Morphological and chemical characterization of magnesium phosphate and calcium phosphate bions
Aim. To compare morphological properties, mineral, and organic pattern of magnesium phosphate bions (MPB) and calcium phosphate bions (CPB) for the assessment of MPB as a control group for the evaluation of СРВ-specific endothelial toxicity.Materials and Methods. Both MPB and CPB were artificially s...
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| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | Russian |
| Published: |
Kemerovo State Medical University
2019-06-01
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| Series: | Фундаментальная и клиническая медицина |
| Subjects: | |
| Online Access: | https://fcm.kemsmu.ru/jour/article/view/137 |
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| Summary: | Aim. To compare morphological properties, mineral, and organic pattern of magnesium phosphate bions (MPB) and calcium phosphate bions (CPB) for the assessment of MPB as a control group for the evaluation of СРВ-specific endothelial toxicity.Materials and Methods. Both MPB and CPB were artificially synthesized employing blood-mimetic medium supersaturated of magnesium and phosphorus salts, respectively. Morphology of MPB and CPB was investigated by electron and atomic force microscopy, elemental analysis was performed utilizing energy-dispersive X-ray spectroscopy, atomic emission spectroscopy, and CHN-SO analysis, functional groups were examined using Fourier-transform infrared spectroscopy and Raman spectroscopy, while chemical formula was identified by X-ray powder diffraction analysis. Protein profile of MPB and СРВ was screened employing polyacrylamide gel electrophoresis with the following silver staining.Results. Both MPB and CPB represented spherical spongeous particles of 80-200 nm diameter and mean diameter of around 120 nm, tending to form clusters of several particles. Both MPB and CPB contained carbon, oxygen, nitrogen, hydrogen, and phosphorus. However, MPB also contained magnesium but had minimum calcium content while СРВ had a significant amount of calcium but were devoid of magnesium. Both MPB and CPB contained phosphate, carbonate, and hydroxyl functional groups but MPB consisted of magnesium phosphate hydrate and huntite while CPB were comprised of hydroxyapatite and carbonate-hydroxyapatite. Protein composition of MPB and СРВ was similar.Conclusion. MPB are similar to CPB excepting the chemical formula; hence, MPB can be considered as an appropriate control group to evaluate specificity of СРВ-related endothelial toxicity. |
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| ISSN: | 2500-0764 2542-0941 |