Protective effect of WON-21 on ethanol-induced gastritis in rats
WON-21, an herbal formulation used to alleviate hangover symptoms, has beneficial effects on oxidative stress, inflammation, and alcohol-metabolizing enzymes. This study evaluated the protective effects of WON-21 against ethanol-induced gastritis. In vitro, WON-21 and its constituents, quercetin and...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-09-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464625003196 |
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| author | Do Hwi Park Youngse Oh Won sang Huh Sung Jin Kim Myoung-Sook Shin Chang-Seob Seo Daesik Jeong Ji Hwan Lee Seung Hyun Kim Ki Sung Kang |
| author_facet | Do Hwi Park Youngse Oh Won sang Huh Sung Jin Kim Myoung-Sook Shin Chang-Seob Seo Daesik Jeong Ji Hwan Lee Seung Hyun Kim Ki Sung Kang |
| author_sort | Do Hwi Park |
| collection | DOAJ |
| description | WON-21, an herbal formulation used to alleviate hangover symptoms, has beneficial effects on oxidative stress, inflammation, and alcohol-metabolizing enzymes. This study evaluated the protective effects of WON-21 against ethanol-induced gastritis. In vitro, WON-21 and its constituents, quercetin and apigenin, inhibited TNF-α-induced IL-8 production in human gastric adenocarcinoma cells. In vivo, ethanol-induced gastritis was modeled in rats. Oral pretreatment with 200 and 500 mg/kg WON-21 reduced gastric mucosal lesions by 61.8 % and 79.3 %, respectively. Histopathological analysis revealed a dose-dependent protective effect of WON-21 against inflammatory cell infiltration and epithelial damage. WON-21 also significantly decreased IL-6 levels and suppressed the mRNA expression of IL-8 and IL-18 in gastric tissues. Network pharmacology analysis was performed to elucidate key targets and pathways, supporting these findings. The gastroprotective efficacy of the high dose (500 mg/kg) was comparable to that of omeprazole. These results demonstrate that WON-21 exerts anti-inflammatory and mucosa-preserving effects against ethanol-induced gastritis. |
| format | Article |
| id | doaj-art-3b08189e9c69481f8d33c07af2046b6c |
| institution | Matheson Library |
| issn | 1756-4646 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj-art-3b08189e9c69481f8d33c07af2046b6c2025-08-02T04:46:45ZengElsevierJournal of Functional Foods1756-46462025-09-01132106977Protective effect of WON-21 on ethanol-induced gastritis in ratsDo Hwi Park0Youngse Oh1Won sang Huh2Sung Jin Kim3Myoung-Sook Shin4Chang-Seob Seo5Daesik Jeong6Ji Hwan Lee7Seung Hyun Kim8Ki Sung Kang9College of Korean Medicine, Gachon University, Seongnam 13120, Republic of KoreaCollege of Pharmacy, Yonsei Institute of Pharmaceutical Science, Yonsei University, Incheon, 21983, Republic of KoreaBacchuslab Co. Ltd., 44, Achasan-ro 78-gil, Gwangjin-gu, Seoul 04969, Republic of KoreaCollege of Korean Medicine, Gachon University, Seongnam 13120, Republic of KoreaCollege of Korean Medicine, Gachon University, Seongnam 13120, Republic of KoreaHerbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, South KoreaNational Center of Excellence in Software, Faculty of SW Convergence, College of Convergence Engineering, Sangmyung University, Seoul, Republic of KoreaCollege of Korean Medicine, Gachon University, Seongnam 13120, Republic of KoreaCollege of Pharmacy, Yonsei Institute of Pharmaceutical Science, Yonsei University, Incheon, 21983, Republic of Korea; Corresponding authors.College of Korean Medicine, Gachon University, Seongnam 13120, Republic of Korea; Corresponding authors.WON-21, an herbal formulation used to alleviate hangover symptoms, has beneficial effects on oxidative stress, inflammation, and alcohol-metabolizing enzymes. This study evaluated the protective effects of WON-21 against ethanol-induced gastritis. In vitro, WON-21 and its constituents, quercetin and apigenin, inhibited TNF-α-induced IL-8 production in human gastric adenocarcinoma cells. In vivo, ethanol-induced gastritis was modeled in rats. Oral pretreatment with 200 and 500 mg/kg WON-21 reduced gastric mucosal lesions by 61.8 % and 79.3 %, respectively. Histopathological analysis revealed a dose-dependent protective effect of WON-21 against inflammatory cell infiltration and epithelial damage. WON-21 also significantly decreased IL-6 levels and suppressed the mRNA expression of IL-8 and IL-18 in gastric tissues. Network pharmacology analysis was performed to elucidate key targets and pathways, supporting these findings. The gastroprotective efficacy of the high dose (500 mg/kg) was comparable to that of omeprazole. These results demonstrate that WON-21 exerts anti-inflammatory and mucosa-preserving effects against ethanol-induced gastritis.http://www.sciencedirect.com/science/article/pii/S1756464625003196Herbal medicineAcute gastric injuryNetwork pharmacological analysisIL-8IL-18 |
| spellingShingle | Do Hwi Park Youngse Oh Won sang Huh Sung Jin Kim Myoung-Sook Shin Chang-Seob Seo Daesik Jeong Ji Hwan Lee Seung Hyun Kim Ki Sung Kang Protective effect of WON-21 on ethanol-induced gastritis in rats Journal of Functional Foods Herbal medicine Acute gastric injury Network pharmacological analysis IL-8 IL-18 |
| title | Protective effect of WON-21 on ethanol-induced gastritis in rats |
| title_full | Protective effect of WON-21 on ethanol-induced gastritis in rats |
| title_fullStr | Protective effect of WON-21 on ethanol-induced gastritis in rats |
| title_full_unstemmed | Protective effect of WON-21 on ethanol-induced gastritis in rats |
| title_short | Protective effect of WON-21 on ethanol-induced gastritis in rats |
| title_sort | protective effect of won 21 on ethanol induced gastritis in rats |
| topic | Herbal medicine Acute gastric injury Network pharmacological analysis IL-8 IL-18 |
| url | http://www.sciencedirect.com/science/article/pii/S1756464625003196 |
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