The Role of <i>Megalobrama amblycephala bcl2l13</i> Gene in Apoptosis and Autophagy
Bcl-2-like protein 13 (Bcl2l13) plays an important role in the cell apoptosis and mitochondrial autophagy of mammals. However, the role of <i>bcl2l13</i> remains unclear in fish. Therefore, in this study, the function of <i>Megalobrama amblycephala bcl2l13</i> gene in apoptos...
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MDPI AG
2025-05-01
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| author | Suzhen Wang Xuanhui Li Danyang Wu Zexia Gao Hong Liu Huanling Wang |
| author_facet | Suzhen Wang Xuanhui Li Danyang Wu Zexia Gao Hong Liu Huanling Wang |
| author_sort | Suzhen Wang |
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| description | Bcl-2-like protein 13 (Bcl2l13) plays an important role in the cell apoptosis and mitochondrial autophagy of mammals. However, the role of <i>bcl2l13</i> remains unclear in fish. Therefore, in this study, the function of <i>Megalobrama amblycephala bcl2l13</i> gene in apoptosis and autophagy was investigated. The results showed that the overexpression of <i>M. amblycephala bcl2l13</i> under hypoxic condition led to a reduction of reactive oxygen species (ROS), an increase in the expression levels of autophagy-related genes (<i>p62</i>, <i>lc3</i>, <i>pink1</i>), and a disruption of mitochondrial structure. However, deleting its transmembrane (TM) and Bcl-2 homology no (BHNo) domains decreased the P62 protein level, suggesting its essential role in autophagy. Furthermore, <i>bcl2l13</i> overexpression inhibited cell proliferation and increased apoptosis. Additional studies revealed that the permeability of the mitochondrial permeability transition pore (mPTP) increased after overexpression of <i>bcl2l13</i>, but decreased upon deletion of the TM domain. Additionally, hypoxia led to elevated Bcl2l13 and P62 levels, and caused mitochondrial damage in <i>M. amblycephala</i> liver after 48 h of treatment. In conclusion, <i>bcl2l13</i> may induce autophagy, inhibit cell proliferation and promote apoptosis, while its TM and BHNo domains play pivotal roles in these processes. |
| format | Article |
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| language | English |
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| spelling | doaj-art-4d50aef5d1284b84ba89cda88ee9d0782025-06-25T13:49:30ZengMDPI AGFishes2410-38882025-05-0110624710.3390/fishes10060247The Role of <i>Megalobrama amblycephala bcl2l13</i> Gene in Apoptosis and AutophagySuzhen Wang0Xuanhui Li1Danyang Wu2Zexia Gao3Hong Liu4Huanling Wang5College of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affairs/Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affairs/Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affairs/Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affairs/Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affairs/Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affairs/Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaBcl-2-like protein 13 (Bcl2l13) plays an important role in the cell apoptosis and mitochondrial autophagy of mammals. However, the role of <i>bcl2l13</i> remains unclear in fish. Therefore, in this study, the function of <i>Megalobrama amblycephala bcl2l13</i> gene in apoptosis and autophagy was investigated. The results showed that the overexpression of <i>M. amblycephala bcl2l13</i> under hypoxic condition led to a reduction of reactive oxygen species (ROS), an increase in the expression levels of autophagy-related genes (<i>p62</i>, <i>lc3</i>, <i>pink1</i>), and a disruption of mitochondrial structure. However, deleting its transmembrane (TM) and Bcl-2 homology no (BHNo) domains decreased the P62 protein level, suggesting its essential role in autophagy. Furthermore, <i>bcl2l13</i> overexpression inhibited cell proliferation and increased apoptosis. Additional studies revealed that the permeability of the mitochondrial permeability transition pore (mPTP) increased after overexpression of <i>bcl2l13</i>, but decreased upon deletion of the TM domain. Additionally, hypoxia led to elevated Bcl2l13 and P62 levels, and caused mitochondrial damage in <i>M. amblycephala</i> liver after 48 h of treatment. In conclusion, <i>bcl2l13</i> may induce autophagy, inhibit cell proliferation and promote apoptosis, while its TM and BHNo domains play pivotal roles in these processes.https://www.mdpi.com/2410-3888/10/6/247<i>bcl2l13</i>fishapoptosisautophagy |
| spellingShingle | Suzhen Wang Xuanhui Li Danyang Wu Zexia Gao Hong Liu Huanling Wang The Role of <i>Megalobrama amblycephala bcl2l13</i> Gene in Apoptosis and Autophagy Fishes <i>bcl2l13</i> fish apoptosis autophagy |
| title | The Role of <i>Megalobrama amblycephala bcl2l13</i> Gene in Apoptosis and Autophagy |
| title_full | The Role of <i>Megalobrama amblycephala bcl2l13</i> Gene in Apoptosis and Autophagy |
| title_fullStr | The Role of <i>Megalobrama amblycephala bcl2l13</i> Gene in Apoptosis and Autophagy |
| title_full_unstemmed | The Role of <i>Megalobrama amblycephala bcl2l13</i> Gene in Apoptosis and Autophagy |
| title_short | The Role of <i>Megalobrama amblycephala bcl2l13</i> Gene in Apoptosis and Autophagy |
| title_sort | role of i megalobrama amblycephala bcl2l13 i gene in apoptosis and autophagy |
| topic | <i>bcl2l13</i> fish apoptosis autophagy |
| url | https://www.mdpi.com/2410-3888/10/6/247 |
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