Effects of human umbilical cord-derived mesenchymal stem cell therapy for cavernous nerve injury-induced erectile dysfunction in the rat model
Stem cell treatment may enhance erectile dysfunction (ED) in individuals with cavernous nerve injury (CNI). Nevertheless, no investigations have directly ascertained the implications of varying amounts of human umbilical cord-derived mesenchymal stem cells (HUC-MSCs) on ED. We compare the efficacy o...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2025-07-01
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| Series: | Asian Journal of Andrology |
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| Online Access: | https://journals.lww.com/10.4103/aja2024115 |
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| author | Wei Wang Ying Liu Zi-Hao Zhou Kun Pang Jing-Kai Wang Peng-Fei Huan Jing-Ru Lu Tao Zhu Zuo-Bin Zhu Cong-Hui Han |
| author_facet | Wei Wang Ying Liu Zi-Hao Zhou Kun Pang Jing-Kai Wang Peng-Fei Huan Jing-Ru Lu Tao Zhu Zuo-Bin Zhu Cong-Hui Han |
| author_sort | Wei Wang |
| collection | DOAJ |
| description | Stem cell treatment may enhance erectile dysfunction (ED) in individuals with cavernous nerve injury (CNI). Nevertheless, no investigations have directly ascertained the implications of varying amounts of human umbilical cord-derived mesenchymal stem cells (HUC-MSCs) on ED. We compare the efficacy of three various doses of HUC-MSCs as a therapeutic strategy for ED. Sprague–Dawley rats (total = 175) were randomly allocated into five groups. A total of 35 rats underwent sham surgery and 140 rats endured bilateral CNI and were treated with vehicles or doses of HUC-MSCs (1 × 106 cells, 5 × 106 cells, and 1 × 10 7 cells in 0.1 ml, respectively). Penile tissues were harvested for histological analysis on 1 day, 3 days, 7 days, 14 days, 28 days, 60 days, and 90 days postsurgery. It was found that varying dosages of HUC-MSCs enhanced the erectile function of rats with bilateral CNI and ED. Moreover, there was no significant disparity in the effectiveness of various dosages of HUC-MSCs. However, the expression of endothelial markers (rat endothelial cell antigen-1 [RECA-1] and endothelial nitric oxide synthase [eNOS]), smooth muscle markers (alpha smooth muscle actin [α-SMA] and desmin), and neural markers (neurofilament [RECA-1] and neurogenic nitric oxide synthase [nNOS]) increased significantly with prolonged treatment time. Masson’s staining demonstrated an increased in the smooth muscle cell (SMC)/collagen ratio. Significant changes were detected in the microstructures of various types of cells. In vivo imaging system (IVIS) analysis showed that at the 1st day, the HUC-MSCs implanted moved to the site of damage. Additionally, the oxidative stress levels were dramatically reduced in the penises of rats administered with HUC-MSCs. |
| format | Article |
| id | doaj-art-3fbee05f9f8146c8ab5b65f90d7ce5d3 |
| institution | Matheson Library |
| issn | 1008-682X 1745-7262 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Journal of Andrology |
| spelling | doaj-art-3fbee05f9f8146c8ab5b65f90d7ce5d32025-07-03T14:52:25ZengWolters Kluwer Medknow PublicationsAsian Journal of Andrology1008-682X1745-72622025-07-0127450851510.4103/aja2024115Effects of human umbilical cord-derived mesenchymal stem cell therapy for cavernous nerve injury-induced erectile dysfunction in the rat modelWei WangYing LiuZi-Hao ZhouKun PangJing-Kai WangPeng-Fei HuanJing-Ru LuTao ZhuZuo-Bin ZhuCong-Hui HanStem cell treatment may enhance erectile dysfunction (ED) in individuals with cavernous nerve injury (CNI). Nevertheless, no investigations have directly ascertained the implications of varying amounts of human umbilical cord-derived mesenchymal stem cells (HUC-MSCs) on ED. We compare the efficacy of three various doses of HUC-MSCs as a therapeutic strategy for ED. Sprague–Dawley rats (total = 175) were randomly allocated into five groups. A total of 35 rats underwent sham surgery and 140 rats endured bilateral CNI and were treated with vehicles or doses of HUC-MSCs (1 × 106 cells, 5 × 106 cells, and 1 × 10 7 cells in 0.1 ml, respectively). Penile tissues were harvested for histological analysis on 1 day, 3 days, 7 days, 14 days, 28 days, 60 days, and 90 days postsurgery. It was found that varying dosages of HUC-MSCs enhanced the erectile function of rats with bilateral CNI and ED. Moreover, there was no significant disparity in the effectiveness of various dosages of HUC-MSCs. However, the expression of endothelial markers (rat endothelial cell antigen-1 [RECA-1] and endothelial nitric oxide synthase [eNOS]), smooth muscle markers (alpha smooth muscle actin [α-SMA] and desmin), and neural markers (neurofilament [RECA-1] and neurogenic nitric oxide synthase [nNOS]) increased significantly with prolonged treatment time. Masson’s staining demonstrated an increased in the smooth muscle cell (SMC)/collagen ratio. Significant changes were detected in the microstructures of various types of cells. In vivo imaging system (IVIS) analysis showed that at the 1st day, the HUC-MSCs implanted moved to the site of damage. Additionally, the oxidative stress levels were dramatically reduced in the penises of rats administered with HUC-MSCs.https://journals.lww.com/10.4103/aja2024115cavernous nerve injurydoseserectile dysfunctionhuman umbilical cord-derived mesenchymal stem cells |
| spellingShingle | Wei Wang Ying Liu Zi-Hao Zhou Kun Pang Jing-Kai Wang Peng-Fei Huan Jing-Ru Lu Tao Zhu Zuo-Bin Zhu Cong-Hui Han Effects of human umbilical cord-derived mesenchymal stem cell therapy for cavernous nerve injury-induced erectile dysfunction in the rat model Asian Journal of Andrology cavernous nerve injury doses erectile dysfunction human umbilical cord-derived mesenchymal stem cells |
| title | Effects of human umbilical cord-derived mesenchymal stem cell therapy for cavernous nerve injury-induced erectile dysfunction in the rat model |
| title_full | Effects of human umbilical cord-derived mesenchymal stem cell therapy for cavernous nerve injury-induced erectile dysfunction in the rat model |
| title_fullStr | Effects of human umbilical cord-derived mesenchymal stem cell therapy for cavernous nerve injury-induced erectile dysfunction in the rat model |
| title_full_unstemmed | Effects of human umbilical cord-derived mesenchymal stem cell therapy for cavernous nerve injury-induced erectile dysfunction in the rat model |
| title_short | Effects of human umbilical cord-derived mesenchymal stem cell therapy for cavernous nerve injury-induced erectile dysfunction in the rat model |
| title_sort | effects of human umbilical cord derived mesenchymal stem cell therapy for cavernous nerve injury induced erectile dysfunction in the rat model |
| topic | cavernous nerve injury doses erectile dysfunction human umbilical cord-derived mesenchymal stem cells |
| url | https://journals.lww.com/10.4103/aja2024115 |
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