小反刍兽疫LAMP反应结果可视化检测方法比较研究
环介导等温扩增(loop-mediated isothermal amplification,LAMP)技术是一种可应用于各种病原体检测、基因分型等领域的等温扩增技术。该技术所需设备简单且易于操作,因此具有较大的发展潜力。根据目前已经存在的判定LAMP结果的可视化方法,以可能对野生小反刍动物造成威胁的小反刍兽疫病毒(Peste des petits ruminants virus,PPRV)cDNA为检测对象,选择合适的引物组,比较评估均可在日光或紫外光下区分阴阳性结果的SYBR Green I、SYBR Safe Stain和羟基萘酚蓝(hydroxy naphthol blue,HNB)3...
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Main Authors: | , , , |
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Format: | Article |
Language: | Chinese |
Published: |
Editorial Department of Chinese Journal of Wildlife
2024-08-01
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Series: | 野生动物学报 |
Subjects: | |
Online Access: | http://ysdw.nefu.edu.cn/thesisDetails#10.12375/ysdwxb.20240324 |
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