Resveratrol alleviates oxidative stress, inflammatory cytokine expression and apoptosis induced by nanoplastics in the hemocytes of abalone (Haliotis discus hannai)
The impact of nanoplastics (NPs) on molluskan hemocytes and the potential of resveratrol (RES) to counteract such effects require further investigation. The aim of the present study was to evaluate the effects of RES on oxidative stress, inflammatory cytokine expression and apoptosis induced by NPs...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-09-01
|
| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513425003692 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1839629555925516288 |
|---|---|
| author | Jiping Zhang Qiyuan Fu Yingjie Wang Ye Mu Hai Ren Liyuan Xue Jiahuan Liu |
| author_facet | Jiping Zhang Qiyuan Fu Yingjie Wang Ye Mu Hai Ren Liyuan Xue Jiahuan Liu |
| author_sort | Jiping Zhang |
| collection | DOAJ |
| description | The impact of nanoplastics (NPs) on molluskan hemocytes and the potential of resveratrol (RES) to counteract such effects require further investigation. The aim of the present study was to evaluate the effects of RES on oxidative stress, inflammatory cytokine expression and apoptosis induced by NPs in primary cultured hemocytes of abalone (Haliotis discus hannai). In this study, hemocytes of abalone were treated with control medium (control group), medium containing 100 μg/mL NPs (NPs group) or 100 μg/mL NPs + 1.14 μg/mL RES (NPs + RES group) in vitro for 24 h, respectively. Results showed that RES significantly restored the NPs-induced reduction in catalase and superoxide dismutase activity. Additionally, RES mitigated the increase of intracellular reactive oxygen species and malondialdehyde content induced by NPs. Relative mRNA expression of inflammatory genes (nf-κb, tnf-α, il-16 and il-17) and pro-apoptotic factor (caspase-3) were significantly up-regulated in the NPs group, while RES addition decreased the expression of these genes. RES also restored the NPs-induced reduction of antioxidant (nrf2, cat and cuznsod), anti-apoptotic (bcl-2) genes and SIRT1 protein expression. Meanwhile, the protein levels of ASC, cleaved caspase-3, IL-1β and nuclear translocation of NF-κB p65 induced by NPs was suppressed by RES addition. These results demonstrated that RES alleviated oxidative stress, inflammatory cytokine expression and apoptosis induced by NPs in abalone hemocytes. The Keap1/Nrf2, NF-κB and Bax/Bcl-2/caspase-3 signaling pathways were the main targets of the biological effects of RES in the presence of NPs. |
| format | Article |
| id | doaj-art-35ad4b532c064c72b6e63bddcd586f8c |
| institution | Matheson Library |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-35ad4b532c064c72b6e63bddcd586f8c2025-07-15T04:16:15ZengElsevierAquaculture Reports2352-51342025-09-0143102983Resveratrol alleviates oxidative stress, inflammatory cytokine expression and apoptosis induced by nanoplastics in the hemocytes of abalone (Haliotis discus hannai)Jiping Zhang0Qiyuan Fu1Yingjie Wang2Ye Mu3Hai Ren4Liyuan Xue5Jiahuan Liu6College of Marine Resources and Environment, Hebei Normal University of Science and Technology, Qinhuangdao 066000, ChinaDepartment of Microbiological Detection, Qinhuangdao Center for Disease Control and Prevention, Qinhuangdao 066000, ChinaCollege of Marine Resources and Environment, Hebei Normal University of Science and Technology, Qinhuangdao 066000, ChinaCollege of Marine Resources and Environment, Hebei Normal University of Science and Technology, Qinhuangdao 066000, ChinaCollege of Marine Resources and Environment, Hebei Normal University of Science and Technology, Qinhuangdao 066000, China; Hebei Key Laboratory of Ocean Dynamics, Resources and Environments, Hebei Normal University of Science and Technology, Qinhuangdao 066000, ChinaCollege of Marine Resources and Environment, Hebei Normal University of Science and Technology, Qinhuangdao 066000, China; Hebei Key Laboratory of Ocean Dynamics, Resources and Environments, Hebei Normal University of Science and Technology, Qinhuangdao 066000, ChinaCollege of Marine Resources and Environment, Hebei Normal University of Science and Technology, Qinhuangdao 066000, China; Hebei Key Laboratory of Ocean Dynamics, Resources and Environments, Hebei Normal University of Science and Technology, Qinhuangdao 066000, China; Corresponding author at: College of Marine Resources and Environment, Hebei Normal University of Science and Technology, Qinhuangdao 