integrative Agriculture tomato MAP65 family fruit shape microtubule organization |
| title | Functional analysis of tomato MAP65 gene family, highlighting SlMAP65-1’s role in fruit morphogenesis |
| title_full | Functional analysis of tomato MAP65 gene family, highlighting SlMAP65-1’s role in fruit morphogenesis |
| title_fullStr | Functional analysis of tomato MAP65 gene family, highlighting SlMAP65-1’s role in fruit morphogenesis |
| title_full_unstemmed | Functional analysis of tomato MAP65 gene family, highlighting SlMAP65-1’s role in fruit morphogenesis |
| title_short | Functional analysis of tomato MAP65 gene family, highlighting SlMAP65-1’s role in fruit morphogenesis |
| title_sort | functional analysis of tomato map65 gene family highlighting slmap65 1 s role in fruit morphogenesis |
| topic | tomato MAP65 family fruit shape microtubule organization |
| url | http://www.sciencedirect.com/science/article/pii/S2095311924002570 |
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