The effect of chrysin–curcumin-loaded nanofibres on the wound-healing process in male rats
Aim The aim of the present study was to produce chrysin–curcumin-loaded PCL-PEG nanofibres by an electrospinning technique and to evaluate the biological activity of the chrysin–curcumin-loaded PCL-PEG fibres for wound healing and its related genes using in vivo methods.Materials and methods The ele...
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| Format: | Article |
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Taylor & Francis Group
2019-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2019.1594855 |
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| author | Zoheyr Mohammadi Mohsen Sharif Zak Hasan Majdi Ebrahim Mostafavi Meisam Barati Hamid Lotfimehr Kambiz Ghaseminasab Hamidreza Pazoki-Toroudi Thomas J. Webster Abolfazl Akbarzadeh |
| author_facet | Zoheyr Mohammadi Mohsen Sharif Zak Hasan Majdi Ebrahim Mostafavi Meisam Barati Hamid Lotfimehr Kambiz Ghaseminasab Hamidreza Pazoki-Toroudi Thomas J. Webster Abolfazl Akbarzadeh |
| author_sort | Zoheyr Mohammadi |
| collection | DOAJ |
| description | Aim The aim of the present study was to produce chrysin–curcumin-loaded PCL-PEG nanofibres by an electrospinning technique and to evaluate the biological activity of the chrysin–curcumin-loaded PCL-PEG fibres for wound healing and its related genes using in vivo methods.Materials and methods The electrospinning method was carried out for the preparation of the chrysin, curcumin and chrysin–curcumin-loaded PCL-PEG nanofibres with different concentrations. FTIR and SEM were performed to characterize the chemical structures and morphology of the nanofibres. In vitro drug release, as well as in vivo wound-healing studies were investigated in male rats. The expressions of genes related to the wound-healing process were also evaluated by real-time PCR.Results Our study showed that the chrysin–curcumin-loaded nanofibres have anti-inflammatory properties in several stages of the wound-healing process by affecting the IL-6, MMP-2, TIMP-1, TIMP-2 and iNOS gene expression. Our results demonstrated that the effect of the chrysin-loaded nanofibre, the curcumin-loaded nanofibre and the chrysin–curcumin-loaded nanofibre in the wound-healing process is dose dependent and in accordance with the obtained results in that it might affect the inflammation phase more than the other stages of the wound-healing process.Conclusion We have introduced chrysin–curcumin-loaded PCL-PEG nanofibres as a novel compound for shortening the duration of the wound-healing process. |
| format | Article |
| id | doaj-art-4aff1f2dae6f41e197d7e68f4c6d28f0 |
| institution | Matheson Library |
| issn | 2169-1401 2169-141X |
| language | English |
| publishDate | 2019-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj-art-4aff1f2dae6f41e197d7e68f4c6d28f02025-07-21T21:25:21ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2019-12-014711642165210.1080/21691401.2019.1594855The effect of chrysin–curcumin-loaded nanofibres on the wound-healing process in male ratsZoheyr Mohammadi0Mohsen Sharif Zak1Hasan Majdi2Ebrahim Mostafavi3Meisam Barati4Hamid Lotfimehr5Kambiz Ghaseminasab6Hamidreza Pazoki-Toroudi7Thomas J. Webster8Abolfazl Akbarzadeh9Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Clinical Biochemistry, Faculty of Medical Sciences, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Medical Nanotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Chemical Engineering, Northeastern University, Boston, MA, USASchool of Nutrition and Food Technology, Cellular and Molecular Nutrition Department, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Applied Cell Sciences, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Biochemistry, School of Medicine, Iran University of Medical Sciences, Tehran, IranPhysiology Research Center and Department of Physiology, Iran University of Medical Sciences, Tehran, IranDepartment of Chemical Engineering, Northeastern University, Boston, MA, USATuberculosis and Lung Disease Research Center of Tabriz, Tabriz University of Medical Sciences, Tabriz, IranAim The aim of the present study was to produce chrysin–curcumin-loaded PCL-PEG nanofibres by an electrospinning technique and to evaluate the biological activity of the chrysin–curcumin-loaded PCL-PEG fibres for wound healing and its related genes using in vivo methods.Materials and methods The electrospinning method was carried out for the preparation of the chrysin, curcumin and chrysin–curcumin-loaded PCL-PEG nanofibres with different concentrations. FTIR and SEM were performed to characterize the chemical structures and morphology of the nanofibres. In vitro drug release, as well as in vivo wound-healing studies were investigated in male rats. The expressions of genes related to the wound-healing process were also evaluated by real-time PCR.Results Our study showed that the chrysin–curcumin-loaded nanofibres have anti-inflammatory properties in several stages of the wound-healing process by affecting the IL-6, MMP-2, TIMP-1, TIMP-2 and iNOS gene expression. Our results demonstrated that the effect of the chrysin-loaded nanofibre, the curcumin-loaded nanofibre and the chrysin–curcumin-loaded nanofibre in the wound-healing process is dose dependent and in accordance with the obtained results in that it might affect the inflammation phase more than the other stages of the wound-healing process.Conclusion We have introduced chrysin–curcumin-loaded PCL-PEG nanofibres as a novel compound for shortening the duration of the wound-healing process.https://www.tandfonline.com/doi/10.1080/21691401.2019.1594855Wound healingchrysincurcuminMMPsTIMPs |
| spellingShingle | Zoheyr Mohammadi Mohsen Sharif Zak Hasan Majdi Ebrahim Mostafavi Meisam Barati Hamid Lotfimehr Kambiz Ghaseminasab Hamidreza Pazoki-Toroudi Thomas J. Webster Abolfazl Akbarzadeh The effect of chrysin–curcumin-loaded nanofibres on the wound-healing process in male rats Artificial Cells, Nanomedicine, and Biotechnology Wound healing chrysin curcumin MMPs TIMPs |
| title | The effect of chrysin–curcumin-loaded nanofibres on the wound-healing process in male rats |
| title_full | The effect of chrysin–curcumin-loaded nanofibres on the wound-healing process in male rats |
| title_fullStr | The effect of chrysin–curcumin-loaded nanofibres on the wound-healing process in male rats |
| title_full_unstemmed | The effect of chrysin–curcumin-loaded nanofibres on the wound-healing process in male rats |
| title_short | The effect of chrysin–curcumin-loaded nanofibres on the wound-healing process in male rats |
| title_sort | effect of chrysin curcumin loaded nanofibres on the wound healing process in male rats |
| topic | Wound healing chrysin curcumin MMPs TIMPs |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2019.1594855 |
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