Differentiation between <I>Yersinia pestis</I> Strains of Altaic-Hissar Group of Non-Main Subspecies by Means of PCR
On the basis of comparison between the genome-wide sequences of Altaic and Hissar strains of non-main subspecies and strains from Talas high-mountain plague focus, identified have been two novel DNA-targets, the usage of which provides for easy rapid subdivision of these closely-related strains of n...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | Russian |
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Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2015-03-01
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| Series: | Проблемы особо опасных инфекций |
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| Online Access: | https://journal.microbe.ru/jour/article/view/210 |
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| author | K. A. Nikiforov G. N. Odinokov L. A. Novichkova G. A. Eroshenko |
| author_facet | K. A. Nikiforov G. N. Odinokov L. A. Novichkova G. A. Eroshenko |
| author_sort | K. A. Nikiforov |
| collection | DOAJ |
| description | On the basis of comparison between the genome-wide sequences of Altaic and Hissar strains of non-main subspecies and strains from Talas high-mountain plague focus, identified have been two novel DNA-targets, the usage of which provides for easy rapid subdivision of these closely-related strains of non-main subspecies that fall into separate phylogenetic brunches of plague agent evolution, applying PCR assay. One of the targets is allocated to intergenic region between YPO3333 and YPO3332 genes. In strains of altaica ssp. it contains 122 bps deletion. The other target is YPO2412 gene region which contains 72 bps deletion in Talas strains. Special primers for DNA-targets have been designed. Established have also been test specifications. Efficacy of the method for differentiation between the strains of Altaic-Hissar group of non-main subspecies is validated on 97 Y. pestis strains of various ssp. |
| format | Article |
| id | doaj-art-4118045ad4ed45c7a14be26f306532e5 |
| institution | Matheson Library |
| issn | 0370-1069 2658-719X |
| language | Russian |
| publishDate | 2015-03-01 |
| publisher | Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” |
| record_format | Article |
| series | Проблемы особо опасных инфекций |
| spelling | doaj-art-4118045ad4ed45c7a14be26f306532e52025-07-07T08:02:38ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2015-03-0101717410.21055/0370-1069-2015-1-71-74210Differentiation between <I>Yersinia pestis</I> Strains of Altaic-Hissar Group of Non-Main Subspecies by Means of PCRK. A. Nikiforov0G. N. Odinokov1L. A. Novichkova2G. A. Eroshenko3Russian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”On the basis of comparison between the genome-wide sequences of Altaic and Hissar strains of non-main subspecies and strains from Talas high-mountain plague focus, identified have been two novel DNA-targets, the usage of which provides for easy rapid subdivision of these closely-related strains of non-main subspecies that fall into separate phylogenetic brunches of plague agent evolution, applying PCR assay. One of the targets is allocated to intergenic region between YPO3333 and YPO3332 genes. In strains of altaica ssp. it contains 122 bps deletion. The other target is YPO2412 gene region which contains 72 bps deletion in Talas strains. Special primers for DNA-targets have been designed. Established have also been test specifications. Efficacy of the method for differentiation between the strains of Altaic-Hissar group of non-main subspecies is validated on 97 Y. pestis strains of various ssp.https://journal.microbe.ru/jour/article/view/210возбудитель чумынеосновные подвидыдифференциацияпцрднк-мишениplague agentnon-main subspeciesdifferentiationpcrdna-targets |
| spellingShingle | K. A. Nikiforov G. N. Odinokov L. A. Novichkova G. A. Eroshenko Differentiation between <I>Yersinia pestis</I> Strains of Altaic-Hissar Group of Non-Main Subspecies by Means of PCR Проблемы особо опасных инфекций возбудитель чумы неосновные подвиды дифференциация пцр днк-мишени plague agent non-main subspecies differentiation pcr dna-targets |
| title | Differentiation between <I>Yersinia pestis</I> Strains of Altaic-Hissar Group of Non-Main Subspecies by Means of PCR |
| title_full | Differentiation between <I>Yersinia pestis</I> Strains of Altaic-Hissar Group of Non-Main Subspecies by Means of PCR |
| title_fullStr | Differentiation between <I>Yersinia pestis</I> Strains of Altaic-Hissar Group of Non-Main Subspecies by Means of PCR |
| title_full_unstemmed | Differentiation between <I>Yersinia pestis</I> Strains of Altaic-Hissar Group of Non-Main Subspecies by Means of PCR |
| title_short | Differentiation between <I>Yersinia pestis</I> Strains of Altaic-Hissar Group of Non-Main Subspecies by Means of PCR |
| title_sort | differentiation between i yersinia pestis i strains of altaic hissar group of non main subspecies by means of pcr |
| topic | возбудитель чумы неосновные подвиды дифференциация пцр днк-мишени plague agent non-main subspecies differentiation pcr dna-targets |
| url | https://journal.microbe.ru/jour/article/view/210 |
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