<i>Eucommia ulmoides</i> leaves and <i>Gardenia</i> extracts promote white adipocyte browning by inhibiting autophagy

<i>Eucommia ulmoides</i> leaves and <i>Gardenia</i> extracts promote white adipocyte browning by inhibiting autophagy

Objective To investigate whether extracts of <i>Eucommia ulmoides</i> leaves and <i>Gardenia</i> have the effects on promoting white adipose browning， and to explore their possible mechanisms. Methods The effects of <i>Eucommia ulmoides</i> leaves and <i>Gar...

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Main Author: XIONG Shaofeng, HU Zhi, FANG Yang, ZHAO Le, ZHANG Jie, GUO Muying, WEI Li, WANG Kun
Format: Article
Language:Chinese
Published: Editorial Office of Journal of New Medicine 2025-06-01
Series:Xin yixue
Subjects:
<i>eucommia ulmoides</i> leaf extract|<i>gardenia</i> extract|autophagy|browning of white adipocyte|obesity|lipid droplet accumulation
Online Access:https://www.xinyixue.cn/fileup/0253-9802/PDF/1751417424229-1188785009.pdf
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Summary:Objective To investigate whether extracts of <i>Eucommia ulmoides</i> leaves and <i>Gardenia</i> have the effects on promoting white adipose browning， and to explore their possible mechanisms. Methods The effects of <i>Eucommia ulmoides</i> leaves and <i>Gardenia</i> extracts on mature 3T3-L1 cells were detected using CCK8 assay， Western blotting， qRT-PCR， Oil red O staining， BODIPY staining， and mitochondrial fluorescent dye. Subsequently， autophagy inhibitors （rapamycin） were used to explore their possible mechanisms of action. Results <i>Eucommia ulmoides</i> leaves and <i>Gardenia</i> extracts could upregulate browning-related proteins and mRNAs （PRDM16， PGC-1α and UCP-1） （all <i>P &lt;</i> 0.05）， inhibited autophagy-related proteins （Beclin-1 and p62） and mRNAs （LC3， Beclin-1 and p62） and the expression of autophagosomes （all <i>P &lt;</i> 0.05）， inhibited lipid droplet accumulation， and increased mitochondrial abundance. The use of autophagy activator （rapamycin） restored the autophagy effect of <i>Eucommia ulmoides</i> leaf and Gardenia extracts on 3T3-L1 cells （all <i>P &lt;</i> 0.05）， and it was found that the browning effect of <i>Eucommia ulmoides</i> leaf and <i>Gardenia</i> extracts on 3T3-L1 cells was also reversed by autophagy activator （all <i>P &lt;</i> 0.05）. Conclusion Extracts of <i>Eucommia ulmoides</i> leaves and <i>Gardenia</i> have the effects upon promoting the browning of white adipocytes by inhibiting autophagy， demonstrating potential clinical applications in the clinical prevention and treatment of obesity and related metabolic diseases.
ISSN:0253-9802

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