Quantitative Phase Imaging During Cell Division Based on Dual-Channel Microscopic Interferometry System
Quantitative phase imaging (QPI) during cell division is implemented by ourhomemade dual-channel microscopic interferometry (DCMI) system. A pair of interferograms with a fixed phase shift of π/2 is simultaneously captured by the DCMI system, and two-step phase demodulation alg...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
IEEE
2020-01-01
|
| Series: | IEEE Photonics Journal |
| Subjects: | |
| Online Access: | https://ieeexplore.ieee.org/document/8924630/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1839644850404720640 |
|---|---|
| author | Shengde Liu Dejin Zheng Jiaosheng Li Jindong Tian Liyun Zhong Xiaoxu Lu |
| author_facet | Shengde Liu Dejin Zheng Jiaosheng Li Jindong Tian Liyun Zhong Xiaoxu Lu |
| author_sort | Shengde Liu |
| collection | DOAJ |
| description | Quantitative phase imaging (QPI) during cell division is implemented by ourhomemade dual-channel microscopic interferometry (DCMI) system. A pair of interferograms with a fixed phase shift of π/2 is simultaneously captured by the DCMI system, and two-step phase demodulation algorithm is employed for phase retrieval. By capturing a sequence of paired-interferograms with the DCMI system, we achieve the dynamic QPI during cell division, and then the cellular surface area, volume and the ratio of surface area to volume (RSV) and their variations. Both the reliability and stability of the DCMI system are verified. In addition to maintaining the advantages of optical interferometry, this DCMI system is very suitable for dynamic QPI due to its rapid speed of phase retrieval. Importantly, this DCMI based QPI method will supply a powerful tool for studying the precise mechanism during cell division, differentiation, apoptosis and other dynamic processes. |
| format | Article |
| id | doaj-art-4e8d6dc1a23e4c86a40040f8fe5c75a4 |
| institution | Matheson Library |
| issn | 1943-0655 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | IEEE |
| record_format | Article |
| series | IEEE Photonics Journal |
| spelling | doaj-art-4e8d6dc1a23e4c86a40040f8fe5c75a42025-07-01T23:46:10ZengIEEEIEEE Photonics Journal1943-06552020-01-0112111110.1109/JPHOT.2019.29557518924630Quantitative Phase Imaging During Cell Division Based on Dual-Channel Microscopic Interferometry SystemShengde Liu0https://orcid.org/0000-0002-1024-6843Dejin Zheng1Jiaosheng Li2https://orcid.org/0000-0001-7517-4177Jindong Tian3https://orcid.org/0000-0002-0334-1762Liyun Zhong4Xiaoxu Lu5https://orcid.org/0000-0001-6757-6061Guangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices, South China Normal University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices, South China Normal University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices, South China Normal University, Guangzhou, ChinaShenzhen Key Laboratory of Micro-Nano Measuring and Imaging in Biomedical Optics, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, ChinaGuangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices, South China Normal University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices, South China Normal University, Guangzhou, ChinaQuantitative phase imaging (QPI) during cell division is implemented by ourhomemade dual-channel microscopic interferometry (DCMI) system. A pair of interferograms with a fixed phase shift of π/2 is simultaneously captured by the DCMI system, and two-step phase demodulation algorithm is employed for phase retrieval. By capturing a sequence of paired-interferograms with the DCMI system, we achieve the dynamic QPI during cell division, and then the cellular surface area, volume and the ratio of surface area to volume (RSV) and their variations. Both the reliability and stability of the DCMI system are verified. In addition to maintaining the advantages of optical interferometry, this DCMI system is very suitable for dynamic QPI due to its rapid speed of phase retrieval. Importantly, this DCMI based QPI method will supply a powerful tool for studying the precise mechanism during cell division, differentiation, apoptosis and other dynamic processes.https://ieeexplore.ieee.org/document/8924630/Quantitative phase imagingdual-channel microscopic interferometryphase shiftcell division. |
| spellingShingle | Shengde Liu Dejin Zheng Jiaosheng Li Jindong Tian Liyun Zhong Xiaoxu Lu Quantitative Phase Imaging During Cell Division Based on Dual-Channel Microscopic Interferometry System IEEE Photonics Journal Quantitative phase imaging dual-channel microscopic interferometry phase shift cell division. |
| title | Quantitative Phase Imaging During Cell Division Based on Dual-Channel Microscopic Interferometry System |
| title_full | Quantitative Phase Imaging During Cell Division Based on Dual-Channel Microscopic Interferometry System |
| title_fullStr | Quantitative Phase Imaging During Cell Division Based on Dual-Channel Microscopic Interferometry System |
| title_full_unstemmed | Quantitative Phase Imaging During Cell Division Based on Dual-Channel Microscopic Interferometry System |
| title_short | Quantitative Phase Imaging During Cell Division Based on Dual-Channel Microscopic Interferometry System |
| title_sort | quantitative phase imaging during cell division based on dual channel microscopic interferometry system |
| topic | Quantitative phase imaging dual-channel microscopic interferometry phase shift cell division. |
| url | https://ieeexplore.ieee.org/document/8924630/ |
| work_keys_str_mv | AT shengdeliu quantitativephaseimagingduringcelldivisionbasedondualchannelmicroscopicinterferometrysystem AT dejinzheng quantitativephaseimagingduringcelldivisionbasedondualchannelmicroscopicinterferometrysystem AT jiaoshengli quantitativephaseimagingduringcelldivisionbasedondualchannelmicroscopicinterferometrysystem AT jindongtian quantitativephaseimagingduringcelldivisionbasedondualchannelmicroscopicinterferometrysystem AT liyunzhong quantitativephaseimagingduringcelldivisionbasedondualchannelmicroscopicinterferometrysystem AT xiaoxulu quantitativephaseimagingduringcelldivisionbasedondualchannelmicroscopicinterferometrysystem |