Molecular evidence of Enterocytozoon bieneusi in arid urban landscapes of shiraz cockroaches (Blattodea), Southwest Iran: Implications for urban public health surveillance
Microsporidia, particularly Enterocytozoon bieneusi and Encephalitozoon intestinalis, are emerging fungal pathogens of global concern, causing severe gastrointestinal and systemic infections in immunocompromised individuals. Cockroaches (Blattodea), as synanthropic pests, are potential mechanical ve...
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| Format: | Article |
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Elsevier
2025-08-01
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| Series: | Parasite Epidemiology and Control |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S240567312500039X |
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| author | Mohsen Kalantari Kourosh Azizi Negin Kiani Junaghani Mozaffar Vahedi Iraj Mohammadpour Qasem Asgari Amin Hosseinpour Mehdi Miri |
| author_facet | Mohsen Kalantari Kourosh Azizi Negin Kiani Junaghani Mozaffar Vahedi Iraj Mohammadpour Qasem Asgari Amin Hosseinpour Mehdi Miri |
| author_sort | Mohsen Kalantari |
| collection | DOAJ |
| description | Microsporidia, particularly Enterocytozoon bieneusi and Encephalitozoon intestinalis, are emerging fungal pathogens of global concern, causing severe gastrointestinal and systemic infections in immunocompromised individuals. Cockroaches (Blattodea), as synanthropic pests, are potential mechanical vectors for disseminating these pathogens in urban environments. Despite their clinical significance, data on the role of cockroaches in transmitting microsporidia in arid regions remain scarce. This study assessed the contamination of cockroaches in Shiraz, Iran, with E. bieneusi and E. intestinalis to evaluate their public health risks. From January to June 2023, 378 cockroaches were collected from high-risk areas, including Hospitals and adjacent public areas. Specimens were morphologically identified and screened via microscopy. Molecular detection of E. bieneusi and E. intestinalis was performed using PCR targeting the 18sRNA region. DNA was extracted from pooled samples (10 cockroaches/pool) and amplified under standardized thermocycling conditions. All cockroaches were identified as Periplaneta americana (77.5 %) and Blattella germanica (22.5 %). In molecular assay, three positive case of E. bieneusi was detected, However, no E. intestinalis DNA was identified via PCR. This study reports the first molecular detection of E. bieneusi in Shiraz cockroaches. In continue, it records no evidence of E. intestinalis, which suggesting climatic or environmental barriers to limit this microsporidian persistence in this arid region. These findings highlight the need for integrated vector management and advanced molecular surveillance to elucidate the epidemiology of understudied pathogens like microsporidia in rapidly urbanizing regions. |
| format | Article |
| id | doaj-art-43f6b08dfdd942388ea83c8f14d7d823 |
| institution | Matheson Library |
| issn | 2405-6731 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Parasite Epidemiology and Control |
| spelling | doaj-art-43f6b08dfdd942388ea83c8f14d7d8232025-07-12T04:46:28ZengElsevierParasite Epidemiology and Control2405-67312025-08-0130e00446Molecular evidence of Enterocytozoon bieneusi in arid urban landscapes of shiraz cockroaches (Blattodea), Southwest Iran: Implications for urban public health surveillanceMohsen Kalantari0Kourosh Azizi1Negin Kiani Junaghani2Mozaffar Vahedi3Iraj Mohammadpour4Qasem Asgari5Amin Hosseinpour6Mehdi Miri7Research Center for Health Sciences, Institute of Health, Department of Biology and Control of Disease Vectors, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran; Corresponding author at: Research Center for Health Sciences, Institute of Health, Shiraz University of Medical Sciences, Shiraz, Iran.Research Center for Health Sciences, Institute of Health, Department of Biology and Control of Disease Vectors, School of Health, Shiraz University of Medical Sciences, Shiraz, IranStudent Research Committee, Department of Biology and Control of Disease Vectors, School of Health, Shiraz University of Medical Sciences, Shiraz, IranResearch Center for Health Sciences, Institute