066000, China.The impact of nanoplastics (NPs) on molluskan hemocytes and the potential of resveratrol (RES) to counteract such effects require further investigation. The aim of the present study was to evaluate the effects of RES on oxidative stress, inflammatory cytokine expression and apoptosis induced by NPs in primary cultured hemocytes of abalone (Haliotis discus hannai). In this study, hemocytes of abalone were treated with control medium (control group), medium containing 100 μg/mL NPs (NPs group) or 100 μg/mL NPs + 1.14 μg/mL RES (NPs + RES group) in vitro for 24 h, respectively. Results showed that RES significantly restored the NPs-induced reduction in catalase and superoxide dismutase activity. Additionally, RES mitigated the increase of intracellular reactive oxygen species and malondialdehyde content induced by NPs. Relative mRNA expression of inflammatory genes (nf-κb, tnf-α, il-16 and il-17) and pro-apoptotic factor (caspase-3) were significantly up-regulated in the NPs group, while RES addition decreased the expression of these genes. RES also restored the NPs-induced reduction of antioxidant (nrf2, cat and cuznsod), anti-apoptotic (bcl-2) genes and SIRT1 protein expression. Meanwhile, the protein levels of ASC, cleaved caspase-3, IL-1β and nuclear translocation of NF-κB p65 induced by NPs was suppressed by RES addition. These results demonstrated that RES alleviated oxidative stress, inflammatory cytokine expression and apoptosis induced by NPs in abalone hemocytes. The Keap1/Nrf2, NF-κB and Bax/Bcl-2/caspase-3 signaling pathways were the main targets of the biological effects of RES in the presence of NPs.http://www.sciencedirect.com/science/article/pii/S2352513425003692Molluskan hemocytesNanoplasticsResveratrolAnti-oxidationApoptosis |
| spellingShingle | Jiping Zhang Qiyuan Fu Yingjie Wang Ye Mu Hai Ren Liyuan Xue Jiahuan Liu Resveratrol alleviates oxidative stress, inflammatory cytokine expression and apoptosis induced by nanoplastics in the hemocytes of abalone (Haliotis discus hannai) Aquaculture Reports Molluskan hemocytes Nanoplastics Resveratrol Anti-oxidation Apoptosis |
| title | Resveratrol alleviates oxidative stress, inflammatory cytokine expression and apoptosis induced by nanoplastics in the hemocytes of abalone (Haliotis discus hannai) |
| title_full | Resveratrol alleviates oxidative stress, inflammatory cytokine expression and apoptosis induced by nanoplastics in the hemocytes of abalone (Haliotis discus hannai) |
| title_fullStr | Resveratrol alleviates oxidative stress, inflammatory cytokine expression and apoptosis induced by nanoplastics in the hemocytes of abalone (Haliotis discus hannai) |
| title_full_unstemmed | Resveratrol alleviates oxidative stress, inflammatory cytokine expression and apoptosis induced by nanoplastics in the hemocytes of abalone (Haliotis discus hannai) |
| title_short | Resveratrol alleviates oxidative stress, inflammatory cytokine expression and apoptosis induced by nanoplastics in the hemocytes of abalone (Haliotis discus hannai) |
| title_sort | resveratrol alleviates oxidative stress inflammatory cytokine expression and apoptosis induced by nanoplastics in the hemocytes of abalone haliotis discus hannai |
| topic | Molluskan hemocytes Nanoplastics Resveratrol Anti-oxidation Apoptosis |
| url | http://www.sciencedirect.com/science/article/pii/S2352513425003692 |
| work_keys_str_mv | AT jipingzhang resveratrolalleviatesoxidativestressinflammatorycytokineexpressionandapoptosisinducedbynanoplasticsinthehemocytesofabalonehaliotisdiscushannai AT qiyuanfu resveratrolalleviatesoxidativestressinflammatorycytokineexpressionandapoptosisinducedbynanoplasticsinthehemocytesofabalonehaliotisdiscushannai AT yingjiewang resveratrolalleviatesoxidativestressinflammatorycytokineexpressionandapoptosisinducedbynanoplasticsinthehemocytesofabalonehaliotisdiscushannai AT yemu resveratrolalleviatesoxidativestressinflammatorycytokineexpressionandapoptosisinducedbynanoplasticsinthehemocytesofabalonehaliotisdiscushannai AT hairen resveratrolalleviatesoxidativestressinflammatorycytokineexpressionandapoptosisinducedbynanoplasticsinthehemocytesofabalonehaliotisdiscushannai AT liyuanxue resveratrolalleviatesoxidativestressinflammatorycytokineexpressionandapoptosisinducedbynanoplasticsinthehemocytesofabalonehaliotisdiscushannai AT jiahuanliu resveratrolalleviatesoxidativestressinflammatorycytokineexpressionandapoptosisinducedbynanoplasticsinthehemocytesofabalonehaliotisdiscushannai |