Specificities of multi-primer polymerase chain reaction optimization for the detection of infectious pneumonia agents in human
Objectives. The objectives of this work are the development of a multi-primer system based on the polymerase chain reaction (PCR) aimed at the simultaneous detection of six bacterial pathogens that cause human pneumonia and the determination of the parameters important for the optimization of this m...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
MIREA - Russian Technological University
2021-07-01
|
| Series: | Тонкие химические технологии |
| Subjects: | |
| Online Access: | https://www.finechem-mirea.ru/jour/article/view/1710 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1839581280114573312 |
|---|---|
| author | E. S. Klochikhina V. E. Shershov V. E. Kuznetsova S. A. Lapa A. V. Chudinov |
| author_facet | E. S. Klochikhina V. E. Shershov V. E. Kuznetsova S. A. Lapa A. V. Chudinov |
| author_sort | E. S. Klochikhina |
| collection | DOAJ |
| description | Objectives. The objectives of this work are the development of a multi-primer system based on the polymerase chain reaction (PCR) aimed at the simultaneous detection of six bacterial pathogens that cause human pneumonia and the determination of the parameters important for the optimization of this multi-primer system, including solid-phase PCR systems (biological microarrays).Methods. To determine the optimal parameters of the system, PCR methods were used in monoplex and multiplex formats.Results. Primers for Staphylococcus aureus, Pseudomonas aeruginosa, Haemophilus influenza, Legionella pneumophila, Klebsiella pneumoniae, and Streptococcus pneumoniae detection were designed, and the PCR cycling conditions were optimized. The patterns of primer design for solidphase PCR were revealed.Conclusions. The developed prototype of a system specifically identifies six clinically significant bacterial pathogens. It could be expanded for the analysis of viral and fungal pathogens and used in clinical diagnostics. A prototype of a system for pathogenic agent detection in the immobilized phase (biological microarray) was created. |
| format | Article |
| id | doaj-art-49fba6f0f3034ce9af72acad3ae1005f |
| institution | Matheson Library |
| issn | 2410-6593 2686-7575 |
| language | Russian |
| publishDate | 2021-07-01 |
| publisher | MIREA - Russian Technological University |
| record_format | Article |
| series | Тонкие химические технологии |
| spelling | doaj-art-49fba6f0f3034ce9af72acad3ae1005f2025-08-04T10:19:39ZrusMIREA - Russian Technological UniversityТонкие химические технологии2410-65932686-75752021-07-0116322523110.32362/2410-6593-2021-16-3-225-2311638Specificities of multi-primer polymerase chain reaction optimization for the detection of infectious pneumonia agents in humanE. S. Klochikhina0V. E. Shershov1V. E. Kuznetsova2S. A. Lapa3A. V. Chudinov4Engelhardt Institute of Molecular Biology, Russian Academy of SciencesEngelhardt Institute of Molecular Biology, Russian Academy of SciencesEngelhardt Institute of Molecular Biology, Russian Academy of SciencesEngelhardt Institute of Molecular Biology, Russian Academy of SciencesEngelhardt Institute of Molecular Biology, Russian Academy of SciencesObjectives. The objectives of this work are the development of a multi-primer system based on the polymerase chain reaction (PCR) aimed at the simultaneous detection of six bacterial pathogens that cause human pneumonia and the determination of the parameters important for the optimization of this multi-primer system, including solid-phase PCR systems (biological microarrays).Methods. To determine the optimal parameters of the system, PCR methods were used in monoplex and multiplex formats.Results. Primers for Staphylococcus aureus, Pseudomonas aeruginosa, Haemophilus influenza, Legionella pneumophila, Klebsiella pneumoniae, and Streptococcus pneumoniae detection were designed, and the PCR cycling conditions were optimized. The patterns of primer design for solidphase PCR were revealed.Conclusions. The developed prototype of a system specifically identifies six clinically significant bacterial pathogens. It could be expanded for the analysis of viral and fungal pathogens and used in clinical diagnostics. A prototype of a system for pathogenic agent detection in the immobilized phase (biological microarray) was created.https://www.finechem-mirea.ru/jour/article/view/1710infectious pneumoniamultiplex pcrbiochipscovid-19 |
| spellingShingle | E. S. Klochikhina V. E. Shershov V. E. Kuznetsova S. A. Lapa A. V. Chudinov Specificities of multi-primer polymerase chain reaction optimization for the detection of infectious pneumonia agents in human Тонкие химические технологии infectious pneumonia multiplex pcr biochips covid-19 |
| title | Specificities of multi-primer polymerase chain reaction optimization for the detection of infectious pneumonia agents in human |
| title_full | Specificities of multi-primer polymerase chain reaction optimization for the detection of infectious pneumonia agents in human |
| title_fullStr | Specificities of multi-primer polymerase chain reaction optimization for the detection of infectious pneumonia agents in human |
| title_full_unstemmed | Specificities of multi-primer polymerase chain reaction optimization for the detection of infectious pneumonia agents in human |
| title_short | Specificities of multi-primer polymerase chain reaction optimization for the detection of infectious pneumonia agents in human |
| title_sort | specificities of multi primer polymerase chain reaction optimization for the detection of infectious pneumonia agents in human |
| topic | infectious pneumonia multiplex pcr biochips covid-19 |
| url | https://www.finechem-mirea.ru/jour/article/view/1710 |
| work_keys_str_mv | AT esklochikhina specificitiesofmultiprimerpolymerasechainreactionoptimizationforthedetectionofinfectiouspneumoniaagentsinhuman AT veshershov specificitiesofmultiprimerpolymerasechainreactionoptimizationforthedetectionofinfectiouspneumoniaagentsinhuman AT vekuznetsova specificitiesofmultiprimerpolymerasechainreactionoptimizationforthedetectionofinfectiouspneumoniaagentsinhuman AT salapa specificitiesofmultiprimerpolymerasechainreactionoptimizationforthedetectionofinfectiouspneumoniaagentsinhuman AT avchudinov specificitiesofmultiprimerpolymerasechainreactionoptimizationforthedetectionofinfectiouspneumoniaagentsinhuman |