of Health, Department of Biology and Control of Disease Vectors, School of Health, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Medical Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Medical Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranResearch Center for Health Sciences, Institute of Health, Department of Biology and Control of Disease Vectors, School of Health, Shiraz University of Medical Sciences, Shiraz, IranStudent Research Committee, Department of Biology and Control of Disease Vectors, School of Health, Shiraz University of Medical Sciences, Shiraz, IranMicrosporidia, particularly Enterocytozoon bieneusi and Encephalitozoon intestinalis, are emerging fungal pathogens of global concern, causing severe gastrointestinal and systemic infections in immunocompromised individuals. Cockroaches (Blattodea), as synanthropic pests, are potential mechanical vectors for disseminating these pathogens in urban environments. Despite their clinical significance, data on the role of cockroaches in transmitting microsporidia in arid regions remain scarce. This study assessed the contamination of cockroaches in Shiraz, Iran, with E. bieneusi and E. intestinalis to evaluate their public health risks. From January to June 2023, 378 cockroaches were collected from high-risk areas, including Hospitals and adjacent public areas. Specimens were morphologically identified and screened via microscopy. Molecular detection of E. bieneusi and E. intestinalis was performed using PCR targeting the 18sRNA region. DNA was extracted from pooled samples (10 cockroaches/pool) and amplified under standardized thermocycling conditions. All cockroaches were identified as Periplaneta americana (77.5 %) and Blattella germanica (22.5 %). In molecular assay, three positive case of E. bieneusi was detected, However, no E. intestinalis DNA was identified via PCR. This study reports the first molecular detection of E. bieneusi in Shiraz cockroaches. In continue, it records no evidence of E. intestinalis, which suggesting climatic or environmental barriers to limit this microsporidian persistence in this arid region. These findings highlight the need for integrated vector management and advanced molecular surveillance to elucidate the epidemiology of understudied pathogens like microsporidia in rapidly urbanizing regions.http://www.sciencedirect.com/science/article/pii/S240567312500039XEnterocytozoon bieneusiEncephalitozoon intestinalisMicrosporidiaBlattodeaPCRIran |
| spellingShingle | Mohsen Kalantari Kourosh Azizi Negin Kiani Junaghani Mozaffar Vahedi Iraj Mohammadpour Qasem Asgari Amin Hosseinpour Mehdi Miri Molecular evidence of Enterocytozoon bieneusi in arid urban landscapes of shiraz cockroaches (Blattodea), Southwest Iran: Implications for urban public health surveillance Parasite Epidemiology and Control Enterocytozoon bieneusi Encephalitozoon intestinalis Microsporidia Blattodea PCR Iran |
| title | Molecular evidence of Enterocytozoon bieneusi in arid urban landscapes of shiraz cockroaches (Blattodea), Southwest Iran: Implications for urban public health surveillance |
| title_full | Molecular evidence of Enterocytozoon bieneusi in arid urban landscapes of shiraz cockroaches (Blattodea), Southwest Iran: Implications for urban public health surveillance |
| title_fullStr | Molecular evidence of Enterocytozoon bieneusi in arid urban landscapes of shiraz cockroaches (Blattodea), Southwest Iran: Implications for urban public health surveillance |
| title_full_unstemmed | Molecular evidence of Enterocytozoon bieneusi in arid urban landscapes of shiraz cockroaches (Blattodea), Southwest Iran: Implications for urban public health surveillance |
| title_short | Molecular evidence of Enterocytozoon bieneusi in arid urban landscapes of shiraz cockroaches (Blattodea), Southwest Iran: Implications for urban public health surveillance |
| title_sort | molecular evidence of enterocytozoon bieneusi in arid urban landscapes of shiraz cockroaches blattodea southwest iran implications for urban public health surveillance |
| topic | Enterocytozoon bieneusi Encephalitozoon intestinalis Microsporidia Blattodea PCR Iran |
| url | http://www.sciencedirect.com/science/article/pii/S240567312500039X |
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