SCAR marker linked to resistance gene of powdery mildew in bottle gourd [Lagenaria siceraria (Molina) Standl.] breeding line J083
The genetic analysis of bottle gourd [Lagenaria siceraria (Molina) Standl.] resistance to powdery mildew was evaluated with a highly resistant breeding line J083 and a highly susceptible breeding line J73 and their F<sub>1</sub>, F<sub>2</sub> populations. The results showed...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2011-03-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2011.02.001 |
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| Summary: | The genetic analysis of bottle gourd [Lagenaria siceraria (Molina) Standl.] resistance to powdery mildew was evaluated with a highly resistant breeding line J083 and a highly susceptible breeding line J73 and their F<sub>1</sub>, F<sub>2</sub> populations. The results showed that the resistance to powdery mildew in J083 was controlled by a single recessive gene. From 100 amplified fragment length polymorphism (AFLP) primer pair combinations, a pair of stable polymorphic AFLP markers (E-ATG /M-CTC), 105 bp in size was obtained. The AFLP fragment was then converted to a sequence-characterized amplified region (SCAR) marker, named as GPDS<sub>ATG/CTC75</sub>. This SCAR marker could be used effectively for molecular marker-assisted selection (MAS) in breeding programs to develop bottle gourd cultivars resistance to powdery mildew. |
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| ISSN: | 1008-9209 2097-5155 |