MGV-seq: a sensitive and culture-independent method for detecting microbial genetic variation

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MGV-seq: a sensitive and culture-independent method for detecting microbial genetic variation

BackgroundPrecise detection of microbial genetic variation (MGV) at the strain level is essential for reliable disease diagnosis, pathogen surveillance, and reproducible research. Current methods, however, are constrained by limited sensitivity, specificity, and dependence on culturing. To address t...

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Main Authors:	Lun Li, Weiyu Kong, Jing Sun, Yongzhong Jiang, Tiantian Li, Zhihui Xia, Junfei Zhou, Zhiwei Fang, Lihong Chen, Shun Feng, Huiyin Song, Huafeng Xiao, Baolong Zhang, Bin Fang, Hai Peng, Lifen Gao
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2025-06-01
Series:	Frontiers in Microbiology
Subjects:	microbial genetic variation multiple dispersed nucleotide polymorphism markers multiplex polymerase chain reaction MGV-Seq strain purification identification authentication
Online Access:	https://www.frontiersin.org/articles/10.3389/fmicb.2025.1603255/full
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