Establishment and Application of Loop-Mediated Isothermal Amplification Assays for Pathogens of Rice Bakanae Disease
Rice bakanae disease (RBD), a major threat in rice-cropping nations, can reduce rice yield and quality. As it is a seed-borne disease, effective seed detection is crucial. Loop-mediated isothermal amplification (LAMP) can rapidly and specifically amplify DNA at a constant temperature with high sensi...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-06-01
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| Series: | Agriculture |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2077-0472/15/12/1319 |
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| Summary: | Rice bakanae disease (RBD), a major threat in rice-cropping nations, can reduce rice yield and quality. As it is a seed-borne disease, effective seed detection is crucial. Loop-mediated isothermal amplification (LAMP) can rapidly and specifically amplify DNA at a constant temperature with high sensitivity. This research uses LAMP to develop rapid RBD pathogen detection systems. Primers were designed targeting the <i>NRPS31</i> gene of <i>Fusarium fujikuroi</i> and conserved <i>TEF1α</i> sequences of <i>Fusarium asiaticum</i>, <i>Fusarium proliferatum</i>, and <i>Fusarium andiyazi</i>. These reactions at 60 °C for 60 min had a detection limit of 100 pg·μL<sup>−1</sup>, and LAMP proved applicable in field trials. |
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| ISSN: | 2077-0472 |