Thymus praecox subsp. polytrichus: Antimicrobial and antidiabetic activity of ethanol extracts
Thymus praecox is one of the most widespread species in the Euro-Siberian region. This study was conducted to determine the in vitro antimicrobial and anti-diabetic activity of its plant extracts prepared with 70% and 96% ethanol, with and without pretreatment with n-hexane, using the Soxhl...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Matica srpska
2025-01-01
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| Series: | Zbornik Matice Srpske za Prirodne Nauke |
| Subjects: | |
| Online Access: | https://doiserbia.nb.rs/img/doi/0352-4906/2025/0352-49062548017G.pdf |
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| Summary: | Thymus praecox is one of the most widespread species in the Euro-Siberian
region. This study was conducted to determine the in vitro antimicrobial and
anti-diabetic activity of its plant extracts prepared with 70% and 96%
ethanol, with and without pretreatment with n-hexane, using the Soxhlet
apparatus. The MICs and MBCs ranged from 0.035 to 0.150 mg/mL and from 0.075
to 0.300 mg/mL, respectively. Both 96% ethanol extracts showed the most
promising antibacterial activity, especially against Bacillus cereus,
Enterobacter cloacae and Salmonella Typhimurium. The pretreated 96%
ethanol extract also showed remarkable antimicrobial activity against
Staphylococcus aureus. Bacteria B. cereus, S. aureus and S. typhimurium were
also sensitive to the pretreated 70% ethanol extract, while 70% ethanol was
most effective against S. aureus and Escherichia coli. The MICs and MFCs
varied from 0.017 to 0.30 mg/mL and from 0.035 to 0.70 mg/mL, respectively,
with Aspergillus versicolor and Trichoderma viride being the most sensitive.
The IC50 values ranged from 0.94 to 1.40 mg/mL for α-amylase and from 76.10
to 610.2 µg/mL for α-glucosidase. The 70% ethanol extract, especially with
the hexane pretreatment, showed the highest enzyme inhibitory activity in
the α-glucosidase assay (76.10 µg/mL), even higher than the control,
acarbose/ glucobay (20.46 µg/mL). The highest α-amylase inhibitory effect
was obtained for the 96% ethanol extract. The results show that the extracts
of T. praecox subsp. polytrichus have significant antimicrobial and
antidiabetic potential and can be used for food preservation and
phytopharmacy. |
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| ISSN: | 0352-4906 2406-0828 |