ELISPOT assay of the SARS-CoV-2 specific T cells immune response
The COVID-19 pandemic has stimulated interest in the development of biotechnology, as well as in the search for new solutions in the diagnostics of immune processes. The response of immunoglobulins A, M and G had a significant role in the assessment of virus-specific immune responses. Later, it was...
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| Format: | Article |
| Language: | Russian |
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Scientific Сentre for Family Health and Human Reproduction Problems
2022-12-01
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| Series: | Acta Biomedica Scientifica |
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| Online Access: | https://www.actabiomedica.ru/jour/article/view/3829 |
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| author | V. V. Gerasimova S. V. Kolesnik D. A. Kudlay A. S. Golderova |
| author_facet | V. V. Gerasimova S. V. Kolesnik D. A. Kudlay A. S. Golderova |
| author_sort | V. V. Gerasimova |
| collection | DOAJ |
| description | The COVID-19 pandemic has stimulated interest in the development of biotechnology, as well as in the search for new solutions in the diagnostics of immune processes. The response of immunoglobulins A, M and G had a significant role in the assessment of virus-specific immune responses. Later, it was understood that for a comprehensive assessment of adaptive immunity processes, it is reasonable to study its cellular component. One of the most affordable methods for assessing T cell immunity, which has proven itself in the diagnosis of other infectious diseases, such as latent tuberculosis infection, is IGRA ELISPOT.The aim of the study. To determine SARS-СoV-2 specific immune response of T lymphocytes in vitro in the peripheral blood of volunteers from various groups using IGRA ELISPOT method. We evaluated the applicability of the method to assess T cell immune response to infection and vaccination. In addition, we determined the duration of the maintenance period of the SARS-CoV-2 specific T cells immune response induced by vaccination.Materials and methods. The study was carried out on venous blood samples of volunteers from three groups: 1) hospital patients with COVID-19; 2) COVID-19 convalescents; 3) vaccinated against COVID-19. The T cell immune response was assessed using the TigraTest® SARS-CoV-2 test system, which determines in vitro the number of T cells secreting interferon-gamma in response to stimulation with SARS-СoV-2 peptides in two antigens panels: 1) peptides of the spike protein (S); 2) peptides of N, M, Orf3a and Orf7a proteins.Conclusion. The IGRA ELISPOT assay is a specific and sensitive tool in the assessment of T cell immunity to the SARS-CoV-2 virus. The method makes it possible to assess SARS-CoV-2 specific T cell responses induced both by natural encounter with the pathogen and by vaccination. It is advisable to use the method in routine practice for comprehensive assessment of immunity to SARS-CoV-2. |
| format | Article |
| id | doaj-art-43d9e091ebb04a6391b67bab0d4fe0c2 |
| institution | Matheson Library |
| issn | 2541-9420 2587-9596 |
| language | Russian |
| publishDate | 2022-12-01 |
| publisher | Scientific Сentre for Family Health and Human Reproduction Problems |
| record_format | Article |
| series | Acta Biomedica Scientifica |
| spelling | doaj-art-43d9e091ebb04a6391b67bab0d4fe0c22025-07-28T14:03:29ZrusScientific Сentre for Family Health and Human Reproduction ProblemsActa Biomedica Scientifica2541-94202587-95962022-12-0175-29610210.29413/ABS.2022-7.5-2.102440ELISPOT assay of the SARS-CoV-2 specific T cells immune responseV. V. Gerasimova0S. V. Kolesnik1D. A. Kudlay2A. S. Golderova3North-Eastern Federal University named after M.K. AmmosovNational Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. KulakovThe First Sechenov Moscow State Medical University (Sechenov University); National Research Center – Institute of Immunology, Federal Medical-Biological Agency of RussiaNorth-Eastern Federal University named after M.K. AmmosovThe COVID-19 pandemic has stimulated interest in the development of biotechnology, as well as in the search for new solutions in the diagnostics of immune processes. The response of immunoglobulins A, M and G had a significant role in the assessment of virus-specific immune responses. Later, it was understood that for a comprehensive assessment of adaptive immunity processes, it is reasonable to study its cellular component. One of the most affordable methods for assessing T cell immunity, which has proven itself in the diagnosis of other infectious diseases, such as latent tuberculosis infection, is IGRA ELISPOT.The aim of the study. To determine SARS-СoV-2 specific immune response of T lymphocytes in vitro in the peripheral blood of volunteers from various groups using IGRA ELISPOT method. We evaluated the applicability of the method to assess T cell immune response to infection and vaccination. In addition, we determined the duration of the maintenance period of the SARS-CoV-2 specific T cells immune response induced by vaccination.Materials and methods. The study was carried out on venous blood samples of volunteers from three groups: 1) hospital patients with COVID-19; 2) COVID-19 convalescents; 3) vaccinated against COVID-19. The T cell immune response was assessed using the TigraTest® SARS-CoV-2 test system, which determines in vitro the number of T cells secreting interferon-gamma in response to stimulation with SARS-СoV-2 peptides in two antigens panels: 1) peptides of the spike protein (S); 2) peptides of N, M, Orf3a and Orf7a proteins.Conclusion. The IGRA ELISPOT assay is a specific and sensitive tool in the assessment of T cell immunity to the SARS-CoV-2 virus. The method makes it possible to assess SARS-CoV-2 specific T cell responses induced both by natural encounter with the pathogen and by vaccination. It is advisable to use the method in routine practice for comprehensive assessment of immunity to SARS-CoV-2.https://www.actabiomedica.ru/jour/article/view/3829elispotsars-cov-2covid-19t lymphocytest cellsimmune responsetigratest®cellular immunityvaccination |
| spellingShingle | V. V. Gerasimova S. V. Kolesnik D. A. Kudlay A. S. Golderova ELISPOT assay of the SARS-CoV-2 specific T cells immune response Acta Biomedica Scientifica elispot sars-cov-2 covid-19 t lymphocytes t cells immune response tigratest® cellular immunity vaccination |
| title | ELISPOT assay of the SARS-CoV-2 specific T cells immune response |
| title_full | ELISPOT assay of the SARS-CoV-2 specific T cells immune response |
| title_fullStr | ELISPOT assay of the SARS-CoV-2 specific T cells immune response |
| title_full_unstemmed | ELISPOT assay of the SARS-CoV-2 specific T cells immune response |
| title_short | ELISPOT assay of the SARS-CoV-2 specific T cells immune response |
| title_sort | elispot assay of the sars cov 2 specific t cells immune response |
| topic | elispot sars-cov-2 covid-19 t lymphocytes t cells immune response tigratest® cellular immunity vaccination |
| url | https://www.actabiomedica.ru/jour/article/view/3829 |
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